Name: collecting hair samples for hair cortisol analysis in african americans
Uploaded: 2018-06-10T14:00:00
Description: Watch this Scientific Journal Video about Collecting Hair Samples for Hair Cortisol Analysis in African Americans at JoVE.com

这出版物由克利夫兰的临床和翻译科学合作使成为可能;KL2TR000440 国家卫生研究院 (NCATS) 国家促进转化科学中心;和 NIH 的医学研究路线图。朱迪. 福特博士在这一领域的工作由国立国家药物滥用研究所 (1R21DA034960) 和俄亥俄州立大学人口研究研究所资助, 由国立儿童健康研究所和发展 (2P2CHD058484)。内容完全是作者的责任, 不一定代表国家卫生研究院的官方意见。Laudenslager 博士在这一领域的工作是由以病人为中心的结果研究所 (CE-1304-6208) 的一份合同提供资金的, 这是由儿童和家庭当局 (90YR0058) 提供的补助金, 以及发展 Psychobiology 的补助金。科罗拉多大学丹佛安舒茨医学院精神病学系研究组捐赠基金。

这项研究获得了克利夫兰医疗中心机构审查委员会的批准, 该议定书遵循了审查委员会所指出的参加者保护和数据安全准则。
1. 为头发样本和发样收集问题准备参与者
<ol><li>向参加者解释, 收集的毛发样本比从头部后部 (后顶点) 每天刷牙的头发少。告诉参与者, 头发样本的网站是由周围的头发隐藏, 因此, 收集后不可见。通知参与者, 样品将被用来测量一种叫做皮质醇的荷尔?...

<ol>
	<li>Sauve, B. K. G., Walsh, G., Tokmakejian, S., Van Uum, S. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Measurement+of+cortisol+in+human+hair+as+a+biomarker+of+systemic+exposure.">Measurement of cortisol in human hair as a biomarker of systemic exposure.</a> Clin Invest Med. 30 (5), 183-191 (2007).</li><li>Wright, K. D., Hickman, R., Laudenslager, M. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hair+cortisol+analysis:+a+promising+biomarker+of+HPA+activation+in+older+adults.">Hair cortisol analysis: a promising biomarker of HPA activation in older adults.</a> Gerontologist. 55, 140-145 (2015).</li><li>Awad, G. H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Beauty+and+body+image+concerns+among+African+American+college+women.">Beauty and body image concerns among African American college women.</a> J Black Psychol. 41 (6), 540-564 (2015).</li><li>Capodilupo, C. M., Kim, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Gender+and+race+matter:+the+importance+of+considering+intersections+in+Black+women's+body+image.">Gender and race matter: the importance of considering intersections in Black women's body image.</a> J Couns Psychol. 61 (1), 37-49 (2014).</li><li>Miracle, V. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Belmont+Report:+the+triple+crown+of+research+ethics.">The Belmont Report: the triple crown of research ethics.</a> Dimens Crit Care Nurs. 35 (4), 223-228 (2016).</li><li>Fishman, B. M., Bobo, L., Kosub, K., Womeodu, R. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cultural+issues+in+serving+minority+populations:+emphasis+on+Mexican+Americans+and+African+Americans.">Cultural issues in serving minority populations: emphasis on Mexican Americans and African Americans.</a> Am J Med Sci. 306 (3), 160-166 (1993).</li><li>Webb, J. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Louisiana+voodoo+and+superstitions+related+to+health.">Louisiana voodoo and superstitions related to health.</a> HSMHA Health Rep. 86 (4), 291-301 (1971).</li><li>Ford, J. L., Boch, S. J., McCarthy, D. O. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Feasibility+of+hair+collection+for+cortisol+measurement+in+population+research+on+adolescent+health.">Feasibility of hair collection for cortisol measurement in population research on adolescent health.</a> Nurs Res. 65 (3), 249-255 (2016).</li><li>Wosu, A. C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hair+cortisol+in+relation+to+sociodemographic+and+lifestyle+characteristics+in+a+multiethnic+US+sample.">Hair cortisol in relation to sociodemographic and lifestyle characteristics in a multiethnic US sample.</a> Ann Epidemiol. 25 (2), 90-95 (2015).</li><li>Webb, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Assessing+individual+systemic+stress+through+cortisol+analysis+of+archaeological+hair.">Assessing individual systemic stress through cortisol analysis of archaeological hair.</a> J Archaeolog Sci. 37 (4), 807-812 (2010).</li><li>Russell, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Toward+standardization+of+hair+cortisol+measurement:+results+of+the+first+international+interlaboratory+round+robin.">Toward standardization of hair cortisol measurement: results of the first international interlaboratory round robin.</a> Ther Drug Monit. 37 (1), 71-75 (2015).</li><li>Bosley, R. E., Daveluy, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+primer+to+natural+hair+care+practices+in+black+patients.">A primer to natural hair care practices in black patients.</a> Cutis. 95 (2), 78-80 (2015).</li><li>Loussouarn, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=African+hair+growth+parameters.">African hair growth parameters.</a> Brit J Derm. 145 (2), 294-297 (2001).</li><li>Hoffman, M. C., Karban, L. V., Goodteacher, A., Laudenslager, M. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chemical+processing+and+shampooing+impact+cortisol+measured+in+human+hair.">Chemical processing and shampooing impact cortisol measured in human hair.</a> Clin Invest Med. 37 (4), 252-257 (2014).</li><li>Ramirez, J., Elmofty, M., Castillo, E., DeRouen, M., Shariff-Marco, S., Allen, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Evaluation+of+cortisol+and+telomere+length+measurements+in+ethnically+diverse+women+with+breast+cancer+using+culturally+sensitive+methods.">Evaluation of cortisol and telomere length measurements in ethnically diverse women with breast cancer using culturally sensitive methods.</a> J Community Genet. 8, 75-86 (2017).</li></ol>

头发收集协议的基本步骤包括提供一个例子, 说明将采取多少头发, 显示样本站点 (后涡), 使用单一的应变扭和轻轻拉法, 并确保样品与画家的磁带远离头皮末端。通过轻轻地扭和拉样品, 卷曲的头发被拉长, 头皮端对齐保持。这样可以减少对齐头皮末端、测量和切割样品所需的实验室时间。尽管在先前的研究中使用了变薄剪, 但由于头皮末端排列不均, 而且在准备头发样本进行分析时, 它们并不是切?...

收集唾液, 血液, 或尿液的皮质醇分析有独特的程序性挑战和更大的参与者负担, 因为程序需要多个唾液样本, 静脉穿刺样本, 或24小时尿收集。从头发样本获得皮质醇是一种简单而可接受的替代方法, 使用唾液, 血清, 或尿样1,2。头发皮质醇提供了一个回顾性表示下丘脑-垂体-肾上腺 (HPA) 轴功能随着时间的推移。从头皮上收集的大约3厘米的毛发相当于过去3月1的平均皮质醇释放率。然而, 研究人员在尝试收集不同人群的毛发样本时遇到了独特的挑战。这些挑战包括宗教或精神信仰, 包括揭露头发 (如穆斯林妇女), 头发作为神圣的 (美洲印第安人团体), 或文化规范, 连接头发的标准的美丽和自我形象。
在非洲裔美国人的文化中, 尤其是在女性中, 头皮毛发是他们身体形象3,4的一个非常个人和重要的方面。此外, 历史经验 (例如, 塔斯基吉实验) 在研究过程5中继续助长不信任。一些非洲裔美国人的民俗和文化信仰也表明, 毛发样本可以用于可能造成伤害6,7的仪式。研究人员应该意识到, 当非洲裔美国人的参与者被要求提供毛发样本时, 他们可能面临的潜在挑战。
除了围绕身体图像的问题外, 收集和处理来自非洲裔美国人的毛发样本的具体技术可能与欧洲美国人6,7不同。例如, 非洲裔美国人可能有直, 薄, 卷曲, 古怪, 粗糙, 或这些毛发类型的组合, 每个需要不同的技术来收集和确保足够的头发样本处理。最近对非洲裔美国人青少年样本的研究8以及多种族样本9表明, 在非洲裔美国人的参与下, 为皮质醇水平测量收集毛发是可以接受的。目前, 尚无视觉训练程序, 旨在为研究人员提供在非洲裔美国人收集毛发样本的技能和文化知识。本文的目的是提供一个文化上知情的协议, 以帮助研究人员获得足够的头发样本来自非洲裔美国人的参与者。这一方法是在仔细考虑了与收集来自非洲裔美国人的毛发样本有关的生理、心理和文化特征时制定的。到目前为止, 还没有收集这些样品的视觉方法, 这项议定书将是第一个指南, 详细介绍了从非洲裔美国人成人正确的头发样本收集。为了说明《议定书》执行工作的代表性结果, 从非洲裔美国人那里得到了不确定的数据, 这些人参加了一项以社区为基础的关于65岁以上的慢性压力的研究。来自不同种族背景的男性和女性研究助理都与临床上产生的名单和传单的潜在参与者联系。主要调查员, 谁是非洲裔美国妇女, 在社区中心和高级补贴住房公寓向年长的成年非洲裔美国人介绍了这项研究, 包括对毛发收集方法的描述。

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			<td height="21" style="height:21px;width:216px;">&#160;Salon grade scissors</td>
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			<td style="width:312px;">S-3 550</td>
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			<td style="width:312px;">No catalog number__Goody Stylista Rake Comb&#160;&#160;&#160;</td>
			<td>Link to Company website&#160; http://www.goody.com/Products/Styling_Tools/Goody_Stylista_Rake_Comb?colId=d11c04a1-1dd3-4848-a313-046fc6c109ec&#38;c_id=35a36c88-d359-4104-a53c-dfbfd8452780</td>
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			<td colspan="2">No catalog number_ Goody Start.Style.Finish. Link to company website http://www.goody.com/Products/Styling_Tools/Goody_StartStyleFinish_Combs?colId=d11c04a1-1dd3-4848-a313-046fc6c109ec&#38;c_id=35a36c88-d359-4104-a53c-dfbfd8452780</td>
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			<td style="width:312px;">No catalog number. Kendall Webcol Alcohol Preps</td>
			<td>https://www.buyemp.com/product/kendall-webcol-alcohol-preps</td>
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			<td>Goody Start.Style.Finish. Curl Clips</td>
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			<td>330808</td>
			<td>Office Depot&#174; Brand Clasp Envelopes, 9&#34; x 12&#34;, Brown, Box Of 100</td>
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			<td>Box of 500 Sheets Food Service Interfolded Pop-up foil sheets&#160;</td>
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			<td height="21" style="height:21px;">Pre-weighed 2 ml cryovial</td>
			<td>Wheaton, Millville, NJ, USA</td>
			<td>W985902</td>
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			<td height="21" style="height:21px;">100% isopropanol</td>
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			<td height="21" style="height:21px;">Liquid nitrogen&#160;</td>
			<td style="width:232px;">Ohio State Unversity Stores</td>
			<td>Gas Cylinder Warehouse</td>
			<td>http://osustores.osu.edu/prodservices/liquidnitrogen.aspx</td>
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			<td>McMasterCarr</td>
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			<td height="21" style="height:21px;">Specially milled aluminum cassettes</td>
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			<td height="21" style="height:21px;">HPLC grade methanol</td>
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			<td>https://www.fishersci.com/shop/products/methanol-hplc-fisher-chemical-6/p-216129</td>
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			<td height="21" style="height:21px;">Microcentrifuge tube</td>
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			<td style="width:312px;">22363352</td>
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			<td height="21" style="height:21px;">Cortisol ELISA Kit</td>
			<td>Salimetrics LLC, State College, PA, USA</td>
			<td>1-3002 (5PK 1-3002-5)</td>
			<td>https://www.salimetrics.com/assay-kit/cortisol-salivary-elisa-eia-kit</td>
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		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Centtrifuge for the micorcentrifuge tube</td>
			<td style="width:232px;">Beckman Coulter</td>
			<td style="width:312px;">Microfuge 20 series</td>
			<td>http://centrifugebybeckman.com/?page_id=727/?pi_ad_id=creative?utm_term=microfuge</td>
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collecting hair samples for hair cortisol analysis in african americans

荷尔蒙皮质醇通常在唾液、血清或尿样中进行评估。最近, 皮质醇已经成功地从毛发中提取, 包括人类。头发皮质醇浓度的优势在于它反映了随着时间的推移, 下丘脑-垂体-肾上腺 (HPA) 轴功能的回顾性表现, 就像血红蛋白 A1C 代表血糖控制。然而, 由于少数参与者的文化信仰和护发行为, 获得毛发样本可能具有挑战性。例如, 非洲裔美国人可能不愿意提供样品。此外, 很少有研究人员被训练来收集来自非洲裔美国人的毛发样本。本文的目的是提供一个文化上知情的协议, 以帮助研究人员获得来自非洲裔美国人的头发样本。为了说明本议定书执行工作的代表性结果, 提供了参加基于社区的关于慢性压力的研究的非裔美国人的数据。评估头发练习偏好。参加者通过在理发前展示头发样本的图片使他们感到舒适。单应变扭和轻轻拉法是用来收集大约 30-50 股头发从后顶点区域的头皮。这项议定书将大大改善来自非洲裔美国人的毛发样本的收集。

在我们的研究中, 二十一非洲裔美国人 (平均年龄 = 75;SD = 6.9;85% 女性) 同意参加。四名参加者 (19%) 拒绝提供头发样本, 两人 (9.5%) 不能提供样本, 因为他们的头发太短。共获得和处理15发样品 (表 2)。处理的毛发样本的长度从1厘米到4.8 厘米不等. 技术问题包括样品在头皮末端、纠结或卷曲, 并且画家的磁带被放置在头皮末端。
<p class="jove_content" fo:keep-together.with...

Watch this Scientific Journal Video about Collecting Hair Samples for Hair Cortisol Analysis in African Americans at JoVE.com

Collecting Hair Samples for Hair Cortisol Analysis in African Americans

头发皮质醇浓度分析提供了替代传统的皮质醇措施;然而, 要收集来自非洲裔美国人的毛发样本, 科学家需要有文化上的了解和能力。本议定书的目的是展示一种文化上消息灵通的技术来收集来自非洲裔美国人的皮质醇分析的毛发样本。

在非洲裔美国人中收集头发皮质醇分析样本

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收集，分离，和人类宫颈样品的流式细胞仪分析

Despite the public health importance of mucosal pathogens (including HIV), relatively little is known about mucosal immunity, particularly at the female ...

Carotid Artery Infusions for Pharmacokinetic and Pharmacodynamic Analysis of Taxanes in Mice

颈动脉输液的紫杉烷类化合物在小鼠体内的药代动力学和药效学分析

When proposing the use of a drug, drug combination, or drug delivery into a novel system, one must assess the pharmacokinetics of the drug in the study ...

Rapid Fractionation and Isolation of Whole Blood Components in Samples Obtained from a Community-based Setting

快速分馏和全血的成分的分离样品中从社区为基础的设置获得

Collection and processing of whole blood samples in a non-clinical setting offers a unique opportunity to evaluate community-dwelling individuals both ...

Optimized LC-MS/MS Method for the High-throughput Analysis of Clinical Samples of Ivacaftor, Its Major Metabolites, and Lumacaftor in Biological Fluids of Cystic Fibrosis Patients

经优化的 LC/ms 方法对囊性纤维化患者 Ivacaftor 及其主要代谢物和 Lumacaftor 的高通量分析

Defects in the cystic fibrosis trans-membrane conductance regulator (CFTR) are the cause of cystic fibrosis (CF), a disease with life-threatening ...

Sampling Strategies and Processing of Biobank Tissue Samples from Porcine Biomedical Models

猪生物医学模型生物库组织样品的取样策略与处理

In translational medical research, porcine models have steadily become more popular. Considering the high value of individual animals, particularly of ...

High-Throughput Analysis of Optical Mapping Data Using ElectroMap

使用电子地图的光映射数据的高通量分析

Optical mapping is an established technique for high spatio-temporal resolution study of cardiac electrophysiology in multi-cellular preparations. Here we ...

Validated LC-MS/MS Panel for Quantifying 11 Drug-Resistant TB Medications in Small Hair Samples

经过验证的LC-MS/MS面板，用于在小头发样本中量化11种耐药结核病药物

Drug resistant-tuberculosis (DR-TB) is a growing public health threat, and assessment of therapeutic drug levels may have important clinical benefits. ...

On-Site Sampling and Extraction of Brain Tumors for Metabolomics and Lipidomics Analysis

代谢组学和利皮多米分析脑肿瘤的现场采样与提取

Despite the variety of tools available for cancer diagnosis and classification, methods that enable fast and simple characterization of tumors are still ...