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DOI :
10.3791/57059-v
•
11:19 min
March 20th, 2018
Chapters
0:04
Title
1:16
Ultramicrotome and Sample Setup
3:04
Ultramicrotome Sectioning
4:21
Hierarchical Imaging in the SEM
7:33
Registration of the SEM Image Stack
8:39
Results: Multimodal Hierarchical Imaging of Serial Sections of Plant Roots
10:06
Conclusion
本协议以扫描电镜 (SEM) 为目标, 以纳米尺度分辨率成像组织中的特定细胞为靶点。从树脂嵌入的生物材料中, 大量的系列部分首先在光学显微镜中被成像, 以识别目标, 然后以分层的方式在 SEM 中。
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