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DOI :
10.3791/57134-v
•
8:54 min
March 9th, 2018
Chapters
0:05
Title
0:36
Day 1: Plasmolyze Tuber Tissue
1:40
Days 2 and 3: Generate Potato Protoplasts and Harvest and Wash Protoplasts
3:42
Day 3: Prepare Samples for Flow Cytometry
5:07
Day 3: Flow Cytometry of Potato Tuber Nuclei
6:31
Results: Endoreduplication of Isolated Tuber Protoplasts
8:07
Conclusion
記載プロトコルは、じゃがいも (ジャガイモ) の塊茎内の表現を測定するための方法です。ノイズや下流フローサイトメトリーによる解析の残骸を減らすため原形質分離と原形質体の抽出手順が含まれています。
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