Name: in vivo morphometric analysis of human cranial nerves using magnetic resonance imaging in meni&#232;re's disease ears and normal hearing ears
Uploaded: 2018-02-21T15:00:00
Description: Watch this Scientific Journal Video about In Vivo Morphometric Analysis of Human Cranial Nerves Using Magnetic Resonance Imaging in MeniÃƒÂ¨re's Disease Ears and Normal Hearing Ears at JoVE.com

罗伯特 Gürkov 接受了拨款从德国研究和教育部 BMBF, 格兰特01号0901。

所有程序均经当地道德委员会批准 (慕尼黑大学/LMU 慕尼黑093-09 号议定书机构审查委员会)。所有患者都对所执行的程序给予知情同意。
1. 临床检查
<ol><li>识别患有疑似 MD 的患者, 与该部门合作进行耳鼻喉部的治疗。<ol>
<li>进行临床评估;眩晕, 耳鸣/耳铃, 听力损失 (可能波动) 需要评估。检查相关的恶心和呕吐。检查症状的持续时间。</li>
		<li>将临床和功能测试结果考虑到对 M...

<ol>
	<li>Gurkov, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vivo+visualized+endolymphatic+hydrops+and+inner+ear+functions+in+patients+with+electrocochleographically+confirmed+Meniere's+disease.">In vivo visualized endolymphatic hydrops and inner ear functions in patients with electrocochleographically confirmed Meniere's disease.</a> Otol Neurotol. 33 (6), 1040-1045 (2012).</li><li>Gurkov, R., Flatz, W., Louza, J., Strupp, M., Krause, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vivo+visualization+of+endolyphatic+hydrops+in+patients+with+Meniere's+disease:+correlation+with+audiovestibular+function.">In vivo visualization of endolyphatic hydrops in patients with Meniere's disease: correlation with audiovestibular function.</a> Eur Arch Otorhinolaryngol. 268 (12), 1743-1748 (2011).</li><li>Gurkov, R., Pyyko, I., Zou, J., Kentala, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=What+is+Meniere's+disease?+A+contemporary+re-evaluation+of+endolymphatic+hydrops.">What is Meniere's disease? A contemporary re-evaluation of endolymphatic hydrops.</a> J Neurol. 263, 71-81 (2016).</li><li>Plontke, S. K., Gurkov, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Meniere's+Disease.">Meniere's Disease.</a> Laryngorhinootologie. 94 (8), 530-554 (2015).</li><li>Klockars, T., Kentala, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Inheritance+of+Meniere's+disease+in+the+Finnish+population.">Inheritance of Meniere's disease in the Finnish population.</a> Arch Otolaryngol Head Neck Surg. 133 (1), 73-77 (2007).</li><li>Greco, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Meniere's+disease+might+be+an+autoimmune+condition.">Meniere's disease might be an autoimmune condition.</a> Autoimmun Rev. 11 (10), 731-738 (2012).</li><li>Ozdogmus, O., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Connections+between+the+facial,+vestibular+and+cochlear+nerve+bundles+within+the+internal+auditory+canal.">Connections between the facial, vestibular and cochlear nerve bundles within the internal auditory canal.</a> J Anat. 205 (1), 65-75 (2004).</li><li>Nakamichi, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Establishing+normal+diameter+range+of+the+cochlear+and+facial+nerves+with+3D-CISS+at+3T.">Establishing normal diameter range of the cochlear and facial nerves with 3D-CISS at 3T.</a> Magn Reson Med Sci. 12 (4), 241-247 (2013).</li><li>Kang, W. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Normative+diameters+and+effects+of+aging+on+the+cochlear+and+facial+nerves+in+normal-hearing+Korean+ears+using+3.0-tesla+magnetic+resonance+imaging.">Normative diameters and effects of aging on the cochlear and facial nerves in normal-hearing Korean ears using 3.0-tesla magnetic resonance imaging.</a> Laryngoscope. 122 (5), 1109-1114 (2012).</li><li>Jaryszak, E. M., Patel, N. A., Camp, M., Mancuso, A. A., Antonelli, P. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cochlear+nerve+diameter+in+normal+hearing+ears+using+high-resolution+magnetic+resonance+imaging.">Cochlear nerve diameter in normal hearing ears using high-resolution magnetic resonance imaging.</a> Laryngoscope. 119 (10), 2042-2045 (2009).</li><li>Henneberger, A., Ertl-Wagner, B., Reiser, M., Gurkov, R., Flatz, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Morphometric+evaluation+of+facial+and+vestibulocochlear+nerves+using+magnetic+resonance+imaging:+comparison+of+Meniere's+disease+ears+with+normal+hearing+ears.">Morphometric evaluation of facial and vestibulocochlear nerves using magnetic resonance imaging: comparison of Meniere's disease ears with normal hearing ears.</a> Eur Arch Otorhinolaryngol. 274 (8), 3029-3039 (2017).</li><li>Zwergal, A., Brandt, T., Magnusson, M., Kennard, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=DIZZYNET:+the+European+network+for+vertigo+and+balance+research.">DIZZYNET: the European network for vertigo and balance research.</a> J Neurol. 263, 1 (2016).</li><li>Zwergal, A., Brandt, T., Magnusson, M., Kennard, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=DIZZYNET--a+European+network+initiative+for+vertigo+and+balance+research:+visions+and+aims.">DIZZYNET--a European network initiative for vertigo and balance research: visions and aims.</a> J Neurol. 263, 2-9 (2016).</li><li>Grill, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=DizzyReg:+the+prospective+patient+registry+of+the+German+Center+for+Vertigo+and+Balance+Disorders.">DizzyReg: the prospective patient registry of the German Center for Vertigo and Balance Disorders.</a> J Neurol. , (2017).</li><li>Semaan, M. T., Alagramam, K. N., Megerian, C. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+basic+science+of+Meniere's+disease+and+endolymphatic+hydrops.">The basic science of Meniere's disease and endolymphatic hydrops.</a> Curr Opin Otolaryngol Head Neck Surg. 13 (5), 301-307 (2005).</li><li>Hayashi, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Usefulness+of+the+advanced+neuroimaging+protocol+based+on+plain+and+gadolinium-enhanced+constructive+interference+in+steady+state+images+for+gamma+knife+radiosurgery+and+planning+microsurgical+procedures+for+skull+base+tumors.">Usefulness of the advanced neuroimaging protocol based on plain and gadolinium-enhanced constructive interference in steady state images for gamma knife radiosurgery and planning microsurgical procedures for skull base tumors.</a> Acta Neurochir Suppl. 116, 167-178 (2013).</li></ol>

我们已经展示了一种可行的和可接近的方法评估颅神经的形态学变化, 因为它们可能发生在几个病理生理学的情况下, 这里在 MD 与正常听力控制。
修改和疑难解答:
对在这里报告的 7th和 8th颅神经的类似测量可以使用对不同级别的所有其他颅神经进行的3维连序列扫描, 只要它们仍然被脑液包围,否则, 与上述 MR 扫描序列技术可能...

磁共振成像 (MRI) 在人体解剖学和生理病理过程的可视化和分析中起着重要的作用。由于 Menière 疾病 (MD) 的临床和电生理诊断可能具有挑战性, 因此, 使用来自 MRI 的附加信息比帮助1、2、3、4更有用。采用体内方法, 通过 MRI 对 MD 淋巴积液和颅神经形态变化进行分析。采用这种联合方法, 对确定 MD 的诊断进行了验证, 并在整个神经过程中对颅神经的形态学变化进行了不同程度的研究。MD 的病因仍然不清楚5,6,7。有人建议, 神经细胞丧失可能涉及 MD, 但这还有待证实。
本研究分析了在 MD 中, 适合的颅神经用于形态学分析的7个th和8个th神经及其分支。只有少数研究可以被发现分析这些神经的形态学方面使用 MRI8,9,10。Henneberger et . 的研究分析了 7th和 8th脑神经在 MD 耳中的形态学变化, 与正常的听觉耳朵11相比。
这里提出的方法使在体内可视化和形态学分析的 7th和 8th脑神经在整个过程中从大脑到颞骨。使用这种方法, 我们发现 MD 患者组与健康耳有显著差异。我们建议在几种情况下使用的描述方法, 只要潜在的形态学变化的颅神经感兴趣。这种方法是否将建立在临床诊断资料仍有待评估的未来研究。实际的替代方法为在体内评估颅骨神经形态学变化, 而计算机断层扫描 (CT) 有它的优势, 如广泛的可用性, 速度和骨骼变化的描述, 它也表现出太低的组织对比, 以想象在脑颅和颞骨的颅神经微妙的变化。尸检后对 MD 患者颅神经变化的分析有待研究。以特殊的影像和评价技术为例, 可以分析 MD 患者颅神经的形态学变化和健康控制的 MRI。常规 MRI 检查的大脑往往不包括高分辨率, 强烈 T2-weighted 成像技术, 这是强制性的评估形态学变化的颅神经7和8。
所开发的方法可能进一步诊断的影响, 以评估不同程度的严重性在 MD, 以及发挥作用, 评估眩晕, 听力缺损, 和耳鸣。眩晕诊断和治疗检查专门中心在今天的医疗保健系统中起着重要作用, 我们的方法可以为专家提供诊断检查的可能工具12,13,14.眩晕是一种复杂的症状发生在一些疾病, 需要一个彻底的跨学科合作, 不同的专业, 如在诊断和治疗眩晕的专门中心显示12,13,14。
根据我们的知识, 在文献中没有任何方法可用于在医学和健康控制中对颅神经进行体内形态学分析。

<table border="0" cellpadding="0" cellspacing="0" style="width:572px;" width="573">
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		<col />
		<col />
		<col />
		<col />
	</colgroup>
	<tbody>
		<tr height="189">
			<td height="189" style="height:189px;width:216px;">MR-scanner, e.g. Siemens Magnetom Verio, or appropriate MR-scans in DICOM format, e.g. 3D-CISS</td>
			<td style="width:71px;">Siemens Healthcare GmbH, Erlangen, Germany, or MR scans by any other vendor</td>
			<td align="right" style="width:119px;">1</td>
			<td style="width:167px;">Instead of the MR scanner, appropriately acquired MR-scans can be used for morphometric analysis</td>
		</tr>
		<tr height="105">
			<td height="105" style="height:105px;width:216px;">Osirix or any other DICOM-Viewer with appropriate evaluation tools</td>
			<td style="width:71px;">Pixmeo SARL, Geneva, Switzerland</td>
			<td align="right" style="width:119px;">2</td>
			<td>Software for viewing and evaluating DICOM images</td>
		</tr>
		<tr height="105">
			<td height="105" style="height:105px;width:216px;">MedCalc or any other statistical analysis software, e.g. SPSS&#160;</td>
			<td style="width:71px;">MedCalc Software bvba, Ostend, Belgium</td>
			<td align="right" style="width:119px;">3</td>
			<td>Software for statistical analysis</td>
		</tr>
		<tr height="105">
			<td height="105" style="height:105px;width:216px;">Computer running Windows or MacOSX/macOS</td>
			<td style="width:71px;">e.g. Lenovo, Apple or anything selfmade</td>
			<td align="right" style="width:119px;">4</td>
			<td>Hardware on which the above software can be employed</td>
		</tr>
	</tbody>
</table>

in vivo morphometric analysis of human cranial nerves using magnetic resonance imaging in meni&#232;re's disease ears and normal hearing ears

Menière 病 (MD) 的神经结构分析是非常重要的, 因为这种结构的损失以前已经提出给这个病人组, 但尚未证实。本协议描述了一种在体内评估神经变化的方法, 特别适用于磁共振成像 (MRI) 颅神经分析。使用扫描协议 (包括强 T2-weighted 3D 梯度-回声序列 (3 连), 在3吨 MR 扫描仪中检查 MD 患者和正常听力者。在患者组中, 通过 MRI 对淋巴积液的评估, 进一步证实了 MD。形态学分析是使用免费的 DICOM 查看器进行的。颅神经的评估包括测量不同级别神经的横截面积 (csa) 以及正交直径测量。

采用统计分析软件进行统计分析, 应用双侧独立样本t测试。图像评价由两个读者进行。患者组平均值 (n = 21) 和健康对照组 (n = 39) 之间的显著差异可用于测量面神经、CN、SVN 和 IVN (表 2) 的 CSA。csa 在患者组中的测量显示了显著较大的 csa 值 (图 2和图 3)。对 ld 和 sd 的测量结果进行了不同的评估, 根据测量地点?...

Watch this Scientific Journal Video about In Vivo Morphometric Analysis of Human Cranial Nerves Using Magnetic Resonance Imaging in MeniÃƒÂ¨re's Disease Ears and Normal Hearing Ears at JoVE.com

In Vivo Morphometric Analysis of Human Cranial Nerves Using Magnetic Resonance Imaging in MeniÃƒÆ’Ã†'Ãƒâ€šÃ‚Â¨re's Disease Ears and Normal Hearing Ears

体内Menière 病耳和正常听觉的磁共振成像对人颅神经的形态学分析

目的评价颅神经的形态学变化, 如神经结构丧失或颅神经肿胀 (MD) 或健康人在体内, 采用磁共振成像 (MRI) 建立了 Menière 的评价协议。.另外还对 MD 进行了 MRI 诊断。

Analysis of neural structures in Meni&#232;re's Disease (MD) is of importance, since a loss of such structures has previously been proposed for this ...

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In Vivo Morphometric Analysis of Human Cranial Nerves Using Magnetic Resonance Imaging in Meni&#232;re's Disease Ears and Normal Hearing Ears

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