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DOI :
10.3791/57109-v
June 15th, 2018
Chapters
0:04
Title
0:45
Plasmid Cloning
2:54
Cell Culture and Transfection
3:41
Harvesting Lysates
4:31
Affinity Purification and Preparation of BiCAP Eluant for Western Blotting
5:22
Results: BiCAP Method is a Powerful Technique to Specifically Purify and Characterize Interacting Proteins
6:06
Conclusion
本稿では、二分子の相補性の親和性の浄化 (BiCAP) のプロトコルについて説明します。この手法は競合結合パートナーで形成された複合体と同様、国連複合体の個々 のタンパク質を除く、特定の分離と任意の 2 つの相互作用の蛋白質の下流のプロテオーム解析を促進します。
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