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DOI :
10.3791/57077-v
May 24th, 2018
Chapters
0:05
Title
0:45
Ex Vivo Infection
1:48
Hemolymph Extraction
3:11
In Vivo Infection
4:08
Hemolymph Extraction and Hemocyte Plating
4:35
Confocal Imaging
5:44
3D Model Reconstruction
6:58
Results: Dynamic Visualization of Drosophila Hemocytes
8:09
Conclusion
该方法展示了如何用三维 (3D) 模型来可视化病原体侵入昆虫细胞。包囊从果蝇幼虫感染病毒或细菌病原体, 无论是体内或体内。感染的包囊然后固定和染色的成像与共聚焦显微镜和随后的3D 细胞重建。
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