Name: an in vivo assessment of blood-brain barrier disruption in a rat model of ischemic stroke
Uploaded: 2018-03-11T14:00:00
Description: Watch this Scientific Journal Video about An In Vivo Assessment of Blood-Brain Barrier Disruption in a Rat Model of Ischemic Stroke at JoVE.com

저자는 재정 지원에 대 한 아르다 빌 대학 의료 과학 (아르다 빌, 이란)의 연구에 대 한 부 장관에 게 감사 (No를 부여: 9607).

모든 절차는 동물 연구를 실시 위한 아르다 빌 대학교 의료 과학 연구 위원회의 지침에 따라 수행한 (윤리적 ID 번호: 적외선 ARUMS입니다. REC.1394.08).이 시각된 연구에서 사용 하는 성인 남성 Sprague-Dawley 쥐 (300-350 g) 목초지 연구소 (테헤란, 이란)에서 얻은.
1. 마 취와 Flowmetry
<ol><li>4 %isoflurane 사용 하 여 마 취를 유도 하 고 수술 하는 동안 질소 산화물 (70 %v / v)와 산소 (30 %v / v)의 ...

<ol>
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A., Bohlooli, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Enalapril+attenuates+ischaemic+brain+oedema+and+protects+the+blood-brain+barrier+in+rats+via+an+anti-oxidant+action.">Enalapril attenuates ischaemic brain oedema and protects the blood-brain barrier in rats via an anti-oxidant action.</a> Clin Exp Pharmacol Physiol. 41 (3), 220-226 (2014).</li><li>Panahpour, H., Nekooeian, A. A., Dehghani, G. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Blockade+of+Central+Angiotensin+II+AT1+Receptor+Protects+the+Brain+from+Ischemia/Reperfusion+Injury+in+Normotensive+Rats.">Blockade of Central Angiotensin II AT1 Receptor Protects the Brain from Ischemia/Reperfusion Injury in Normotensive Rats.</a> Iran J Med Sci. 39 (6), 536-542 (2014).</li><li>Panahpour, H., Nekooeian, A. A., Dehghani, G. 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지금까지, autoradiography 방사성 추적기24,25, 면역 형광 검사 현미경 검사 법26,27, EB 넘쳐 흐름 기술은20, 의 검출 등 다양 한 방법 23 혈액-뇌 장벽 손상을 평가 하는 데 사용 되었습니다. EB 염료 강력 하 게 혈 청 알 부 민에 바인딩할 수 이며 혈관 누설을 감지 하 고 측?...

Vasogenic 뇌 부 종 혈액-뇌 장벽 (BBB) 중단으로 인해 허 혈 성 뇌졸중의 중요 한 합병증 및 뇌졸중 환자1,2에서 생존 율의 주요 결정 요인이 남아 있다. 혈액-뇌 장벽 (BBB)는 뇌 모 세관 내 피 세포 (BCECs)에 의해 형성 된 뚜렷한 혈관 구성 요소 (예: BCECs, pericytes, astroglial, 및 신경 세포3중 꽉 접합)의 구성, 제공 하는 중앙 신경 조직 (CNS) 말 초 혈액 순환4,5사이의 특수 하 고 역동적인 인터페이스. 국 소 빈 혈 reperfusion 상해 등 모욕 BBB의 기능적 무결성을 방해 하 고 궁극적으로 트리거 대뇌 염증 및 기본 뇌 손상 뇌 실질에 순환 하는 백혈구의 침투를 후속으로 이어질 수 있습니다. 6 , 7. 뇌졸중의 발생에 따라 BBB의 부전의 정확한 검출을 위한 동물 모델 필요. 이러한 모델은 새로운 신경 보호 전략을 소개 하 고 기본 병 태 생리 기계 장치 공부에 대 한 매우 중요. 체 외에서 세포 문화 기반 모델 BBB의 높은 개발 하 고 되었습니다 BBB physiopathology8,,910의 분자 연구에 사용. 그럼에도 불구 하 고, vivo에서 동물 모델, 인간의 임상 조건에 비슷한 BBB의 허 혈 성 손상, 생산은 또한 매우 보람 이와. 에반스 블루 (EB)의 넘쳐 흐름의 정량적 검출은 잘 허용 하 고 민감한 기술 평가 BBB 무결성 및 신경 퇴행 성 질환, 허 혈 성 뇌졸중11, 를 포함 하 여 기능에 대 한 사용 되었습니다. 12 , 13 , 14.이 방법은 비용, 재현성, 및 완전히 적용 가능한 모든 실험 실험실에서. 그것의 구현 방사성 트레이 서15 또는 자기 공명 영상 (MRI)16다른 방법에 대 한 필수 구성 요소를 같은 고급 장비를 필요 하지 않습니다. 이 문서에서는, BBB 평가 EB 넘쳐 흐름을 사용 하 여 허 혈 성 뇌졸중의 쥐 모델에서의 기본적인 기술적 프로세스 포괄적으로 설명 합니다.

<table border="0" cellpadding="0" cellspacing="0" width="829">
	<tbody>
		<tr height="22">
			<td height="22" style="height:22px;width:208px;">Isoflurane</td>
			<td style="width:203px;">Piramal</td>
			<td style="width:225px;">AWN 34041100</td>
			<td style="width:193px;">20 - 25 &#176;C</td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;">2,3,5-Triphenyltetrazolium chloride (TTC)</td>
			<td>Molekula</td>
			<td>31216368</td>
			<td>4 years</td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;">Sprague&#8211;Dawley rats&#160;</td>
			<td>Pasture Institute (Tehran, Iran)</td>
			<td></td>
			<td>300-350g</td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;">Evans Blue&#160;</td>
			<td>Sigma-Aldrich&#160;</td>
			<td>314-13-6</td>
			<td></td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;">Trichloroacetic acid&#160;</td>
			<td>Sigma-Aldrich&#160;</td>
			<td>76-03-9</td>
			<td>2 years</td>
		</tr>
		<tr height="23">
			<td height="23" style="height:23px;">Bupivacaine HCl (0.5%)</td>
			<td>Delpharm Tours</td>
			<td></td>
			<td>below&#160; 25 &#176;C</td>
		</tr>
		<tr height="23">
			<td height="23" style="height:23px;">Bupernorphine</td>
			<td>Exir (Iran)</td>
			<td></td>
			<td></td>
		</tr>
		<tr height="23">
			<td height="23" style="height:23px;">Sodium Carbonate</td>
			<td>Sigma-Aldrich&#160;</td>
			<td>497-19-8</td>
			<td></td>
		</tr>
		<tr height="23">
			<td height="23" style="height:23px;">Sodium chloride&#160;</td>
			<td>Sigma-Aldrich&#160;</td>
			<td>7647-14-5</td>
			<td></td>
		</tr>
		<tr height="23">
			<td height="23" style="height:23px;">Di- Sodium hydrogen phosphate</td>
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an in vivo assessment of blood-brain barrier disruption in a rat model of ischemic stroke

허 혈 성 뇌졸중 vasogenic 대뇌 부 종 및 후속 기본 뇌 손상, 혈액-뇌 장벽 (BBB)의 파괴를 통해 중재 된다 이끌어 낸다. 유발 허 혈 성 뇌졸중 쥐 설립 되었고 BBB의 기능 무결성을 조사 하 비보에 모델로 사용. 허 혈 성 상해 두뇌 샘플에서 에반스 블루 (EB)의 spectrophotometric 감지 소설 치료 modalities의 개발과 연구에 대 한 신뢰할 수 있는 정당성을 제공할 수 있습니다. 이 방법은 재현성 결과 생성 하 고 특별 한 장비를 위한 필요 없이 어떤 실험실에 적용 됩니다. 여기, 선물이 쥐에서 허 혈 성 뇌졸중의 유도 따라 EB의 넘쳐 흐름의 검출에 시각 및 기술 지침.

가짜 운영 쥐의 좌 반구와 우 반구에서 EB 수준에 중요 한 차이가 없었다 (1.06 ± 0.1 µ g/g과 1.1 ± 0.09 µ g/g, 각각). 그림 2A 2B, 일시적인 허 혈의 유도에서 같이 (90 분 허 혈 / 24 h reperfusion) EB 수준에 상당한 차이 발생 (10.41 ± 0.84 µ g/g, p < 0.001) 각각에 비해 허 혈 성 쥐의 왼쪽된 반구에 운영 하는 가짜 쥐에 반구입니다. 샨 다, 이러한 결과 나타냅니다 정상적?...

Watch this Scientific Journal Video about An In Vivo Assessment of Blood-Brain Barrier Disruption in a Rat Model of Ischemic Stroke at JoVE.com

An In Vivo Assessment of Blood-Brain Barrier Disruption in a Rat Model of Ischemic Stroke

허 혈 성 뇌졸중의 쥐 모델에서 뇌혈관 장애의 비보에 평가

이 절차의 전반적인 목표는 비보에 허 혈 성 뇌졸중의 쥐 모델에서 뇌혈관 장애의 평가 대 한 높은 재현 기법을 제공 하는입니다.

Ischemic stroke leads to vasogenic cerebral edema and subsequent primary brain injury, which is mediated through destruction of the blood-brain barrier ...

Research

JoVE Journal

Neuroscience

An In Vivo Assessment of Blood-Brain Barrier Disruption in a Rat Model of Ischemic Stroke

Generation of an Immortalized Murine Brain Microvascular Endothelial Cell Line as an In Vitro Blood Brain Barrier Model

Generation of an Immortalized Murine Brain Microvascular Endothelial Cell Line as an In Vitro Blood Brain Barrier Model

로 불후의 손쥐 뇌 Microvascular 내피 세포 라인의 생성<em&gt; 체외에서</em&gt; 혈액 뇌 장벽 모델

Epithelial and endothelial cells (EC) are building paracellular barriers which protect the tissue from the external and internal environment. The ...

연구

신경과학

Functional Neuroimaging Using Ultrasonic Blood-brain Barrier Disruption and Manganese-enhanced MRI

초음파 혈액 - 뇌 장벽 파괴 및 망간 향상된 MRI를 사용하여 기능성 Neuroimaging

Although mice are the dominant model system for studying the genetic and molecular underpinnings of neuroscience, functional neuroimaging in mice remains ...

Assessment of Blood-brain Barrier Permeability by Intravenous Infusion of FITC-labeled Albumin in a Mouse Model of Neurodegenerative Disease

FITC 표시 된 알 부 민 신경 질환의 마우스 모델에서의 정 맥 주입에 의해 혈액-뇌 장벽 침투성의 평가

Disruption of blood-brain barrier (BBB) integrity is a common feature for different neurological and neurodegenerative diseases. Although the interplay ...

An In Vivo Blood-brain Barrier Permeability Assay in Mice Using Fluorescently Labeled Tracers

An In Vivo Blood-brain Barrier Permeability Assay in Mice Using Fluorescently Labeled Tracers

Vivo에서 혈액-뇌 장벽 침투성 분석 결과 사용 하 여 마우스에 붙일 추적기 표시

Blood-brain barrier (BBB) is a specialized barrier that protects the brain microenvironment from toxins and pathogens in the circulation and maintains ...

A Preclinical Model to Assess Brain Recovery After Acute Stroke in Rats

쥐에서 급성 뇌졸중 후 뇌 회복을 평가하는 전임상 모델

The purpose of this study was to establish and validate an animal brain ischemia model in the recovery and sequela stages. A middle cerebral artery ...

A Benchtop Approach to the Location Specific Blood Brain Barrier Opening using Focused Ultrasound in a Rat Model

쥐 모델에서 집중 초음파를 사용하여 위치 특정 혈액 뇌 장벽 개방에 대한 벤치 탑 접근

Stereotaxic surgery is the gold standard for localized drug and gene delivery to the rodent brain. This technique has many advantages over systemic ...

Measuring Post-Stroke Cerebral Edema, Infarct Zone and Blood-Brain Barrier Breakdown in a Single Set of Rodent Brain Samples

설치류 뇌 샘플의 단일 세트에서 뇌졸중 후 뇌 부종, 경색 영역 및 혈액 뇌 장벽 고장 측정

One of the most common causes of morbidity and mortality worldwide is ischemic stroke. Historically, an animal model used to stimulate ischemic stroke ...

A Rat Model of EcoHIV Brain Infection

에코HIV 뇌 감염의 쥐 모델

It has been well studied that the EcoHIV infected mouse model is of significant utility in investigating HIV associated neurological complications. ...

Induction and Assessment of Levodopa-induced Dyskinesias in a Rat Model of Parkinson's Disease

파킨슨 병의 쥐 모델에서 레보도파 유도 운동 장애의 유도 및 평가

Levodopa (L-DOPA) remains the gold-standard therapy used to treat Parkinson&#39;s disease (PD) motor symptoms. However, unwanted involuntary movements ...

Transendothelial Resistance Measurement to Assess Cell Barrier Integrity

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경내피 전기 저항 검출을 통한 bEnd.3 혈관 내피 세포의 장벽 기능 무결성 기록

The blood-brain barrier (BBB) is a dynamic physiological structure composed of microvascular endothelial cells, astrocytes, and pericytes. By coordinating ...