Name: transplantation of adipose tissue-derived stem cell sheet to reduce leakage after partial colectomy in a rat model
Uploaded: 2018-08-11T12:30:00
Description: Watch this Scientific Journal Video about Transplantation of Adipose Tissue-Derived Stem Cell Sheet to Reduce Leakage After Partial Colectomy in A Rat Model at JoVE.com

저자는 교수 박사 S.E.R. Hovius, 박사 M.A.M. Mureau 및 모든 외과 피하 지방이 많은 직물의 컬렉션에 대 한 성형 수술의 부의 감사. P. Sukho 연구의 행위 동안에 네덜란드 친목 프로그램 (NFP-12/435)에서 교부 금에 의해 지원 됩니다. 사원 Bastiaansen Jenniskens 네덜란드 관절염 재단 (LP11)에서 교부 금에 의해 지원 됩니다.

복 부 피하 지방 조직 승인 의료 윤리 위원회 (#MEC-2014-092), 에라스무스 엠씨 대학 의료 센터, 로테르 담, 네덜란드의 인간 기증자 로부터 얻은 것입니다. 모든 동물 실험 윤리 위원회의 동물 실험, 에라스무스 엠씨 대학 의료 센터, 로테르 담, 네덜란드 (133-14-01)에 의해 승인 되었다.
1. 인간의 ASCs 격리와 문화
<ol><li>1g의 지방 조직에 대 한 절연 매체의 3 mL를 준비 합니다. 혼합 ?...

<ol>
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Cell Harvesting by Temperature Reduction Available from: <a target="_blank" href="https://tools.thermofisher.com/content/sfs/brochures/AppNote-Nunc-UpCell-N20230.pdf">https://tools.thermofisher.com/content/sfs/brochures/AppNote-Nunc-UpCell-N20230.pdf</a> (2008)</li><li>Wu, Z., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Colorectal+anastomotic+leakage+caused+by+insufficient+suturing+after+partial+colectomy:+a+new+experimental+model.">Colorectal anastomotic leakage caused by insufficient suturing after partial colectomy: a new experimental model.</a> Surg Infect (Larchmt). 15 (6), 733-738 (2014).</li><li>Wu, Z., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reducing+colorectal+anastomotic+leakage+with+tissue+adhesive+in+experimental+inflammatory+bowel+disease.">Reducing colorectal anastomotic leakage with tissue adhesive in experimental inflammatory bowel disease.</a> Inflamm Bowel Dis. 21 (5), 1038-1046 (2015).</li><li>Wu, Z., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reducing+anastomotic+leakage+by+reinforcement+of+colorectal+anastomosis+with+cyanoacrylate+glue.">Reducing anastomotic leakage by reinforcement of colorectal anastomosis with cyanoacrylate glue.</a> Eur Surg Res. 50 (3-4), 255-261 (2013).</li><li>Aysan, E., Dincel, O., Bektas, H., Alkan, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Polypropylene+mesh+covered+colonic+anastomosis.+Results+of+a+new+anastomosis+technique.">Polypropylene mesh covered colonic anastomosis. 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Anastomotic 누설은 기본 문 합으로 결 장 절제술을 다음과 같은 가장 심각한 불리 한 이벤트 이다. Anastomotic 장애 및 누설을 방지 하기 위해 최적의 기술을 여전히 부족 한. 다양 한 생체 재료의 로컬 응용 프로그램, 다양 한 결과25,,2627실시 되어 있다. 세포 치료의 목표는 조직 대체 하 여 조직 복구 또는 로컬 치유 통해 paracrine 분 ?...

기본 문 합으로 부분 colectomy는 대 장 암, 크 론 병, 계실 염1,2등 콜론에 영향을 미치는 많은 질병에 대 한 할 수 있는 일반적으로 수행된 수술입니다. 가장 후 대 장 문 합은 anastomotic 누설3합병증을 겁 나. 비록 anastomotic 누수와 관련 된 몇 가지 위험 요소 확인 되었습니다, 누설을 방지 하기 위한 솔루션 알4,5남아 있습니다.
지방 조직 유래 stromal 세포 (ASCs) 염증 및 영양 특성6,7, 이러한 세포 재생 요법8에 대 한 후보를 유망 하 게 연결 되어 있습니다. 조직 치유를 촉진 하는 ASCs의 효과 심장 근육, 피부, 식도9,,1011,,1213등 다양 한 조직에 표시 했다. 그러나, 장 치유를 촉진 하는 ASCs 사용 하 여에 몇 가지 보고 있다. ASC 코팅 biosutures 통해 실험 대 장 anastomoses에 ASCs의 지방 이식 또는 ASCs serosal 주사14 치유 하거나 개선 보였다 anastomotic 더 유리한도 불구 하 고 anastomotic 누설을 방지 하지 않았다 치유15.
정지에서 ASCs의 지방 이식 함께 또는 생체 부족 세포 보존 또는 이식된 세포11의 부적 절 한 배포에 연관 될 수도 있습니다. 셀 시트 기술16,17 ASC 배달18,19의 혁신적인 방법을 제공합니다. 따라서, 이전 연구에 새로운 접근 방식은 어느 ASCs 셀에 시트 실험 대 장 문 합20에 적용 수 있는 제안 했다. 이 연구는 ASC 시트 이식 쥐 모델에서 부분 colectomy 후 대 장 문 합 누출을 감소 시키기에서 성공적 증명. 이 기사 보고 ASC 시트 준비 및 외과 이식 기술 합니다.

<table border="0" cellpadding="0" cellspacing="0" width="689">
	<tbody>
		<tr height="43">
			<td height="43" style="height:43px;width:216px;">LG DMEM</td>
			<td style="width:115px;">Gibco, Life technologies</td>
			<td style="width:191px;">22320022</td>
			<td style="width:167px;">ASC isolation and culture</td>
		</tr>
		<tr height="43">
			<td height="43" style="height:43px;width:216px;">Collagenase type I</td>
			<td style="width:115px;">Gibco, Life technologies</td>
			<td style="width:191px;">17100-01</td>
			<td style="width:167px;">ASC Isolation</td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:216px;">Bovine Serum Albumin</td>
			<td style="width:115px;">Sigma-Aldrich</td>
			<td style="width:191px;">A9418</td>
			<td style="width:167px;">ASC Isolation</td>
		</tr>
		<tr height="43">
			<td height="43" style="height:43px;width:216px;">Fetal bovine serum</td>
			<td style="width:115px;">Gibco, Life technologies</td>
			<td style="width:191px;">10270-106</td>
			<td style="width:167px;">FBS, ASC isolation and culture</td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:216px;">3% acetic acid with methylene blue</td>
			<td style="width:115px;">Stemcell Technologies</td>
			<td style="width:191px;">7060</td>
			<td style="width:167px;">ASC Isolation</td>
		</tr>
		<tr height="43">
			<td height="43" style="height:43px;width:216px;">Gentamicin</td>
			<td style="width:115px;">Gibco, Life technologies</td>
			<td style="width:191px;">15750-037</td>
			<td style="width:167px;">ASC isolation and culture</td>
		</tr>
		<tr height="43">
			<td height="43" style="height:43px;width:216px;">Ampothericin B&#160;</td>
			<td style="width:115px;">Gibco, Life technologies</td>
			<td style="width:191px;">15290-018</td>
			<td style="width:167px;">ASC isolation and culture</td>
		</tr>
		<tr height="64">
			<td height="64" style="height:64px;width:216px;">Shaker (Gallenkamp Environmental Shaker Model 10X 400)</td>
			<td style="width:115px;">Akribis Scientific</td>
			<td style="width:191px;">F240</td>
			<td style="width:167px;">ASC Isolation</td>
		</tr>
		<tr height="43">
			<td height="43" style="height:43px;width:216px;">Centrifuge&#160;</td>
			<td style="width:115px;">Hettichlab&#160;</td>
			<td style="width:191px;">Rotina380</td>
			<td style="width:167px;">ASC isolation and culture</td>
		</tr>
		<tr height="62">
			<td height="62" style="height:62px;width:216px;">Phosphate buffer saline&#160;</td>
			<td style="width:115px;">Gibco, Life technologies</td>
			<td style="width:191px;">14190-094</td>
			<td style="width:167px;">PBS, ASC isolation and culture</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Ascorbic acid-2-phosphate</td>
			<td style="width:115px;">Sigma-Aldrich</td>
			<td style="width:191px;">A8960</td>
			<td style="width:167px;">ASC isolation and culture</td>
		</tr>
		<tr height="37">
			<td height="37" style="height:37px;width:216px;">Human recombinant fibroblast growth factor 2&#160;</td>
			<td style="width:115px;">AbD Serotec</td>
			<td style="width:191px;">AF-100-15</td>
			<td style="width:167px;">FGF2, ASC isolation and culture</td>
		</tr>
		<tr height="43">
			<td height="43" style="height:43px;width:216px;">Trypsin EDTA</td>
			<td style="width:115px;">Gibco, Life technologies</td>
			<td style="width:191px;">25200-056</td>
			<td style="width:167px;">ASC culture</td>
		</tr>
		<tr height="22">
			<td height="22" style="height:22px;width:216px;">Dimethyl sulfoxide</td>
			<td style="width:115px;">Sigma-Aldrich</td>
			<td style="width:191px;">D2650-5x</td>
			<td style="width:167px;">DMSO, ASC freezing</td>
		</tr>
		<tr height="43">
			<td height="43" style="height:43px;width:216px;">Thermo-responsive culture dishes</td>
			<td style="width:115px;">Nunc Thermo scientific</td>
			<td style="width:191px;">174904</td>
			<td style="width:167px;">ASC sheet preperation</td>
		</tr>
		<tr height="43">
			<td height="43" style="height:43px;width:216px;">Non-absorbable braided silk 4/0</td>
			<td style="width:115px;">B Braun</td>
			<td style="width:191px;">21151042</td>
			<td style="width:167px;">surgery</td>
		</tr>
		<tr height="43">
			<td height="43" style="height:43px;width:216px;">Non-absorbable monofilament polyamide&#160; 8/0</td>
			<td style="width:115px;">B Braun</td>
			<td style="width:191px;">G1118170</td>
			<td style="width:167px;">surgery</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Absorbable braided polyglycolic acid 5/0</td>
			<td style="width:115px;">B Braun</td>
			<td style="width:191px;">C1048207</td>
			<td style="width:167px;">surgery</td>
		</tr>
	</tbody>
</table>

transplantation of adipose tissue-derived stem cell sheet to reduce leakage after partial colectomy in a rat model

Anastomotic 누설 대 장 수술 후 비참 한 합병증은 이다. 누출 방지에 대 한 현재 메서드는 임상 효능의 다른 수준, 그들은 지금 불완전 한 솔루션까지 있습니다. 줄기 세포 치료 ASC 시트를 사용 하 여이 문제에 대 한 솔루션을 제공할 수 있습니다. ASCs 조직 그들의 영양 및 immunomodulatory 속성 때문에 치유를 승진 시키기를 위한 유망 후보자 이라고 여겨진다. 여기, 우리는 누설을 줄이기 위해 쥐 모델에서 대 장 문 합에 이식 하는 고밀도 ASC 시트를 생산 하는 방법을 제공 합니다. ASCs 셀 시트 쉽게 분리 될 수 있는 온도 응답 문화 요리에 형성. 이식의 날, 5 봉합 대 장 문 합으로 부분 colectomy 수행 되었다. 동물 쥐 당 1 ASC 시트와 함께 즉시 이식 했다. ASC 시트 없이 어떤 접착제, 봉합 사, 또는 어떤 소재 문 합을 자발적으로 준수 됩니다. 동물 그룹 3 ~ 7 일을 희생 했다 postoperatively. 이식된 동물에 비해, anastomotic 농 양 및 누설의 부각 더 높았다 제어 동물. 우리의 모델에서 대 장 문 합 후 ASC 시트 이식 성공적이 고 낮은 누설 율으로 연결 했다.

ASC 시트 문화 및 결 장 절제술 및 문 합 절차를 묘사 하는이 연구의 흐름 차트는 그림 1에 표시 됩니다. 그림 2 는 ASC 시트 현미경 형태학 및 중 고 분리 후 ASC 시트의 거시적인 외관. 그림 3 ASC 시트 분리 및 이식의 다른 단계를 보여 줍니다. 그림 4 는 anastomotic 선 및 누설 방지에 ASC 시?...

Watch this Scientific Journal Video about Transplantation of Adipose Tissue-Derived Stem Cell Sheet to Reduce Leakage After Partial Colectomy in A Rat Model at JoVE.com

Transplantation of Adipose Tissue-Derived Stem Cell Sheet to Reduce Leakage After Partial Colectomy in A Rat Model

쥐 모델에 부분 Colectomy 후 누설을 줄이기 위해 지방 조직 유래 줄기 세포의 이식

준비와 쥐 모델에서 부족 하 게 봉합 대 장 anastomoses에 지방 조직 유래 줄기 세포 (ASC) 시트의 이식 제공 됩니다. 이 새로운 응용 프로그램은 ASC 시트 대 장 문 합 누출을 줄일 수 있습니다 보여줍니다.

Anastomotic leakage is a disastrous complication after colorectal surgery. Although current methods for leakage prevention have different levels of ...

Research

JoVE Journal

Medicine

Orthotopic Aortic Transplantation: A Rat Model to Study the Development of Chronic Vasculopathy

Orthotopic 대동맥 이식 : 만성 Vasculopathy의 개발 연구에 생쥐 모델

Research models of chronic rejection are essential to investigate pathobiological and pathophysiological processes during the development of transplant ...

연구

의학

A Mouse Model of in Utero Transplantation

A Mouse Model of in Utero Transplantation

의 마우스 모델 Utero에 이식

The transplantation of stem cells and viruses in utero has tremendous potential for treating congenital disorders in the human fetus.  For example, in ...

Stem Cell Transplantation in an in vitro Simulated Ischemia/Reperfusion Model

Stem Cell Transplantation in an in vitro Simulated Ischemia/Reperfusion Model

에서 간세포 이식 체외에서 시뮬레이션 국소 빈혈 / Reperfusion 모델

Stem cell transplantation protocols are finding their way into clinical practice1,2,3. Getting better results, making the protocols more robust, and ...

Surgical Procedures for a Rat Model of Partial Orthotopic Liver Transplantation with Hepatic Arterial Reconstruction

간 간선 재건과 부분 Orthotopic 간 이식의 쥐 모델에 대한 외과 절차

Orthotopic liver transplantation (OLT) in rats using a whole or partial graft is an indispensable experimental model for transplantation research, such as ...

Neural Stem Cell Transplantation in Experimental Contusive Model of Spinal Cord Injury

척수 손상의 실험 Contusive 모델에서 신경 줄기 세포 이식

Spinal cord injury is a devastating clinical condition, characterized by a complex of neurological dysfunctions. Animal models of spinal cord injury can ...

Creation and Transplantation of an Adipose-derived Stem Cell (ASC) Sheet in a Diabetic Wound-healing Model

Creation and Transplantation of an Adipose-derived Stem Cell ASC Sheet in a Diabetic Wound-healing Model

생성 및 당뇨병 치유 하는 부상을 모델에서 줄기 세포 지방이 많은 파생 (ASC) 시트의 이식

Artificial skin has achieved considerable therapeutic results in clinical practice. However, artificial skin treatments for wounds in diabetic patients ...

ADSC-sheet Transplantation to Prevent Stricture after Extended Esophageal Endoscopic Submucosal Dissection

ADSC 매 이식은 확장 식도 내시경 점막하 절개 후 협착을 방지하는

In past years, the cell-sheet construct has spurred wide interest in regenerative medicine, especially for reconstructive surgery procedures. The ...

Intrasplenic Transplantation of Hepatocytes After Partial Hepatectomy in NOD.SCID Mice

끄 덕 임에서 부분 Hepatectomy 후 Hepatocytes의 intrasplenic 이식 SCID 마우스

Partial hepatectomy is a versatile and reproducible method to study liver regeneration and the effect of cell based therapeutics in various pathological ...

Re-Arterialized Rat Partial Liver Transplantation with an in vivo Vessel-Oriented 70% Hepatectomy

Re-Arterialized Rat Partial Liver Transplantation with an in vivo Vessel-Oriented 70% Hepatectomy

다시는 vivo에서 선박 지향 70% 쥐 부분 간 이식 Arterialized Hepatectomy

Split liver transplantation and living liver donor liver transplantation were developed in the clinic to utilize liver organs in a more efficient manner. ...

Reduced Complications after Arterial Reconnection in a Rat Model of Orthotopic Liver Transplantation

직교 간 이식의 쥐 모형에 있는 동맥 재연결 후에 감소된 합병증

The rat orthotopic liver transplantation (OLT) model is a powerful tool to study acute and chronic rejection. However, it is not a complete representation ...