Name: transplantation of adipose tissue-derived stem cell sheet to reduce leakage after partial colectomy in a rat model
Uploaded: 2018-08-11T12:30:00
Description: Watch this Scientific Journal Video about Transplantation of Adipose Tissue-Derived Stem Cell Sheet to Reduce Leakage After Partial Colectomy in A Rat Model at JoVE.com

Gli autori sono grati al Dr. s.e.r. Hovius, Dr. M.A.M. Mureau e tutti i chirurghi del reparto di chirurgia plastica per la raccolta del tessuto adiposo sottocutaneo. P. Sukho è sostenuta da una sovvenzione dal programma The Netherlands Fellowship (NFP-12/435), durante lo svolgimento dello studio. Y.M. Bastiaansen-Jenniskens è supportato da sovvenzioni da olandese Arthritis Foundation (LP11).

Il tessuto adiposo sottocutaneo addominale è stato ottenuto da donatori umani con approvazione della Commissione etica medica (#MEC-2014-092), Erasmus MC University Medical Center, Rotterdam, Paesi Bassi. Tutti gli esperimenti sugli animali sono stati approvati dal comitato etico della sperimentazione animale, Erasmus MC University Medical Center, Rotterdam, Paesi Bassi (133-14-01).
1. umani ASCs isolamento e coltura
<ol><li>Preparare 3 mL di terreno di isolamento per 1 g di tessuto adiposo...

<ol>
	<li>Kawahara, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Single-incision+laparoscopic+right+colectomy+for+recurrent+Crohn's+disease.">Single-incision laparoscopic right colectomy for recurrent Crohn's disease.</a> Hepatogastroenterology. 57 (102-103), 1170-1172 (2010).</li><li>Saia, M., Buja, A., Mantoan, D., Agresta, F., Baldo, V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Colon+Cancer+Surgery:+A+Retrospective+Study+Based+on+a+Large+Administrative+Database.">Colon Cancer Surgery: A Retrospective Study Based on a Large Administrative Database.</a> Surg Laparo Endo Per. 26 (6), e126-e131 (2016).</li><li>Snijders, H. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Meta-analysis+of+the+risk+for+anastomotic+leakage,+the+postoperative+mortality+caused+by+leakage+in+relation+to+the+overall+postoperative+mortality.">Meta-analysis of the risk for anastomotic leakage, the postoperative mortality caused by leakage in relation to the overall postoperative mortality.</a> Eur J Surg Oncol. 38 (11), 1013-1019 (2012).</li><li>Daams, F., Luyer, M., Lange, J. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Colorectal+anastomotic+leakage:+aspects+of+prevention,+detection+and+treatment.">Colorectal anastomotic leakage: aspects of prevention, detection and treatment.</a> World J Gastroentero. 19 (15), 2293-2297 (2013).</li><li>Testini, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Bovine+pericardium+patch+wrapping+intestinal+anastomosis+improves+healing+process+and+prevents+leakage+in+a+pig+model.">Bovine pericardium patch wrapping intestinal anastomosis improves healing process and prevents leakage in a pig model.</a> PLoS One. 9 (1), e86627 (2014).</li><li>Kilroy, G. E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cytokine+profile+of+human+adipose-derived+stem+cells:+expression+of+angiogenic,+hematopoietic,+and+pro-inflammatory+factors.">Cytokine profile of human adipose-derived stem cells: expression of angiogenic, hematopoietic, and pro-inflammatory factors.</a> J Cell Physiol. 212 (3), 702-709 (2007).</li><li>Mussano, F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Growth+Factor+Profile+Characterization+of+Undifferentiated+and+Osteoinduced+Human+Adipose-Derived+Stem+Cells.">Growth Factor Profile Characterization of Undifferentiated and Osteoinduced Human Adipose-Derived Stem Cells.</a> Stem Cells Int. , 6202783 (2017).</li><li>Hassan, W. U., Greiser, U., Wang, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Role+of+adipose-derived+stem+cells+in+wound+healing.">Role of adipose-derived stem cells in wound healing.</a> Wound Repair Regen. 22 (3), 313-325 (2014).</li><li>Shudo, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Addition+of+mesenchymal+stem+cells+enhances+the+therapeutic+effects+of+skeletal+myoblast+cell-sheet+transplantation+in+a+rat+ischemic+cardiomyopathy+model.">Addition of mesenchymal stem cells enhances the therapeutic effects of skeletal myoblast cell-sheet transplantation in a rat ischemic cardiomyopathy model.</a> Tissue Eng Pt A. 20 (3-4), 728-739 (2014).</li><li>Otsuki, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Adipose+stem+cell+sheets+improved+cardiac+function+in+the+rat+myocardial+infarction,+but+did+not+alter+cardiac+contractile+responses+to+beta-adrenergic+stimulation.">Adipose stem cell sheets improved cardiac function in the rat myocardial infarction, but did not alter cardiac contractile responses to beta-adrenergic stimulation.</a> Biomed Res. 36 (1), 11-19 (2015).</li><li>Hamdi, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Epicardial+adipose+stem+cell+sheets+results+in+greater+post-infarction+survival+than+intramyocardial+injections.">Epicardial adipose stem cell sheets results in greater post-infarction survival than intramyocardial injections.</a> Cardiovasc Res. 91 (3), 483-491 (2011).</li><li>Cerqueira, M. T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Human+adipose+stem+cells+cell+sheet+constructs+impact+epidermal+morphogenesis+in+full-thickness+excisional+wounds.">Human adipose stem cells cell sheet constructs impact epidermal morphogenesis in full-thickness excisional wounds.</a> Biomacromolecules. 14 (11), 3997-4008 (2013).</li><li>Perrod, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cell+Sheet+Transplantation+for+Esophageal+Stricture+Prevention+after+Endoscopic+Submucosal+Dissection+in+a+Porcine+Model.">Cell Sheet Transplantation for Esophageal Stricture Prevention after Endoscopic Submucosal Dissection in a Porcine Model.</a> PLoS One. 11 (3), e0148249 (2016).</li><li>Pascual, I., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Adipose-derived+mesenchymal+stem+cells+in+biosutures+do+not+improve+healing+of+experimental+colonic+anastomoses.">Adipose-derived mesenchymal stem cells in biosutures do not improve healing of experimental colonic anastomoses.</a> Brit J Surg. 95 (9), 1180-1184 (2008).</li><li>Yoo, J. H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Adipose-tissue-derived+Stem+Cells+Enhance+the+Healing+of+Ischemic+Colonic+Anastomoses:+An+Experimental+Study+in+Rats.">Adipose-tissue-derived Stem Cells Enhance the Healing of Ischemic Colonic Anastomoses: An Experimental Study in Rats.</a> J Korean Soc Coloproctol. 28 (3), 132-139 (2012).</li><li>Okano, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cell+sheet+engineering.">Cell sheet engineering.</a> Rinsho Shinkeigaku. 46 (11), 795-798 (2006).</li><li>Chen, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Application+of+the+cell+sheet+technique+in+tissue+engineering.">Application of the cell sheet technique in tissue engineering.</a> Biomed Rep. 3 (6), 749-757 (2015).</li><li>Labbe, B., Marceau-Fortier, G., Fradette, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cell+sheet+technology+for+tissue+engineering:+the+self-assembly+approach+using+adipose-derived+stromal+cells.">Cell sheet technology for tissue engineering: the self-assembly approach using adipose-derived stromal cells.</a> Methods Mol Biol. 702, 429-441 (2011).</li><li>Sukho, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Adipose+Tissue-Derived+Stem+Cell+Sheet+Application+for+Tissue+Healing+In+Vivo:+A+Systematic+Review.">Adipose Tissue-Derived Stem Cell Sheet Application for Tissue Healing In Vivo: A Systematic Review.</a> Tissue Eng Part B-Re. , (2017).</li><li>Sukho, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+adipose+stem+cell+sheets+on+colon+anastomotic+leakage+in+an+experimental+model:+Proof+of+principle.">Effects of adipose stem cell sheets on colon anastomotic leakage in an experimental model: Proof of principle.</a> Biomaterials. 140, 69-78 (2017).</li><li>Verseijden, F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Angiogenic+capacity+of+human+adipose-derived+stromal+cells+during+adipogenic+differentiation:+an+in+vitro+study.">Angiogenic capacity of human adipose-derived stromal cells during adipogenic differentiation: an in vitro study.</a> Tissue Eng Pt A. 15 (2), 445-452 (2009).</li><li>Sukho, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+Cell+Seeding+Density+and+Inflammatory+Cytokines+on+Adipose+Tissue-Derived+Stem+Cells:+an+in+Vitro+Study.">Effect of Cell Seeding Density and Inflammatory Cytokines on Adipose Tissue-Derived Stem Cells: an in Vitro Study.</a> Stem Cell Rev. 13 (2), 267-277 (2017).</li><li>. Cell Harvesting by Temperature Reduction Available from: <a target="_blank" href="https://tools.thermofisher.com/content/sfs/brochures/AppNote-Nunc-UpCell-N20230.pdf">https://tools.thermofisher.com/content/sfs/brochures/AppNote-Nunc-UpCell-N20230.pdf</a> (2008)</li><li>Wu, Z., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Colorectal+anastomotic+leakage+caused+by+insufficient+suturing+after+partial+colectomy:+a+new+experimental+model.">Colorectal anastomotic leakage caused by insufficient suturing after partial colectomy: a new experimental model.</a> Surg Infect (Larchmt). 15 (6), 733-738 (2014).</li><li>Wu, Z., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reducing+colorectal+anastomotic+leakage+with+tissue+adhesive+in+experimental+inflammatory+bowel+disease.">Reducing colorectal anastomotic leakage with tissue adhesive in experimental inflammatory bowel disease.</a> Inflamm Bowel Dis. 21 (5), 1038-1046 (2015).</li><li>Wu, Z., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reducing+anastomotic+leakage+by+reinforcement+of+colorectal+anastomosis+with+cyanoacrylate+glue.">Reducing anastomotic leakage by reinforcement of colorectal anastomosis with cyanoacrylate glue.</a> Eur Surg Res. 50 (3-4), 255-261 (2013).</li><li>Aysan, E., Dincel, O., Bektas, H., Alkan, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Polypropylene+mesh+covered+colonic+anastomosis.+Results+of+a+new+anastomosis+technique.">Polypropylene mesh covered colonic anastomosis. Results of a new anastomosis technique.</a> Int J Surg. 6 (3), 224-229 (2008).</li><li>Suarez-Grau, J. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fibrinogen-thrombin+collagen+patch+reinforcement+of+high-risk+colonic+anastomoses+in+rats.">Fibrinogen-thrombin collagen patch reinforcement of high-risk colonic anastomoses in rats.</a> World J Gastrointest Surg. 8 (9), 627-633 (2016).</li><li>Kobayashi, J., Okano, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fabrication+of+a+thermoresponsive+cell+culture+dish:+a+key+technology+for+cell+sheet+tissue+engineering.">Fabrication of a thermoresponsive cell culture dish: a key technology for cell sheet tissue engineering.</a> Sci Technol Adv Mat. 11 (1), 014111 (2010).</li><li>Elloumi-Hannachi, I., Yamato, M., Okano, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cell+sheet+engineering:+a+unique+nanotechnology+for+scaffold-free+tissue+reconstruction+with+clinical+applications+in+regenerative+medicine.">Cell sheet engineering: a unique nanotechnology for scaffold-free tissue reconstruction with clinical applications in regenerative medicine.</a> J Intern Med. 267 (1), 54-70 (2010).</li><li>Miyahara, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Monolayered+mesenchymal+stem+cells+repair+scarred+myocardium+after+myocardial+infarction.">Monolayered mesenchymal stem cells repair scarred myocardium after myocardial infarction.</a> Nat Med. 12 (4), 459-465 (2006).</li><li>Makarevich, P. I., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Enhanced+angiogenesis+in+ischemic+skeletal+muscle+after+transplantation+of+cell+sheets+from+baculovirus-transduced+adipose-derived+stromal+cells+expressing+VEGF165.">Enhanced angiogenesis in ischemic skeletal muscle after transplantation of cell sheets from baculovirus-transduced adipose-derived stromal cells expressing VEGF165.</a> Stem Cell Res Ther. 6, 204 (2015).</li></ol>

Anastomotiche è l'evento avverso più grave a seguito di resezione del colon con un'anastomosi primaria. Tecniche ottimali per prevenire perdita e rottura anastomotic sono ancora carenti. Applicazione locale di una matrice di biomateriali è stata condotta, con diversi risultati25,26,27. L'obiettivo delle terapie di cellula è quello di facilitare la riparazione dei tessuti da sostituzione di tessuto o la stimolazione della gua...

Colectomia parziale con un'anastomosi primaria è un intervento chirurgico comunemente effettuato che può essere fatto per molte malattie che colpiscono il colon come cancro colorettale, morbo di Crohn e diverticolite1,2. Il più temuto complicazione dopo l'anastomosi colorettale è anastomotiche3. Sebbene siano stati identificati diversi fattori di rischio connessi con perdite anastomotiche, soluzioni per prevenire perdite rimangono unkown4,5.
Delle cellule stromali derivate da tessuto adiposo (ASCs) sono associate con le proprietà antinfiammatorie e trofiche6,7, che rende queste cellule promettenti candidati per terapie rigenerative8. L'efficacia di ASCs per promuovere la guarigione dei tessuti è stata dimostrata in vari tessuti quali il muscolo cardiaco, pelle ed esofago9,10,11,12,13. Tuttavia, ci sono pochi rapporti sull'uso di ASCs per promuovere la guarigione intestinale. Trapianto locale di ASCs per anastomosi colorettale sperimentale via rivestite con ASC biosutures o iniezioni serosal dell'ASC ha mostrato entrambi nessun miglioramento nella guarigione14 o non impedì anastomotiche nonostante una più favorevole anastomotica 15la guarigione.
Il trapianto locale di ASC in sospensione o in combinazione con biomateriali potrebbero essere associati con la conservazione delle cellule insufficiente o un'inadeguata distribuzione delle cellule trapiantate11. Cella foglio tecnologia16,17 offre un innovativo metodo di consegna ASC18,19. Pertanto, in uno studio precedente, un nuovo approccio è stato proposto in cui ASCs organizzato in una cella foglio potrebbe essere applicata su anastomosi colorettale sperimentale20. Questo studio ha dimostrato che il trapianto di foglio ASC è riuscito a ridurre le perdite di anastomosi colorettale dopo colectomia parziale in un modello del ratto. Questo articolo segnala ASC foglio preparazione e tecnica chirurgica di trapianto.

<table border="0" cellpadding="0" cellspacing="0" width="689">
	<tbody>
		<tr height="43">
			<td height="43" style="height:43px;width:216px;">LG DMEM</td>
			<td style="width:115px;">Gibco, Life technologies</td>
			<td style="width:191px;">22320022</td>
			<td style="width:167px;">ASC isolation and culture</td>
		</tr>
		<tr height="43">
			<td height="43" style="height:43px;width:216px;">Collagenase type I</td>
			<td style="width:115px;">Gibco, Life technologies</td>
			<td style="width:191px;">17100-01</td>
			<td style="width:167px;">ASC Isolation</td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:216px;">Bovine Serum Albumin</td>
			<td style="width:115px;">Sigma-Aldrich</td>
			<td style="width:191px;">A9418</td>
			<td style="width:167px;">ASC Isolation</td>
		</tr>
		<tr height="43">
			<td height="43" style="height:43px;width:216px;">Fetal bovine serum</td>
			<td style="width:115px;">Gibco, Life technologies</td>
			<td style="width:191px;">10270-106</td>
			<td style="width:167px;">FBS, ASC isolation and culture</td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:216px;">3% acetic acid with methylene blue</td>
			<td style="width:115px;">Stemcell Technologies</td>
			<td style="width:191px;">7060</td>
			<td style="width:167px;">ASC Isolation</td>
		</tr>
		<tr height="43">
			<td height="43" style="height:43px;width:216px;">Gentamicin</td>
			<td style="width:115px;">Gibco, Life technologies</td>
			<td style="width:191px;">15750-037</td>
			<td style="width:167px;">ASC isolation and culture</td>
		</tr>
		<tr height="43">
			<td height="43" style="height:43px;width:216px;">Ampothericin B&#160;</td>
			<td style="width:115px;">Gibco, Life technologies</td>
			<td style="width:191px;">15290-018</td>
			<td style="width:167px;">ASC isolation and culture</td>
		</tr>
		<tr height="64">
			<td height="64" style="height:64px;width:216px;">Shaker (Gallenkamp Environmental Shaker Model 10X 400)</td>
			<td style="width:115px;">Akribis Scientific</td>
			<td style="width:191px;">F240</td>
			<td style="width:167px;">ASC Isolation</td>
		</tr>
		<tr height="43">
			<td height="43" style="height:43px;width:216px;">Centrifuge&#160;</td>
			<td style="width:115px;">Hettichlab&#160;</td>
			<td style="width:191px;">Rotina380</td>
			<td style="width:167px;">ASC isolation and culture</td>
		</tr>
		<tr height="62">
			<td height="62" style="height:62px;width:216px;">Phosphate buffer saline&#160;</td>
			<td style="width:115px;">Gibco, Life technologies</td>
			<td style="width:191px;">14190-094</td>
			<td style="width:167px;">PBS, ASC isolation and culture</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Ascorbic acid-2-phosphate</td>
			<td style="width:115px;">Sigma-Aldrich</td>
			<td style="width:191px;">A8960</td>
			<td style="width:167px;">ASC isolation and culture</td>
		</tr>
		<tr height="37">
			<td height="37" style="height:37px;width:216px;">Human recombinant fibroblast growth factor 2&#160;</td>
			<td style="width:115px;">AbD Serotec</td>
			<td style="width:191px;">AF-100-15</td>
			<td style="width:167px;">FGF2, ASC isolation and culture</td>
		</tr>
		<tr height="43">
			<td height="43" style="height:43px;width:216px;">Trypsin EDTA</td>
			<td style="width:115px;">Gibco, Life technologies</td>
			<td style="width:191px;">25200-056</td>
			<td style="width:167px;">ASC culture</td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:216px;">Dimethyl sulfoxide</td>
			<td style="width:115px;">Sigma-Aldrich</td>
			<td style="width:191px;">D2650-5x</td>
			<td style="width:167px;">DMSO, ASC freezing</td>
		</tr>
		<tr height="43">
			<td height="43" style="height:43px;width:216px;">Thermo-responsive culture dishes</td>
			<td style="width:115px;">Nunc Thermo scientific</td>
			<td style="width:191px;">174904</td>
			<td style="width:167px;">ASC sheet preperation</td>
		</tr>
		<tr height="43">
			<td height="43" style="height:43px;width:216px;">Non-absorbable braided silk 4/0</td>
			<td style="width:115px;">B Braun</td>
			<td style="width:191px;">21151042</td>
			<td style="width:167px;">surgery</td>
		</tr>
		<tr height="43">
			<td height="43" style="height:43px;width:216px;">Non-absorbable monofilament polyamide&#160; 8/0</td>
			<td style="width:115px;">B Braun</td>
			<td style="width:191px;">G1118170</td>
			<td style="width:167px;">surgery</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Absorbable braided polyglycolic acid 5/0</td>
			<td style="width:115px;">B Braun</td>
			<td style="width:191px;">C1048207</td>
			<td style="width:167px;">surgery</td>
		</tr>
	</tbody>
</table>

transplantation of adipose tissue-derived stem cell sheet to reduce leakage after partial colectomy in a rat model

Anastomotiche è una complicazione disastrosa dopo la chirurgia del colon-retto. Sebbene metodi correnti per la prevenzione di perdite hanno diversi livelli di efficacia clinica, sono fino a questo momento imperfette soluzioni. Terapia con cellule staminali utilizzando fogli di ASC potrebbe fornire una soluzione a questo problema. ASC sono considerati come promettenti candidati per la promozione del tessuto di guarigione a causa delle loro proprietà trofiche e immunomodulatori. Qui, mettiamo a disposizione metodi per produrre fogli ASC ad alta densità, che vengono trapiantate su un'anastomosi colorettale in un modello del ratto per ridurre la perdita. ASC formano strati delle cellule in piastre di coltura termo-sensibile che potrebbero essere facilmente smontati. Il giorno del trapianto, è stata eseguita una colectomia parziale con un'anastomosi colorettale 5-sutura. Gli animali sono stati trapiantati immediatamente con 1 foglio ASC per ratto. Fogli di ASC aderito spontaneamente all'anastomosi senza alcun collante, sutura o qualsiasi biomateriale. Gruppi di animali sono stati sacrificati 3 e 7 giorni postoperatorio. Confrontato agli animali trapiantati, l'incidenza degli ascessi anastomotic e perdita era maggiore negli animali di controllo. Nel nostro modello, il trapianto di fogli ASC dopo l'anastomosi colorettale è stata associata con un tasso di perdite inferiore e di successo.

Un diagramma di flusso di questo studio raffigurante sia cultura foglio ASC e la procedura di resezione del colon e l'anastomosi è illustrato nella Figura 1. La figura 2 Mostra la morfologia microscopica foglio ASC e l'apparenza macroscopica del foglio ASC durante e dopo la separazione. La figura 3 Mostra le diverse fasi di distacco del foglio ASC e trapianto. La figura 4</stron...

Watch this Scientific Journal Video about Transplantation of Adipose Tissue-Derived Stem Cell Sheet to Reduce Leakage After Partial Colectomy in A Rat Model at JoVE.com

Transplantation of Adipose Tissue-Derived Stem Cell Sheet to Reduce Leakage After Partial Colectomy in A Rat Model

Trapianto di cellule staminali di tessuto adiposo-derivato foglio per ridurre le perdite dopo la colectomia parziale in un modello del ratto

La preparazione e il trapianto di tessuto adiposo derivato della cellula formativa (ASC) fogli sul insufficientemente suturate anastomosi colorettale in un modello del ratto è presentata. Questa applicazione romanzo mostra che fogli di ASC possono ridurre le perdite di anastomosi colorettale.

Anastomotic leakage is a disastrous complication after colorectal surgery. Although current methods for leakage prevention have different levels of ...

Research

JoVE Journal

Medicine

Orthotopic Aortic Transplantation: A Rat Model to Study the Development of Chronic Vasculopathy

Trapianto ortotopico aortica: un modello di ratto per studiare lo sviluppo di vasculopatia cronica

Research models of chronic rejection are essential to investigate pathobiological and pathophysiological processes during the development of transplant ...

Ricerca

Medicina

A Mouse Model of in Utero Transplantation

A Mouse Model of in Utero Transplantation

Un modello murino di Nell&#39;utero Trapianto

The transplantation of stem cells and viruses in utero has tremendous potential for treating congenital disorders in the human fetus.  For example, in ...

Stem Cell Transplantation in an in vitro Simulated Ischemia/Reperfusion Model

Stem Cell Transplantation in an in vitro Simulated Ischemia/Reperfusion Model

Trapianto di cellule staminali in un In vitro Simulato ischemia / riperfusione Modello

Stem cell transplantation protocols are finding their way into clinical practice1,2,3. Getting better results, making the protocols more robust, and ...

Surgical Procedures for a Rat Model of Partial Orthotopic Liver Transplantation with Hepatic Arterial Reconstruction

Procedure chirurgiche per un modello di Rat parziale trapianto di fegato con la ricostruzione arteriosa epatica

Orthotopic liver transplantation (OLT) in rats using a whole or partial graft is an indispensable experimental model for transplantation research, such as ...

Neural Stem Cell Transplantation in Experimental Contusive Model of Spinal Cord Injury

Neural Stem Cell Transplantation in Sperimentale Contusivi Model of Spinal Cord Injury

Spinal cord injury is a devastating clinical condition, characterized by a complex of neurological dysfunctions. Animal models of spinal cord injury can ...

Creation and Transplantation of an Adipose-derived Stem Cell (ASC) Sheet in a Diabetic Wound-healing Model

Creation and Transplantation of an Adipose-derived Stem Cell ASC Sheet in a Diabetic Wound-healing Model

Creazione e il trapianto di un foglio di cellule staminali di derivazione adiposa (ASC) in un modello diabetico di guarigione

Artificial skin has achieved considerable therapeutic results in clinical practice. However, artificial skin treatments for wounds in diabetic patients ...

ADSC-sheet Transplantation to Prevent Stricture after Extended Esophageal Endoscopic Submucosal Dissection

ADSC fogli trapianto per prevenire stenosi dopo esteso esofagea endoscopica della sottomucosa dissezione

In past years, the cell-sheet construct has spurred wide interest in regenerative medicine, especially for reconstructive surgery procedures. The ...

Intrasplenic Transplantation of Hepatocytes After Partial Hepatectomy in NOD.SCID Mice

Intrasplenic trapianto di epatociti dopo epatectomia parziale in NOD. Topi SCID

Partial hepatectomy is a versatile and reproducible method to study liver regeneration and the effect of cell based therapeutics in various pathological ...

Re-Arterialized Rat Partial Liver Transplantation with an in vivo Vessel-Oriented 70% Hepatectomy

Re-Arterialized Rat Partial Liver Transplantation with an in vivo Vessel-Oriented 70% Hepatectomy

Ri-Arterialized trapianto parziale di fegato del ratto con un in vivo vaso-Oriented 70% Hepatectomy

Split liver transplantation and living liver donor liver transplantation were developed in the clinic to utilize liver organs in a more efficient manner. ...

Reduced Complications after Arterial Reconnection in a Rat Model of Orthotopic Liver Transplantation

Complicazioni ridotte dopo la riconnessione arteriosa in un modello di ratto di trapianto di fegato ortotopico

The rat orthotopic liver transplantation (OLT) model is a powerful tool to study acute and chronic rejection. However, it is not a complete representation ...