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DOI :
10.3791/57298-v
May 4th, 2018
Chapters
0:04
Title
1:26
Growth of PA14NR Set Master Copy on LB Agar Plates
5:02
Growth of the PA14NR Set Master Copy in LB Liquid Broth
7:01
Transfer of PA14NR Set Overnight Cultures into Destination Plates
9:43
Results: Protocol Generates High-quality Copies of the PA14NR Set Mutant Library
10:38
Conclusion
铜绿假单胞菌感染会导致易受害主机发病率显著。非冗余座子插入突变库的绿脓杆菌菌株 PA14, 指定为 PA14NR 集, 促进分析的基因功能在许多过程中。这里提出了一个协议, 以生成高质量的副本 PA14NR 集突变库。
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