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DOI :
10.3791/57298-v
May 4th, 2018
Chapters
0:04
Title
1:26
Growth of PA14NR Set Master Copy on LB Agar Plates
5:02
Growth of the PA14NR Set Master Copy in LB Liquid Broth
7:01
Transfer of PA14NR Set Overnight Cultures into Destination Plates
9:43
Results: Protocol Generates High-quality Copies of the PA14NR Set Mutant Library
10:38
Conclusion
緑膿菌感染症は、脆弱なホストで重大な合併症を引き起こします。膿ひずみ PA14、PA14NR を設定すると、指定の非冗長トランスポゾン挿入変異ライブラリー多数プロセスの遺伝子機能の解析が容易になります。ここに提示 PA14NR セット変異ライブラリーの高品質のコピーを生成するためのプロトコルです。
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