Name: an experimental model of diet-induced metabolic syndrome in rabbit: methodological considerations, development, and assessment
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Dette arbejde blev støttet af Generalitat Valenciana (GV2015-062), Universitat de València (UV-INV-PRECOMP14-206372) til MZ, Generalitat Valenciana (PROMETEOII/2014/037) og Instituto de Salud Carlos III-FEDER midler (CIBERCV CB16/11/0486) til FJC.

Pasning af dyr og de eksperimentelle protokoller, der bruges i denne undersøgelse overholdt EU direktiv 2010/63 om beskyttelse af dyr, der anvendes til videnskabelige formål, og blev godkendt af det institutionelle dyrs pleje og brug udvalg (2015/VSC/ærter/00049).
Bemærk: Protokollen består af kronisk indgift af et højt fedtindhold, høj-saccharose diet til 28 uger, og vurderingen af de vigtigste komponenter i MetS. Vi brugte 11 voksne mandlige New Zealand White (NZW) kaniner vejer 4,39 ...

<ol>
	<li>Cornier, M. A., Dabelea, D., Hernandez, T. L., Lindstrom, R. C., Steig, A. J., Stob, N. R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+metabolic+syndrome.">The metabolic syndrome.</a> Endocr rev. 29 (7), 777-822 (2008).</li><li>. IDF Consensus Worldwide Definition of the Metabolic Syndrome Available from: <a target="_blank" href="https://www.idf.org/e-library/consensus-statements.html">https://www.idf.org/e-library/consensus-statements.html</a> (2006)</li><li>Alberti, K. G., Eckel, R. H., Grundy, S. M., Zimmet, P. Z., Cleeman, J. I., Donato, K. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Harmonizing+the+metabolic+syndrome:+a+joint+interim+statement+of+the+International+Diabetes+Federation+Task+Force+on+Epidemiology+and+Prevention;+National+Heart,+Lung,+and+Blood+Institute;+American+Heart+Association;+World+Heart+Federation;+International+Atherosclerosis+Society;+and+International+Association+for+the+Study+of+Obesity.">Harmonizing the metabolic syndrome: a joint interim statement of the International Diabetes Federation Task Force on Epidemiology and Prevention; National Heart, Lung, and Blood Institute; American Heart Association; World Heart Federation; International Atherosclerosis Society; and International Association for the Study of Obesity.</a> Circulation. 120 (16), 1640-1645 (2009).</li><li>Grundy, S. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pre-diabetes,+metabolic+syndrome,+and+cardiovascular+risk.">Pre-diabetes, metabolic syndrome, and cardiovascular risk.</a> JACC. 59 (7), 635-643 (2012).</li><li>Verkest, K. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Is+the+metabolic+syndrome+a+useful+clinical+concept+in+dogs?+A+review+of+the+evidence.">Is the metabolic syndrome a useful clinical concept in dogs? A review of the evidence.</a> Vet J. 199 (1), 24-30 (2014).</li><li>Zhang, X., Lerman, L. O. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Investigating+the+Metabolic+Syndrome:+Contributions+of+Swine+Models.">Investigating the Metabolic Syndrome: Contributions of Swine Models.</a> Toxicol Pathol. 44 (3), 358-366 (2016).</li><li>Wong, S. K., Chin, K. Y., Suhaimi, F. H., Fairus, A., Ima-Nirwana, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Animal+models+of+metabolic+syndrome:+a+review.">Animal models of metabolic syndrome: a review.</a> Nutr Metab (Lond). 13, 65 (2016).</li><li>Carroll, J. F., Dwyer, T. M., Grady, A. W., Reinhart, G. A., Montani, J. P., Cockrell, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hypertension,+cardiac+hypertrophy,+and+neurohumoral+activity+in+a+new+animal+model+of+obesity.">Hypertension, cardiac hypertrophy, and neurohumoral activity in a new animal model of obesity.</a> Am J Physiol. 271 (1 Pt 2), H373-H378 (1996).</li><li>Grooth, G. J., Klerkx, A. H., Stroes, E. S., Stalenhoef, A. F., Kastelein, J. J., Kuivenhoven, J. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+review+of+CETP+and+its+relation+to+atherosclerosis.">A review of CETP and its relation to atherosclerosis.</a> J Lipid Res. 45 (11), 1967-1974 (2004).</li><li>Zarzoso, M., Mironov, S., Guerrero-Serna, G., Willis, B. C., Pandit, S. V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ventricular+remodelling+in+rabbits+with+sustained+high-fat+diet.">Ventricular remodelling in rabbits with sustained high-fat diet.</a> Acta Physiol (Oxf). 211 (1), 36-47 (2014).</li><li>Filippi, S., Vignozzi, L., Morelli, A., Chavalmane, A. K., Sarchielli, E., Fibbi, B., Saad, F., Sandner, P., Ruggiano, P., Vannelli, G. B., Mannucci, E., Maggi, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Testosterone+partially+ameliorates+metabolic+profile+and+erectile+responsiveness+to+PDE5+inhibitors+in+an+animal+model+of+male+metabolic+syndrome.">Testosterone partially ameliorates metabolic profile and erectile responsiveness to PDE5 inhibitors in an animal model of male metabolic syndrome.</a> J Sex Med. 6 (12), 3274-3288 (2009).</li><li>Waqar, A. B., Koike, T., Yu, Y., Inoue, T., Aoki, T., Liu, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=High-fat+diet+without+excess+calories+induces+metabolic+disorders+and+enhances+atherosclerosis+in+rabbits.">High-fat diet without excess calories induces metabolic disorders and enhances atherosclerosis in rabbits.</a> Atherosclerosis. 213 (1), 148-155 (2010).</li><li>Fan, J., Watanabe, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cholesterol-fed+and+transgenic+rabbit+models+for+the+study+of+atherosclerosis.">Cholesterol-fed and transgenic rabbit models for the study of atherosclerosis.</a> J Atheroscler Thromb. 7 (1), 26-32 (2000).</li><li>Yin, W., Yuan, Z., Wang, Z., Yang, B., Yang, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+diet+high+in+saturated+fat+and+sucrose+alters+glucoregulation+and+induces+aortic+fatty+streaks+in+New+Zealand+White+rabbits.">A diet high in saturated fat and sucrose alters glucoregulation and induces aortic fatty streaks in New Zealand White rabbits.</a> Int J Exp Diabetes Res. 3 (3), 179-184 (2002).</li><li>Zhao, S., Chu, Y., Zhang, C., Lin, Y., Xu, K., Yang, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Diet-induced+central+obesity+and+insulin+resistance+in+rabbits.">Diet-induced central obesity and insulin resistance in rabbits.</a> J Anim Physiol Anim Nutr (Berl). 92 (1), 105-111 (2008).</li><li>Helfenstein, T., Fonseca, F. A., Ihara, S. S., Bottos, J. M., Moreira, F. T., Pott, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Impaired+glucose+tolerance+plus+hyperlipidaemia+induced+by+diet+promotes+retina+microaneurysms+in+New+Zealand+rabbits.">Impaired glucose tolerance plus hyperlipidaemia induced by diet promotes retina microaneurysms in New Zealand rabbits.</a> Int J Exp Pathol. 92 (1), 40-49 (2011).</li><li>Ning, B., Wang, X., Yu, Y., Waqar, A. B., Yu, Q., Koike, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=High-fructose+and+high-fat+diet-induced+insulin+resistance+enhances+atherosclerosis+in+Watanabe+heritable+hyperlipidemic+rabbits.">High-fructose and high-fat diet-induced insulin resistance enhances atherosclerosis in Watanabe heritable hyperlipidemic rabbits.</a> Nutr Metab (Lond). 12, 30 (2015).</li><li>Liu, Y., Li, B., Li, M., Yu, Y., Wang, Z., Chen, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Improvement+of+cardiac+dysfunction+by+bilateral+surgical+renal+denervation+in+animals+with+diabetes+induced+by+high+fructose+and+high+fat+diet.">Improvement of cardiac dysfunction by bilateral surgical renal denervation in animals with diabetes induced by high fructose and high fat diet.</a> Diabetes Res Clin Pract. 115, 140-149 (2016).</li><li>Arias-Mutis, O. J., Marrachelli, V. G., Ruiz-Saur&#237;, A., Alberola, A., Morales, J. M., Such-Miquel, L., Monleon, D., Chorro, F. J., Such, L., Zarzoso, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Development+and+characterization+of+an+experimental+model+of+diet-induced+metabolic+syndrome+in+rabbit.">Development and characterization of an experimental model of diet-induced metabolic syndrome in rabbit.</a> PLoS One. 12 (5), e0178315 (2017).</li><li>Nelson, R. W., Himsel, C. A., Feldman, E. C., Bottoms, G. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Glucose+tolerance+and+insulin+response+in+normal-weight+and+obese+cats.">Glucose tolerance and insulin response in normal-weight and obese cats.</a> Am J Vet Res. 51 (9), 1357-1362 (1990).</li><li>Staup, M., Aoyagi, G., Bayless, T., Wang, Y., Chng, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Characterization+of+Metabolic+Status+in+Nonhuman+Primates+with+the+Intravenous+Glucose+Tolerance+Test.">Characterization of Metabolic Status in Nonhuman Primates with the Intravenous Glucose Tolerance Test.</a> J Vis Exp. (117), e52895 (2016).</li><li>Hall, J. E., do Carmo, J. M., da Silva, A. A., Wang, Z., Hall, M. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Obesity-induced+hypertension:+interaction+of+neurohumoral+and+renal+mechanisms.">Obesity-induced hypertension: interaction of neurohumoral and renal mechanisms.</a> Circ Res. 116 (6), 991-1006 (2015).</li><li>Linz, D., Hohl, M., Mahfoud, F., Reil, J. C., Linz, W., H&#252;bschle, T., Juretschke, H. P., Neumann-H&#228;flin, C., R&#252;tten, H., B&#246;hm, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cardiac+remodeling+and+myocardial+dysfunction+in+obese+spontaneously+hypertensive+rats.">Cardiac remodeling and myocardial dysfunction in obese spontaneously hypertensive rats.</a> J Transl Med. 10 (10), 187 (2012).</li><li>Sasser, T. A., Chapman, S. E., Li, S., Hudson, C., Orton, S. P., Diener, J. M., Gammon, S. T., Correcher, C., Leevy, W. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Segmentation+and+measurement+of+fat+volumes+in+murine+obesity+models+using+X-ray+computed+tomography.">Segmentation and measurement of fat volumes in murine obesity models using X-ray computed tomography.</a> J Vis Exp. (62), e3680 (2012).</li><li>Kawai, T., Ito, T., Ohwada, K., Mera, Y., Matsushita, M., Tomoike, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hereditary+postprandial+hypertriglyceridemic+rabbit+exhibits+insulin+resistance+and+central+obesity:+a+novel+model+of+metabolic+syndrome.">Hereditary postprandial hypertriglyceridemic rabbit exhibits insulin resistance and central obesity: a novel model of metabolic syndrome.</a> Arterioscler Thromb Vasc Biol. 26 (12), 2752-2757 (2006).</li><li>Shiomi, M., Kobayashi, T., Kuniyoshi, N., Yamada, S., Ito, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Myocardial+infarction-prone+Watanabe+heritable+hyperlipidemic+rabbits+with+mesenteric+fat+accumulation+are+a+novel+animal+model+for+metabolic+syndrome.">Myocardial infarction-prone Watanabe heritable hyperlipidemic rabbits with mesenteric fat accumulation are a novel animal model for metabolic syndrome.</a> Pathobiology. 79 (6), 329-338 (2012).</li><li>Hildrum, B., Mykletun, A., Hole, T., Midthjell, K., Dahl, A. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Age-specific+prevalence+of+the+metabolic+syndrome+defined+by+the+International+Diabetes+Federation+and+the+National+Cholesterol+Education+Program:+The+Norwegian+HUNT+2+study.">Age-specific prevalence of the metabolic syndrome defined by the International Diabetes Federation and the National Cholesterol Education Program: The Norwegian HUNT 2 study.</a> BMC Public Health. 7, 220 (2007).</li></ol>

Etablering af en passende eksperimentel model kan give en mere konsistent og pålidelig metode til at studere udviklingen af MetS, og det er også nødvendigt at forstå de grundlæggende mekanismer, der ligger til grund for organer og systemer, remodeling. Her, vi beskriver metoderne til at udvikle en relevant eksperimentel model af kost-induceret MetS og hvordan man skal vurdere de vigtigste bestanddele i denne klynge af metaboliske og kardiovaskulære misdannelser, der karakteriserer denne model: central fedme, forhø...

Fedme og metaboliske syndrom (MetS) er blevet et voksende problem for folkesundheden og klinisk praksis, givet deres øget forekomst på grund af stigningen i stillesiddende livsstil og usunde spisevaner vaner1. Der er flere definitioner af MetS, men de fleste af dem beskrive det som en klynge af hjerte-kar- og metaboliske ændringer såsom abdominal fedme, nedsat HDL og forhøjet LDL-kolesterol, forhøjet triglycerider, glucose intolerance og hypertension2 ,3,4. Diagnosen kræver, at tre af disse fem kriterier er til stede.
På grund af dyremodeller har grundlæggende forskning kunnet undersøge de underliggende patologiske processer såsom MetS mekanismer. Flere dyremodeller har været brugt, men det er af afgørende betydning at model valg gengiver de vigtigste kliniske manifestationer af den menneskelige patologi (figur 1). Med dette formål, har dyremodeller betragtes som ligner mennesker, hovedsagelig hunde og svin, udviklet (Se Verkest5 og Zhang & Lerman6 til gennemsyn). Imidlertid viser canine modeller ikke alle komponenter i MetS, eftersom udviklingen af åreforkalkning eller hyperglykæmi i hunde gennem kosten er tvivlsom5. Svin modeller præsentere de mest anatomiske og fysiologiske lighed med mennesker, og dermed tilbyde betydelige intelligent strøm til belyse de underliggende MetS mekanismer, men deres vedligeholdelse og kompleksiteten af de eksperimentelle procedurer gør brug denne model meget arbejdskraft intensiv og bekostelig6.
På den anden side gnavere modeller (mus og rotter), kost-induceret spontan og transgene, har været flittigt brugt i litteratur for studiet af fedme, forhøjet blodtryk, og MetS og dens patologiske konsekvenser i forskellige organer og systemer (Se Wong et al. 7 til gennemsyn). Selv om brugen af disse modeller er mere overkommelig end hunde eller svin, har de vigtige ulemper. Faktisk, afhængigt af stammen, dyr udvikle nogle komponenter i MetS, mens andre såsom hypertension, hyperglykæmi, og hyperinsulinemia er fraværende7. Desuden en af de vigtigste komponenter i MetS, fedme, i nogle genetisk modificerede stammer, kun afhænger ikke af faktorer i forbindelse med kosten, snarere det har vist sig at nogle dyr bliver overvægtige med normale eller endda reduceret mad indtag8. Endelig, mus og rotter viser en naturlig mangel i cholesteryl ester overførsel protein (CETP) og bruge HDL som den store transportmiddel kolesterol, hvilket gør dem relativt resistente over for udviklingen af åreforkalkning. Dette er en vigtig forskel i lipid metabolisme med mennesker, der udtrykker CETP og transportere deres kolesterol hovedsagelig i LDL9.
Omvendt, laboratorium kanin repræsenterer et mellemstadium mellem de større dyr og gnaver eksperimentelle modeller. Således, kaninen kan nemt fremsendes til forskellige typer af protokoller med minimale krav af personale og vedligeholdelse, at være mere let håndteres i eksperimentelle procedurer end større dyremodeller. Endvidere, det er blevet rapporteret, at kaniner fodres med et højt fedtindhold kost har lignende hæmodynamiske og neurohumoral forandringer som fede mennesker8,10,11. Bemærk, om lipid metabolisme, kanin har rigelige CETP i plasma og deres lipoprotein profil er LDL-rige12, som er også ligner for mennesker. Derudover udvikle kaniner hyperlipidæmi ganske hurtigt idet som planteædere, de er meget følsomme over for kosten fedt13.
<img alt="Figure 1" class="xfigimg" src="/files/ftp_upload/57117/57117fig1.jpg"> Figur 1: sammenligning af MetS dyremodeller. Se Verkest5, Zhang og Lerman6og Wong et al. 7 til gennemsyn. "<img alt="Equation 1" src="/files/ftp_upload/57117/57117eq1.jpg">" angiver en fordel og "<img alt="Equation 2" src="/files/ftp_upload/57117/57117eq2.jpg">" angiver en ulempe. *kontroversielle, afhænger af undersøgelsen, *som pegede ud af Carroll et al. 8, nogle genetisk modificerede stammer blive fede uafhængigt af fødeindtagelse. CEPT: cholesteryl ester overførsel protein. GTT: glukose tolerance test. <a href="//cloudflare.jove.com/files/ftp_upload/57117/57117fig1large.jpg" target="_blank">Venligst klik her for at se en større version af dette tal.</a>
For at belyse de grundlæggende mekanismer de patologiske remodeling produceret af MetS i forskellige organer og systemer og opnå forståelse af dette komplekse patologi, valget af en eksperimentel model, der gengiver de vigtigste komponenter i menneskelige MetS er afgørende. Kaninen kan give mange fordele givet sin lighed med menneskets fysiologi og prisoverkommelighed af brug i kronisk protokoller og målinger. I denne linje, par kost-induceret kanin modeller ved hjælp af højt fedtindhold og højt-saccharose diet har været brugt14,15,16,17,18,19 (tabel 1), og en karakterisering af de forskellige komponenter i MetS er af stor betydning når vedrørende en fænotype med orgel remodellering. Således, denne artikel Hovedformålet er at beskrive metoderne for at udvikle en model for kost-induceret MetS i kaniner, der giver mulighed for undersøgelse af dens Patofysiologi og indvirkning i orgel remodellering.
<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always"><tbody>
<tr>
<td rowspan="2">Undersøgelse</td>
			<td rowspan="2">Kost</td>
			<td rowspan="2">Varighed</td>
			<td rowspan="2">Racen</td>
			<td colspan="4">MetS komponenter</td>
		</tr>
<tr>
<td>OB</td>
			<td>HT</td>
			<td>HG</td>
			<td>DL</td>
		</tr>
<tr>
<td rowspan="2">Yin et al. (2002)14</td>
			<td>·    10% fedt</td>
			<td rowspan="2">24 uger</td>
			<td>·      Mandlige NZW</td>
			<td rowspan="2"><img alt="Equation 2" src="/files/ftp_upload/57117/57117eq2.jpg"></td>
			<td rowspan="2">-</td>
			<td rowspan="2"><img alt="Equation 1" src="/files/ftp_upload/57117/57117eq1.jpg"></td>
			<td rowspan="2"><img alt="Equation 1" src="/files/ftp_upload/57117/57117eq1.jpg"></td>
		</tr>
<tr>
<td>·    37% saccharose</td>
			<td>·      2 kg</td>
		</tr>
<tr>
<td rowspan="2">Zhao et al. (2007)15</td>
			<td>·    10% fedt</td>
			<td rowspan="2">36 uger</td>
			<td>·      Mandlige JW</td>
			<td rowspan="2"><img alt="Equation 1" src="/files/ftp_upload/57117/57117eq1.jpg"></td>
			<td rowspan="2"><img alt="Equation 2" src="/files/ftp_upload/57117/57117eq2.jpg"></td>
			<td rowspan="2"><img alt="Equation 2" src="/files/ftp_upload/57117/57117eq2.jpg"></td>
			<td rowspan="2"><img alt="Equation 2" src="/files/ftp_upload/57117/57117eq2.jpg"></td>
		</tr>
<tr>
<td>·    30% saccharose</td>
			<td>·      16 uger</td>
		</tr>
<tr>
<td rowspan="3">Helfestein et al. (2011)16</td>
			<td>·    10% fedt</td>
			<td rowspan="3">24 uger</td>
			<td>·      Mandlige NZW</td>
			<td rowspan="3"><img alt="Equation 2" src="/files/ftp_upload/57117/57117eq2.jpg"></td>
			<td rowspan="3">-</td>
			<td rowspan="3"><img alt="Equation 1" src="/files/ftp_upload/57117/57117eq1.jpg"></td>
			<td rowspan="3"><img alt="Equation 1" src="/files/ftp_upload/57117/57117eq1.jpg"></td>
		</tr>
<tr>
<td>·    40% saccharose</td>
			<td>·      12 uger</td>
		</tr>
<tr>
<td>·    0,5-0,1 kolesterol</td>
			<td></td>
		</tr>
<tr>
<td rowspan="2">Ning et al. (2015)17</td>
			<td>·    10% fedt</td>
			<td rowspan="2">8-16 uger</td>
			<td>·      Mandlige WHHL</td>
			<td rowspan="2"><img alt="Equation 2" src="/files/ftp_upload/57117/57117eq2.jpg"></td>
			<td rowspan="2">-</td>
			<td rowspan="2"><img alt="Equation 2" src="/files/ftp_upload/57117/57117eq2.jpg"></td>
			<td rowspan="2"><img alt="Equation 1" src="/files/ftp_upload/57117/57117eq1.jpg"></td>
		</tr>
<tr>
<td>·    30% fructose *</td>
			<td>·      12 uger</td>
		</tr>
<tr>
<td rowspan="2">Liu et al. (2016)18</td>
			<td>·    10% fedt</td>
			<td rowspan="2">48 uger</td>
			<td>·      Mandlige NZW</td>
			<td rowspan="2"><img alt="Equation 2" src="/files/ftp_upload/57117/57117eq2.jpg"></td>
			<td rowspan="2">-</td>
			<td rowspan="2"><img alt="Equation 1" src="/files/ftp_upload/57117/57117eq1.jpg"></td>
			<td rowspan="2"><img alt="Equation 1" src="/files/ftp_upload/57117/57117eq1.jpg"></td>
		</tr>
<tr>
<td>·    30% saccharose</td>
			<td>·      12 uger</td>
		</tr>
<tr>
<td>Arias-Mutis et al. (2017)19</td>
			<td>·    15% fedt</td>
			<td>28 uger</td>
			<td>·      Mandlige NZW</td>
			<td><img alt="Equation 1" src="/files/ftp_upload/57117/57117eq1.jpg"></td>
			<td><img alt="Equation 1" src="/files/ftp_upload/57117/57117eq1.jpg"></td>
			<td><img alt="Equation 1" src="/files/ftp_upload/57117/57117eq1.jpg"></td>
			<td><img alt="Equation 1" src="/files/ftp_upload/57117/57117eq1.jpg"></td>
		</tr>
</tbody></table>Tabel 1: kost-induceret MetS kanin modeller med højt fedtindhold, high saccharose diet. Symbolet "<img alt="Equation 2" src="/files/ftp_upload/57117/57117eq2.jpg">"angiver fravær,"<img alt="Equation 1" src="/files/ftp_upload/57117/57117eq1.jpg">" tilstedeværelse, og "-" ikke evalueret. * begrænset. WHHL, Watanabe arvelige hiperlipidemic kaniner. JW, japansk hvide kaniner. OB, fedme. HT, hypertension. HG, hyperglykæmi. DL, dyslipidæmi.

<table>
	<tbody>
		<tr>
			<td>Veterinary scale</td>
			<td>SOEHNLE</td>
			<td>7858</td>
			<td>Scale 
			https://www.soehnle-professional.com/en/productgroup/details/103/veterinary-scale</td>
		</tr>
		<tr>
			<td>Shovel for aluminum feed</td>
			<td>COPELE</td>
			<td>10308</td>
			<td>Shovel for aluminum feed 
			http://copele.com/es/herramientas/48-pala-para-pienso-de-aluminio.html</td>
		</tr>
		<tr>
			<td>Balance</td>
			<td>PCE Ib&#233;rica</td>
			<td>PCE-TB 15</td>
			<td>Balance 
			http://www.pce-iberica.es/medidor-detalles-tecnicos/balanzas/balanza-compacta-pce-bdm.htm</td>
		</tr>
		<tr>
			<td>Strainer (20 cm diam.)</td>
			<td>ZWILLING</td>
			<td>39643-020-0</td>
			<td>Strainer 
			https://es.zwilling-shop.com/Menaje-del-hogar/Menaje-de-cocina/Menaje-especial/Accesorios/Colador-20-cm-ZWILLING-39643-020-0.html</td>
		</tr>
		<tr>
			<td>Bowl</td>
			<td>ZWILLING</td>
			<td>40850-751-0</td>
			<td>Scale 
			https://www.soehnle-professional.com/en/productgroup/details/103/veterinary-scale</td>
		</tr>
		<tr>
			<td>Funnel</td>
			<td>BT Ingenieros</td>
			<td>not available</td>
			<td>Funnel 
			http://www.bt-ingenieros.com/fluidos-y-combustibles/961-juego-de-4-embudos-de-plastico.html?gclid=EAIaIQobChMIuInui_y-1QIVASjTCh28Zwf-EAQYBSABEgK7xPD_BwE</td>
		</tr>
		<tr>
			<td>Introcan Certo 22G blue</td>
			<td>B Braun</td>
			<td>4251318</td>
			<td>Peripheral intravenous catheter 
			http://www.bbraun-vetcare.es/producto/introcan-</td>
		</tr>
		<tr>
			<td>Propofol Lipuro 10 mg/ml vial 20 ml</td>
			<td>B Braun</td>
			<td>3544761VET</td>
			<td>General intravenous anesthetic 
			http://www.bbraun-vetcare.es/producto/propofol-lipuro-1-</td>
		</tr>
		<tr>
			<td>FisioVet serum solution 500ml</td>
			<td>B Braun</td>
			<td>472779</td>
			<td>Scale 
			https://www.soehnle-professional.com/en/productgroup/details/103/veterinary-scale</td>
		</tr>
		<tr>
			<td>Askina Film Vet 1,25cm x 5m</td>
			<td>B Braun</td>
			<td>OCT13501</td>
			<td>Plastic Plaster 
			http://www.bbraun-vetcare.es/producto/askina-film-vet</td>
		</tr>
		<tr>
			<td>Askina Film Vet 2,50cm x 5m</td>
			<td>B Braun</td>
			<td>OCT13502</td>
			<td>Plastic Plaster 
			http://www.bbraun-vetcare.es/producto/askina-film-vet</td>
		</tr>
		<tr>
			<td>Injekt siringe 10ml luer</td>
			<td>B Braun</td>
			<td>4606108V</td>
			<td>Injection-aspiration syringe of two single-use bodies 
			http://www.bbraun-vetcare.es/producto/injekt-</td>
		</tr>
		<tr>
			<td>Seca 201</td>
			<td>seca</td>
			<td>seca 201</td>
			<td>Ergonomic tape for measuring perimeters 
			https://www.seca.com/es_es/productos/todos-los-productos/detalles-del-producto/seca201.html#referred</td>
		</tr>
		<tr>
			<td>Sterican 21Gx1&#34; - 0,8x25mm verde</td>
			<td>B Braun</td>
			<td>4657543</td>
			<td>Single Use Hypodermic Needle 
			http://www.bbraun-vetcare.es/producto/agujas-hipodermicas-sterican-</td>
		</tr>
		<tr>
			<td>CONTOURNEXT-Meter</td>
			<td>BAYER</td>
			<td>84413470</td>
			<td>Blood glucose analysis system 
			http://www.contournextstore.com/en/contour-next-meter-2</td>
		</tr>
		<tr>
			<td>CONTOUR NEXT test strips</td>
			<td>BAYER</td>
			<td>83624788</td>
			<td>Blood glucose test strips 
			http://www.contournextstore.com/en/contour-next-test-strips-100-ct-package</td>
		</tr>
		<tr>
			<td>MICROLET NEXT LANCING DEVICE</td>
			<td>BAYER</td>
			<td>6702</td>
			<td>Lancing device 
			http://www.contournextstore.com/en/new-microlet-next-lancing-device</td>
		</tr>
		<tr>
			<td>MICROLET 2 Colored Lancets</td>
			<td>BAYER</td>
			<td>81264857</td>
			<td>Ultra-thin sterile lancet for capillary puncture 
			http://www.contournextstore.com/en/microlet2-colored-lancets-100s</td>
		</tr>
		<tr>
			<td>Injekt 20ml luer siringe</td>
			<td>B Braun</td>
			<td>4606205V</td>
			<td>Scale 
			https://www.soehnle-professional.com/en/productgroup/details/103/veterinary-scale</td>
		</tr>
		<tr>
			<td>Askina Mullkompressen 7,5x7,5cm - sterile</td>
			<td>B Braun</td>
			<td>9031219N</td>
			<td>Sterile gauze packets in envelopes 
			http://www.bbraun-vetcare.es/producto/askina-mullkompressen-esteril</td>
		</tr>
		<tr>
			<td>Emla lidocaine/prilocaine</td>
			<td>AstraZeneca</td>
			<td>not available</td>
			<td>Local anesthetics 
			https://www.astrazeneca.es/areas-terapeuticas/neurociencias.html</td>
		</tr>
		<tr>
			<td>Introcan Certo 18G short</td>
			<td>B Braun</td>
			<td>4251342</td>
			<td>Peripheral intravenous catheter 
			http://www.bbraun-vetcare.es/producto/introcan-</td>
		</tr>
		<tr>
			<td>Introcan Certo 20G</td>
			<td>B Braun</td>
			<td>4251326</td>
			<td>Peripheral intravenous catheter 
			http://www.bbraun-vetcare.es/producto/introcan-</td>
		</tr>
		<tr>
			<td>Blood Pressure Transducers-MA1 72-4497</td>
			<td>Harvard Apparatus</td>
			<td>724497</td>
			<td>Transducer for monitoring blood pressure 
			http://www.harvardapparatus.com/physiology/physiological-measurements/transducers/pressure-transducers/research-grade-pressure-transducers.html</td>
		</tr>
		<tr>
			<td>PowerLab 2/26</td>
			<td>AD Instruments</td>
			<td>ML826</td>
			<td>Amplifier 
			https://www.adinstruments.com/products/powerlab</td>
		</tr>
		<tr>
			<td>LabChart ver. 6</td>
			<td>AD Instruments</td>
			<td>not available</td>
			<td>Acquisition software 
			https://www.adinstruments.com/products/labchart</td>
		</tr>
		<tr>
			<td>Animal Bio Amp</td>
			<td>AD Instruments</td>
			<td>FE136</td>
			<td>Amplifier 
			https://www.adinstruments.com/products/bio-amps#product-FE136</td>
		</tr>
		<tr>
			<td>K2EDTA 7.2mg</td>
			<td>BD</td>
			<td>367861</td>
			<td>Blood collection tubes 
			http://catalog.bd.com/nexus-ecat/getProductDetail?productId=367861</td>
		</tr>
		<tr>
			<td>Centrifuge</td>
			<td>SciQuip</td>
			<td>2-16KL</td>
			<td>Centrifuge 
			http://www.sigma-centrifuges.co.uk/store/products/refrigerated-sigma-2-16k-centrifuge/</td>
		</tr>
		<tr>
			<td>Eppendorf Reference 2, 100 &#8211; 1000 &#956;L</td>
			<td>Eppendorf</td>
			<td>4920000083</td>
			<td>Pipette 
			https://online-shop.eppendorf.es/ES-es/Pipeteo-44563/Pipetas-44564/Eppendorf-Reference2-PF-42806.html</td>
		</tr>
		<tr>
			<td>Eppendorf Safe-Lock Tubes, 0.5 mL</td>
			<td>Eppendorf</td>
			<td>30121023</td>
			<td>Tubes 
			https://online-shop.eppendorf.es/ES-es/Puntas-tubos-y-placas-44512/Tubos-44515/Eppendorf-Safe-Lock-Tubes-PF-8863.html</td>
		</tr>
		<tr>
			<td>NZW rabbits (16-18 weeks old)</td>
			<td>Granja San Bernardo</td>
			<td>not available</td>
			<td>New Zealand White rabbits 
			http://www.granjasanbernardo.com/en/welcome/</td>
		</tr>
		<tr>
			<td>Sucrose&#160;</td>
			<td>Sigma</td>
			<td>S0389-5KG</td>
			<td>Sucrose for drinking solution 
			http://www.sigmaaldrich.com/catalog/product/sigma/s0389?lang=es&#38;region=ES</td>
		</tr>
		<tr>
			<td>Rabbit maintenance control diet</td>
			<td>Ssniff</td>
			<td>V2333-000</td>
			<td>Control diet 
			http://www.ssniff.com/</td>
		</tr>
		<tr>
			<td>Rabbit high-fat diet</td>
			<td>Ssniff</td>
			<td>S9052-E020</td>
			<td>High-fat diet 
			http://www.ssniff.com/</td>
		</tr>
		<tr>
			<td>Rabbit rack and drinker</td>
			<td>Sodispan</td>
			<td>not available</td>
			<td>Rack for rabbits 
			https://www.sodispan.com/jaulas-y-racks/racks-conejo-y-cobaya/</td>
		</tr>
		<tr>
			<td>Rabbit restrainer</td>
			<td>Zoonlab</td>
			<td>3045601</td>
			<td>http://www.zoonlab.de/en/index.html</td>
		</tr>
	</tbody>
</table>

an experimental model of diet-induced metabolic syndrome in rabbit: methodological considerations, development, and assessment

I de seneste år, er fedme og metaboliske syndrom (MetS) blevet et voksende problem for folkesundheden og klinisk praksis, givet deres øget forekomst på grund af stigningen i stillesiddende livsstil og usunde spisevaner. Takket være dyremodeller, kan grundlæggende forskning undersøge de underliggende patologiske processer såsom MetS mekanismer. Her, beskriver vi metoderne til at udvikle en eksperimentel kanin model af kost-induceret MetS og sin vurdering. Efter en periode med akklimatisering fodres dyrene en højt fedtindhold (10% hydrogenerede kokosolie og 5% fedt), høj-saccharose (15% saccharose opløst i vand) kost til 28 uger. I denne periode flere eksperimentelle procedurer blev udført for at vurdere de forskellige komponenter i MetS: morfologiske og blodtryksmålinger, glucose tolerance bestemmelse og analyse af flere plasma markører. Ved udgangen af forsøgsperioden, dyr udviklet centrale fedme, mild hypertension, præ-diabetes og dyslipidæmi med lavt HDL og høj LDL forhøjelse af triglycerid (TG), gengiver således hovedkomponenterne i menneskelige MetS. Denne kroniske model giver nye perspektiver for at forstå de underliggende mekanismer i progression af sygdommen, påvisning af prækliniske og kliniske markører, der tillader identifikation af patienter i fare, eller endda afprøvning af nye terapeutiske tilgange til behandling af denne komplekse patologi.

MetS repræsenterer en klynge af metaboliske og kardiovaskulære misdannelser hvis undersøgelse kan lettes ved hjælp af eksperimentelle modeller. Faktisk, for at belyse de underliggende den patologiske remodeling produceret af MetS mekanismer, valget af en eksperimentel model, der passende ligner den menneskelige tilstand og er velegnet til forskning er af afgørende betydning. Vi præsenterer her, metoder til at fremkalde MetS i kanin ved hjælp af en kost med højt indhold af mættet ...

Watch this Scientific Journal Video about An Experimental Model of Diet-Induced Metabolic Syndrome in Rabbit: Methodological Considerations, Development, and Assessment at JoVE.com

An Experimental Model of Diet-Induced Metabolic Syndrome in Rabbit: Methodological Considerations, Development, and Assessment

En eksperimentel Model af kost-induceret metabolisk syndrom i kanin: metodiske overvejelser, udvikling og vurdering

Vi beskriver metoder til at udvikle en eksperimentel model af kost-induceret metabolisk syndrom (MetS) i kaniner ved hjælp af en højt fedtindhold, high saccharose diet. Dyr udviklet centrale fedme, mild hypertension, præ-diabetes og dyslipidæmi, dermed gengive de vigtigste komponenter i menneskelige MetS. Denne kroniske model giver mulighed for erhvervelse af viden underliggende mekanismer for sygdomsprogression.

In recent years, obesity and metabolic syndrome (MetS) have become a growing problem for public health and clinical practice, given their increased ...

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