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DOI :
10.3791/57209-v
•
12:49 min
April 4th, 2018
Chapters
0:04
Title
1:18
Quantitatively Acetylate and/or Succinylate Proteins Using Isotope-labeled Acetic or Succinic Anhydride
3:48
Digest Reacted Protein Samples Using Glu-C Endoproteinase
6:27
Fractionate Peptides Using Offline Basic-pH Reversed Phase HPLC
8:15
Example Data Analysis Tutorial
10:28
Results: Succinylation Stoichiometry Ratio Calculation of Pre-succinylated BSA
11:13
Conclusion
在这里, 我们通过对在定量之后引入的修改的比例分析, 对整个蛋白质组的站点特异性蛋白质乙酰化和/或琥珀酰化占用 (化学计量) 进行无偏量化。用稳定同位素标记的酸酐化学酰化反应。结合敏感数据无关的采集质谱, 得到了准确的场地占用量测量。
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