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DOI :
10.3791/57069-v
April 9th, 2018
Chapters
0:04
Title
1:11
Fluorescence-based Screening of Incorporation Efficiencies
4:22
Genetic Incorporation of ncAAs into GPCRs
8:21
Ultrafast Bioorthogonal Labeling of GPCRs on Live Mammalian Cells
11:11
Results: Incorporation of Chemical Probes into GPCRs in Mammalian Cells for Cross-linking and Imaging Studies
12:52
Conclusion
本文提出了一种简便的荧光分析方法, 用于评价将非规范氨基酸 (ncAAs) 纳入哺乳动物细胞表达的蛋白质中的氨基酰 tRNA 合成酶/tRNA 对的效率。介绍了 ncAAs 在 G 蛋白耦合受体 (受体) 研究中的应用, 包括结合位点的光交联映射和活细胞的 bioorthogonal GPCR 标记。
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