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DOI :
10.3791/57140-v
June 1st, 2018
Chapters
0:04
Title
0:44
Cell Culture
3:13
Lysis and Shearing
4:14
Hybridization Capture
6:14
Evaluation of Capture Yield by qPCR with Primers for the Target Capture Region of Interest
7:12
Results: Hybridization Capture of Chromatin-associated Proteins in Mammalian Cells
8:38
Conclusion
これ以降のプロテオーム解析の架橋クロマチンのシーケンス固有のキャプチャに依存する特定のターゲット遺伝子の新規 DNA と相互作用する蛋白質を識別する方法です。潜在的な結合蛋白質、またセル変更についての事前知識も必要ありません。技術は、当初は酵母の開発、哺乳類細胞に対して適応を今されています。
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