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DOI :
10.3791/57278-v
•
8:27 min
April 10th, 2018
Chapters
0:05
Title
1:05
sgRNA Fwd Primer Design, DNA Template Synthesis, and ln Vitro Transcription of sgRNA
3:38
Cas9-sgRNA Complexing and Electroporation
5:45
Results: Use of Cas9-sgRNA Electroporation Protocol to Study Efficient Knockout of Target Gene
7:48
Conclusion
本文介绍了一种快速 CRISPR/Cas9-mediated 基因在小鼠和人原发性造血细胞中的破坏的协议。Cas9-sgRNA ribonucleoproteins 是通过电穿孔与 sgRNAs 产生通过体外转录和商业 Cas9。在有限的时间和财务成本下实现了高编辑效率。
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