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DOI :
10.3791/57134-v
•
8:54 min
March 9th, 2018
Chapters
0:05
Title
0:36
Day 1: Plasmolyze Tuber Tissue
1:40
Days 2 and 3: Generate Potato Protoplasts and Harvest and Wash Protoplasts
3:42
Day 3: Prepare Samples for Flow Cytometry
5:07
Day 3: Flow Cytometry of Potato Tuber Nuclei
6:31
Results: Endoreduplication of Isolated Tuber Protoplasts
8:07
Conclusion
本文描述的协议是一种测量马铃薯块茎内 endoreduplication 的方法 (马铃薯)。它包括质壁分离和原生质体提取步骤, 以减少下游流细胞分析中的噪声和碎片。
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