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DOI :
10.3791/57177-v
March 20th, 2018
Chapters
0:04
Title
2:01
Incubation of Carcinogen and Drugs with DNA in the Presence of Enzymatic Systems
3:04
Isolation of the DNA from the Incubate
4:30
32P-postlabeling for Detection of DNA Adduct Formation
5:04
TLC Separation of Adducted Deoxyoligonucleotides
5:43
Results: Study of the Potency of Metabolically Active Ellipticine Drug in Generating Covalent DNA Adducts
7:33
Conclusion
環境化学物質、酵素中間体の共有結合 DNA の生成に bioactivated が付加体に薬剤の効力を評価するがんとその治療法の開発に重要な分野です。DNA 付加体、その検出と定量の技術だけでなく、フォームに化合物の活性化のための方法を説明します。
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