Name: an in vivo assessment of blood-brain barrier disruption in a rat model of ischemic stroke
Uploaded: 2018-03-11T14:00:00
Description: Watch this Scientific Journal Video about An In Vivo Assessment of Blood-Brain Barrier Disruption in a Rat Model of Ischemic Stroke at JoVE.com

著者らは、財政支援のアルダビール大学医療科学 (アイルランド) の研究のための副首相に感謝している (許可 No: 9607)。

動物実験を行い、アルダビール大学医療科学研究評議会のガイドラインに従ってすべてのプロシージャを行った (倫理的な ID 番号: 赤外線ARUMS。REC.1394.08). この可視化の研究で牧草研究所 (イラン ・ テヘラン) から得られる男性の大人の Sprague-dawley ラット (300-350 g) を使用しています。
1. 麻酔および血流計
<ol><li>4% イソフルランを使用して麻酔し、手術の間に亜酸化窒素 ...

<ol>
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A., Bohlooli, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Enalapril+attenuates+ischaemic+brain+oedema+and+protects+the+blood-brain+barrier+in+rats+via+an+anti-oxidant+action.">Enalapril attenuates ischaemic brain oedema and protects the blood-brain barrier in rats via an anti-oxidant action.</a> Clin Exp Pharmacol Physiol. 41 (3), 220-226 (2014).</li><li>Panahpour, H., Nekooeian, A. A., Dehghani, G. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Blockade+of+Central+Angiotensin+II+AT1+Receptor+Protects+the+Brain+from+Ischemia/Reperfusion+Injury+in+Normotensive+Rats.">Blockade of Central Angiotensin II AT1 Receptor Protects the Brain from Ischemia/Reperfusion Injury in Normotensive Rats.</a> Iran J Med Sci. 39 (6), 536-542 (2014).</li><li>Panahpour, H., Nekooeian, A. A., Dehghani, G. 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これまで、放射線と放射性トレーサー24,25, 蛍光顕微鏡26,27, EB 血管外漏出テクニック20,の検出などのさまざまな方法23血液脳関門の損傷を評価するために使用されています。EB 色素が強く血清アルブミンと結合することができると血管漏出を検出し、BBB 内訳<sup ...

血液脳関門 (BBB) 破壊による分子病態脳浮腫には、虚血性脳卒中の重要な合併症と脳卒中患者1,2の生存率の主要な決定要因が残っています。脳毛細血管内皮細胞 (BCECs) によって形成される、異なる神経血管コンポーネント (BCECs、ペリサイト、グリア、および神経細胞3間タイトジャンクショなど) で構成される、血液-脳関門 (BBB) を提供します、中枢神経系 (CNS)、末梢血循環4,5間の専門的かつ動的なインタ フェース。虚血再灌流傷害など侮辱でした BBB の機能の整合性を混乱させるし、最終的に原発性脳腫瘍の怪我や脳の炎症を引き起こす脳実質に白血球の循環のそれに続く侵入につながる6,7. 脳卒中の発生に続く BBB の機能不全の正確な検出には動物モデルが必要です。このようなモデルは、非常に重要な病態生理学的メカニズムの基礎となる、新しい神経保護戦略を導入することを勉強するためです。BBB の細胞培養技術を用いたモデル体外は高い開発し、BBB 生理病理学8,9,10の分子生物学研究に使用されます。それにもかかわらず、生体内で動物モデル、人間の臨床条件に類似の BBB の虚血性損傷を作り出す、この点で非常に価値があるも。エバンス ブルー (EB) の血管外漏出の定量的検出は BBB の整合性と神経変性疾患、虚血性脳卒中11,を含む機能の評価のために使用されているよく受け入れられ、敏感な技術12,13,14します。 この方法は、実験室でコスト効果の高い、可能、再現性のある、完全に該当します。その実装では、放射性トレーサー15や磁気共鳴画像 (MRI)16、他の方法のための前提条件となるなど、高度な機器は必要ありません。この記事で包括的に虚血性脳卒中のモデルラットによる EB 血管外漏出 bbb ランク評価の基本的な技術的なプロセスを示しています。

<table border="0" cellpadding="0" cellspacing="0" width="829">
	<tbody>
		<tr height="22">
			<td height="22" style="height:22px;width:208px;">Isoflurane</td>
			<td style="width:203px;">Piramal</td>
			<td style="width:225px;">AWN 34041100</td>
			<td style="width:193px;">20 - 25 &#176;C</td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;">2,3,5-Triphenyltetrazolium chloride (TTC)</td>
			<td>Molekula</td>
			<td>31216368</td>
			<td>4 years</td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;">Sprague&#8211;Dawley rats&#160;</td>
			<td>Pasture Institute (Tehran, Iran)</td>
			<td></td>
			<td>300-350g</td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;">Evans Blue&#160;</td>
			<td>Sigma-Aldrich&#160;</td>
			<td>314-13-6</td>
			<td></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;">Trichloroacetic acid&#160;</td>
			<td>Sigma-Aldrich&#160;</td>
			<td>76-03-9</td>
			<td>2 years</td>
		</tr>
		<tr height="23">
			<td height="23" style="height:23px;">Bupivacaine HCl (0.5%)</td>
			<td>Delpharm Tours</td>
			<td></td>
			<td>below&#160; 25 &#176;C</td>
		</tr>
		<tr height="23">
			<td height="23" style="height:23px;">Bupernorphine</td>
			<td>Exir (Iran)</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="23">
			<td height="23" style="height:23px;">Sodium Carbonate</td>
			<td>Sigma-Aldrich&#160;</td>
			<td>497-19-8</td>
			<td></td>
		</tr>
		<tr height="23">
			<td height="23" style="height:23px;">Sodium chloride&#160;</td>
			<td>Sigma-Aldrich&#160;</td>
			<td>7647-14-5</td>
			<td></td>
		</tr>
		<tr height="23">
			<td height="23" style="height:23px;">Di- Sodium hydrogen phosphate</td>
			<td>EMD Millipore&#160;</td>
			<td>231-448-7</td>
			<td></td>
		</tr>
		<tr height="23">
			<td height="23" style="height:23px;">Potassium chloride</td>
			<td>Sigma-Aldrich&#160;</td>
			<td>&#160;7447-40-7</td>
			<td></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:208px;">Ethanol&#160;</td>
			<td>Sigma-Aldrich&#160;</td>
			<td>64-17-5</td>
			<td></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;">silicone(Xantopren)</td>
			<td>Heraeus</td>
			<td>EN ISO 4823</td>
			<td></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;">Activator universal plus</td>
			<td>Heraeus</td>
			<td>66037445</td>
			<td></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;">Micro-Dissecting forceps</td>
			<td>Stoelting</td>
			<td>52100-41</td>
			<td></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;">Spring Scisors</td>
			<td>Stoelting</td>
			<td>52130-00</td>
			<td></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;">Operating&#160; Scissors</td>
			<td>Roboz</td>
			<td>52140-70</td>
			<td></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;">Brain matrix&#160;</td>
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			<td>51390</td>
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</table>

an in vivo assessment of blood-brain barrier disruption in a rat model of ischemic stroke

虚血性脳卒中は、分子病態脳浮腫、血液脳関門 (BBB) の破壊を介するその後原発性脳損傷につながります。誘発虚血性脳卒中ラットは確立され、BBB の機能の整合性を調査するため生体内でモデルとして使用されます。エバンス ブルー (EB) の虚血性損傷脳のサンプルの吸光光度検出は研究と新規治療戦略の開発のための信頼性の高い正当化を提供すること。このメソッドは、再現性のある結果を生成し、特別な装置を必要としない任意の研究室では、適用。ラットにおける虚血性脳卒中の誘導 EB の血管外漏出の検出に関する可視化および技術的なガイドラインを紹介します。

Sham ラットの左半球と右半球の EB レベルに差はありませんでした (1.06 ± 0.1 μ g/g と 1.1 ± 0.09 μ g/g、それぞれ)。図 2A 2B、一過性虚血の誘導に示すように (90 分虚血/24 h 再灌流) 理事会レベルの差の原因 (10.41 ± 0.84 μ g/g, p < 0.001) と比較して、それぞれの虚血ラットの左半球のsham ラットの半球。総称して、これらの調査結果を示し通常の条件下で EB が...

Watch this Scientific Journal Video about An In Vivo Assessment of Blood-Brain Barrier Disruption in a Rat Model of Ischemic Stroke at JoVE.com

An In Vivo Assessment of Blood-Brain Barrier Disruption in a Rat Model of Ischemic Stroke

虚血性脳卒中のモデルラットの血液脳関門の破壊のIn Vivo評価

この手順の全体的な目標は、虚血性脳卒中のモデルラットの血液脳関門の破壊の生体内評価の再現性の高い技術を提供することです。

Ischemic stroke leads to vasogenic cerebral edema and subsequent primary brain injury, which is mediated through destruction of the blood-brain barrier ...

Research

JoVE Journal

Neuroscience

An In Vivo Assessment of Blood-Brain Barrier Disruption in a Rat Model of Ischemic Stroke

Generation of an Immortalized Murine Brain Microvascular Endothelial Cell Line as an In Vitro Blood Brain Barrier Model

Generation of an Immortalized Murine Brain Microvascular Endothelial Cell Line as an In Vitro Blood Brain Barrier Model

として不死化マウス脳微小血管内皮細胞株の生成<em&gt; in vitroで</em&gt;血液脳関門モデル

Epithelial and endothelial cells (EC) are building paracellular barriers which protect the tissue from the external and internal environment. The ...

Functional Neuroimaging Using Ultrasonic Blood-brain Barrier Disruption and Manganese-enhanced MRI

超音波血液脳関門の破壊とマンガン増強MRIを用いた脳機能イメージングの試み

Although mice are the dominant model system for studying the genetic and molecular underpinnings of neuroscience, functional neuroimaging in mice remains ...

Assessment of Blood-brain Barrier Permeability by Intravenous Infusion of FITC-labeled Albumin in a Mouse Model of Neurodegenerative Disease

神経変性疾患のマウスモデルで FITC 標識アルブミンの点滴静注による血液脳関門透過性の評価

Disruption of blood-brain barrier (BBB) integrity is a common feature for different neurological and neurodegenerative diseases. Although the interplay ...

An In Vivo Blood-brain Barrier Permeability Assay in Mice Using Fluorescently Labeled Tracers

An In Vivo Blood-brain Barrier Permeability Assay in Mice Using Fluorescently Labeled Tracers

生体内で血液脳関門透過性アッセイを用いたマウス蛍光トレーサーに分類

Blood-brain barrier (BBB) is a specialized barrier that protects the brain microenvironment from toxins and pathogens in the circulation and maintains ...

A Preclinical Model to Assess Brain Recovery After Acute Stroke in Rats

ラットの急性脳卒中後の脳回復を評価する前臨床モデル

The purpose of this study was to establish and validate an animal brain ischemia model in the recovery and sequela stages. A middle cerebral artery ...

A Benchtop Approach to the Location Specific Blood Brain Barrier Opening using Focused Ultrasound in a Rat Model

ラットモデルにおける集中超音波を用いた位置特異的血液脳関門開口へのベンチトップアプローチ

Stereotaxic surgery is the gold standard for localized drug and gene delivery to the rodent brain. This technique has many advantages over systemic ...

Measuring Post-Stroke Cerebral Edema, Infarct Zone and Blood-Brain Barrier Breakdown in a Single Set of Rodent Brain Samples

げっ歯類の脳サンプルの単一のセットで脳卒中後脳浮腫、梗塞ゾーンおよび血液脳関門破壊を測定する

One of the most common causes of morbidity and mortality worldwide is ischemic stroke. Historically, an animal model used to stimulate ischemic stroke ...

A Rat Model of EcoHIV Brain Infection

エコHIV脳感染症のラットモデル

It has been well studied that the EcoHIV infected mouse model is of significant utility in investigating HIV associated neurological complications. ...

Induction and Assessment of Levodopa-induced Dyskinesias in a Rat Model of Parkinson's Disease

パーキンソン病のラットモデルにおけるレボドパ誘発性ジスキネジアの誘導と評価

Levodopa (L-DOPA) remains the gold-standard therapy used to treat Parkinson&#39;s disease (PD) motor symptoms. However, unwanted involuntary movements ...

Transendothelial Resistance Measurement to Assess Cell Barrier Integrity

Barrier Functional Integrity Recording on bEnd.3 Vascular Endothelial Cells via Transendothelial Electrical Resistance Detection

Applications of TEER Detection to Assess Cell Barrier Integrity (Video) | JoVE

経内皮電気抵抗検出 による bEnd.3血管内皮細胞のバリア機能完全性記録

The blood-brain barrier (BBB) is a dynamic physiological structure composed of microvascular endothelial cells, astrocytes, and pericytes. By coordinating ...