Name: phase contrast magnetic resonance imaging in the rat common carotid artery
Uploaded: 2018-09-05T15:30:00
Description: Watch this Scientific Journal Video about Phase Contrast Magnetic Resonance Imaging in the Rat Common Carotid Artery at JoVE.com

这项工作得到了台湾科学技术部授予的 MOST-105-2314-B-039-044-MY2 的赠款支持。

中国医科大学的机构护理和使用委员会 (IACUC) 批准了所有的程序。
1. 动物准备和监测
<ol><li>在开始进行 MRI 扫描的动物准备之前, 在扫描仪室外留下所有磁性敏感物品, 如钱包、钥匙、信用卡等。</li>
	<li>最初麻醉鼠 (2 月大的雄性大 (SD) 鼠, 280–350 g) 在一个感应箱中使用5% 异氟醚 (ISO) 和氧气 (2 升/分钟) 的混合物, 为3–5分钟, 必要时。</li>
	<li>当动物处于卧位时, 没有对...

<ol>
	<li>Zakszewski, E., Schmit, B., Kurpad, S., Budde, M. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Diffusion+imaging+in+the+rat+cervical+spinal+cord.">Diffusion imaging in the rat cervical spinal cord.</a> J Vis Exp. (98), (2015).</li><li>Wise, R. G., Al-Shafei, A. I., Carpenter, T. A., Hall, L. D., Huang, C. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Simultaneous+measurement+of+blood+and+myocardial+velocity+in+the+rat+heart+by+phase+contrast+MRI+using+sparse+q-space+sampling.">Simultaneous measurement of blood and myocardial velocity in the rat heart by phase contrast MRI using sparse q-space sampling.</a> J Magn Reson Imaging. 22 (5), 614-627 (2005).</li><li>Skardal, K., Espe, E. K., Zhang, L., Aronsen, J. M., Sjaastad, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Three-Directional+Evaluation+of+Mitral+Flow+in+the+Rat+Heart+by+Phase-Contrast+Cardiovascular+Magnetic+Resonance.">Three-Directional Evaluation of Mitral Flow in the Rat Heart by Phase-Contrast Cardiovascular Magnetic Resonance.</a> PLoS One. 11 (3), e0150536 (2016).</li><li>Peng, S. L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Phase-contrast+magnetic+resonance+imaging+for+the+evaluation+of+wall+shear+stress+in+the+common+carotid+artery+of+a+spontaneously+hypertensive+rat+model+at+7T:+Location-specific+change,+regional+distribution+along+the+vascular+circumference,+and+reproducibility+analysis.">Phase-contrast magnetic resonance imaging for the evaluation of wall shear stress in the common carotid artery of a spontaneously hypertensive rat model at 7T: Location-specific change, regional distribution along the vascular circumference, and reproducibility analysis.</a> Magn Reson Imaging. 34 (5), 624-631 (2016).</li><li>Yu, H. Y., Peng, H. H., Wang, J. L., Wen, C. Y., Tseng, W. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Quantification+of+the+pulse+wave+velocity+of+the+descending+aorta+using+axial+velocity+profiles+from+phase-contrast+magnetic+resonance+imaging.">Quantification of the pulse wave velocity of the descending aorta using axial velocity profiles from phase-contrast magnetic resonance imaging.</a> Magn Reson Med. 56 (4), 876-883 (2006).</li><li>Peng, S. L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Optimization+of+phase-contrast+MRI+for+the+quantification+of+whole-brain+cerebral+blood+flow.">Optimization of phase-contrast MRI for the quantification of whole-brain cerebral blood flow.</a> J Magn Reson Imaging. 42 (4), 1126-1133 (2015).</li><li>Peng, S. L., Shih, C. T., Huang, C. W., Chiu, S. C., Shen, W. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Optimized+analysis+of+blood+flow+and+wall+shear+stress+in+the+common+carotid+artery+of+rat+model+by+phase-contrast+MRI.">Optimized analysis of blood flow and wall shear stress in the common carotid artery of rat model by phase-contrast MRI.</a> Sci Rep. 7 (1), 5253 (2017).</li><li>Bozgeyik, Z., Berilgen, S., Ozdemir, H., Tekatas, A., Ogur, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Evaluation+of+the+effects+of+sildenafil+citrate+(viagra)+on+vertebral+artery+blood+flow+in+patients+with+vertebro-basilar+insufficiency.">Evaluation of the effects of sildenafil citrate (viagra) on vertebral artery blood flow in patients with vertebro-basilar insufficiency.</a> Korean J Radiol. 9 (6), 477-480 (2008).</li><li>Swampillai, J., Rakebrandt, F., Morris, K., Jones, C. J., Fraser, A. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Acute+effects+of+caffeine+and+tobacco+on+arterial+function+and+wave+travel.">Acute effects of caffeine and tobacco on arterial function and wave travel.</a> Eur J Clin Invest. 36 (12), 844-849 (2006).</li><li>Neff, K. W., Horn, P., Schmiedek, P., Duber, C., Dinter, D. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=2D+cine+phase-contrast+MRI+for+volume+flow+evaluation+of+the+brain-supplying+circulation+in+moyamoya+disease.">2D cine phase-contrast MRI for volume flow evaluation of the brain-supplying circulation in moyamoya disease.</a> AJR Am J Roentgenol. 187 (1), W107-W115 (2006).</li><li>Stalder, A. F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Quantitative+2D+and+3D+phase+contrast+MRI:+optimized+analysis+of+blood+flow+and+vessel+wall+parameters.">Quantitative 2D and 3D phase contrast MRI: optimized analysis of blood flow and vessel wall parameters.</a> Magn Reson Med. 60 (5), 1218-1231 (2008).</li><li>Dall'Armellina, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Improved+method+for+quantification+of+regional+cardiac+function+in+mice+using+phase-contrast+MRI.">Improved method for quantification of regional cardiac function in mice using phase-contrast MRI.</a> Magn Reson Med. 67 (2), 541-551 (2012).</li><li>Peng, S. L., Ravi, H., Sheng, M., Thomas, B. P., Lu, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Searching+for+a+truly+&amp;#34;iso-metabolic&amp;#34;+gas+challenge+in+physiological+MRI.">Searching for a truly &amp;#34;iso-metabolic&amp;#34; gas challenge in physiological MRI.</a> J Cereb Blood Flow Metab. 37 (2), 715-725 (2017).</li><li>Liu, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Quantitative+assessment+of+global+cerebral+metabolic+rate+of+oxygen+(CMRO2)+in+neonates+using+MRI.">Quantitative assessment of global cerebral metabolic rate of oxygen (CMRO2) in neonates using MRI.</a> NMR Biomed. 27 (3), 332-340 (2014).</li><li>Xu, F., Ge, Y., Lu, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Noninvasive+quantification+of+whole-brain+cerebral+metabolic+rate+of+oxygen+(CMRO2)+by+MRI.">Noninvasive quantification of whole-brain cerebral metabolic rate of oxygen (CMRO2) by MRI.</a> Magn Reson Med. 62 (1), 141-148 (2009).</li><li>Lotz, J., Meier, C., Leppert, A., Galanski, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cardiovascular+flow+measurement+with+phase-contrast+MR+imaging:+basic+facts+and+implementation.">Cardiovascular flow measurement with phase-contrast MR imaging: basic facts and implementation.</a> Radiographics. 22 (3), 651-671 (2002).</li><li>Pelc, N. J., Herfkens, R. J., Shimakawa, A., Enzmann, D. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Phase+contrast+cine+magnetic+resonance+imaging.">Phase contrast cine magnetic resonance imaging.</a> Magn Reson Q. 7 (4), 229-254 (1991).</li><li>Kim, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Accelerated+phase-contrast+cine+MRI+using+k-t+SPARSE-SENSE.">Accelerated phase-contrast cine MRI using k-t SPARSE-SENSE.</a> Magn Reson Med. 67 (4), 1054-1064 (2012).</li><li>Valvano, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Accelerating+4D+flow+MRI+by+exploiting+low-rank+matrix+structure+and+hadamard+sparsity.">Accelerating 4D flow MRI by exploiting low-rank matrix structure and hadamard sparsity.</a> Magn Reson Med. 78 (4), 1330-1341 (2017).</li></ol>

MRI 是一种非侵入性和纵向评价血流的综合方法。本文提出了一种用于大鼠共同评价的 PC 磁共振成像的协议。本程序易于在任何动物 MRI 扫描仪中执行, 并显示良好的重现性。
PC MRI 技术在人类10、11以及动物研究中得到了越来越多的普及, 4、12。在这些研究中, 彭等的结果。<sup class="x...

磁共振成像 (MRI) 是一种多功能的方法, 提供了有关内部身体结构和生理学的详细信息, 并越来越多地用于临床诊断和前期动物研究。动物模型是至关重要的, 以更好地了解相当多的临床意义1。由于动物模型在麻醉要求和生理参数方面与人类有很大的不同, 因此对这种动物的 MRI 程序进行优化具有重要意义。
磁共振成像 (mri) 是一种特殊类型的 mri, 利用流动旋转的速度来量化与流动相关的参数, 如血流。借助于 PC MRI, 利用动物模型对主要动脉中的流型进行映射可以帮助揭示心血管病理2。此外, PC MRI 可无创监测病理生理学情况下血液流动的内在变动3。这些观察表明, PC MRI 是一种有价值的方法, 可用于人类心血管疾病的动物模型。
在本报告中, 我们描述了一种定量的血流量在颈总动脉 (共同评估) 的大鼠。两种共同国家评估为头颈部提供含氧血, 颈动脉疾病是中风的主要原因。因此, 检测早期病理在共同国家评估中是至关重要的。这一过程的持续时间约为15分钟, 并可能适用于条件与血流动力学改变, 如动脉粥样硬化或中风。

<table border="0" cellpadding="0" cellspacing="0" width="875">
	<tbody>
		<tr height="40">
			<td height="40" style="height:40px;width:216px;">7T small animal MRI system</td>
			<td style="width:123px;">Bruker</td>
			<td style="width:344px;"></td>
			<td style="width:193px;"></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;">Isoflurane&#160;</td>
			<td>Baxter</td>
			<td>1001936040</td>
			<td>anesthetic</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;">ECG lead&#160;</td>
			<td>3M</td>
			<td>2269T</td>
			<td style="width:193px;"></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;">Matlab</td>
			<td>MathWorks</td>
			<td></td>
			<td>sofeware for image processing</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;">Monitoring and gating system</td>
			<td>SA instruments, Inc</td>
			<td>Model 1030</td>
			<td style="width:193px;"></td>
		</tr>
	</tbody>
</table>

phase contrast magnetic resonance imaging in the rat common carotid artery

相衬磁共振成像 (PC MRI) 是一种无创的方法, 可以量化流量相关参数, 如血流。以前的研究表明, 异常的血流可能与系统性血管危险有关。因此, PC MRI 可以促进从心血管疾病动物模型获得的数据的翻译到相关的临床调查。在本报告中, 我们描述了用电影门控电脑 MRI 测量大鼠颈总动脉血流量的方法, 并讨论了相关的分析方法。这一程序可以在活体, 麻醉动物, 不需要安乐死后的程序。所提出的扫描参数产生可重复测量的血液流量, 表明了优良的重现性的结果。本文所描述的 PC MRI 程序可用于药理试验、病理生理学评估和脑血流动力学评价。

正确的切片几何是确保 PC MRI 实验成功的关键。准确的图像平面定位产生一个 "圆形" 的动脉形状 (图 3a), 随着角度的增加,即, 当它与动脉垂直较少时, 产生的动脉几何变成卵球形, 导致较大的部分体积效果 (图 3b)。严重的局部体积效应会导致对血流的高估6,7。因此, 如果动脉形状...

Watch this Scientific Journal Video about Phase Contrast Magnetic Resonance Imaging in the Rat Common Carotid Artery at JoVE.com

Phase Contrast Magnetic Resonance Imaging in the Rat Common Carotid Artery

本程序的总体目标是采用无创相磁共振成像技术对大鼠颈总动脉血流进行测量。

大鼠颈总动脉的相位对比磁共振成像

Phase contrast magnetic resonance imaging (PC-MRI) is a noninvasive approach that can quantify flow-related parameters such as blood flow. Previous ...

Research

JoVE Journal

Medicine

Magnetic Resonance Derived Myocardial Strain Assessment Using Feature Tracking

磁共振衍生的心肌劳损评估使用特征跟踪

Purpose: An accurate and practical method to measure parameters like strain in myocardial tissue is of great clinical value, since it has been shown, that ...

科研

医学

Training a Sophisticated Microsurgical Technique: Interposition of External Jugular Vein Graft in the Common Carotid Artery in Rats

培养一个精密的显微外科技术在大鼠颈总动脉，颈外静脉移植：介入

Neointimal hyperplasia is one the primary causes of stenosis in arterialized veins that are of great importance in arterial coronary bypass surgery, in ...

Multi-modal Imaging of Angiogenesis in a Nude Rat Model of Breast Cancer Bone Metastasis Using Magnetic Resonance Imaging, Volumetric Computed Tomography and Ultrasound

利用磁共振成像，容积电脑断层扫描及超音波的裸鼠乳腺癌骨转移大鼠模型血管生成的多模态成像

Angiogenesis is an essential feature of cancer growth and metastasis formation. In bone metastasis, angiogenic factors are pivotal for tumor cell ...

A Murine Model of Stent Implantation in the Carotid Artery for the Study of Restenosis

颈动脉支架植入再狭窄的研究的小鼠模型

Despite  the considerable progress made in the stent development in the last decades,  cardiovascular diseases remain the main cause of death in western ...

Adult Mouse Venous Hypertension Model: Common Carotid Artery to External Jugular Vein Anastomosis.

成年小鼠静脉高压型号：颈总动脉，以颈外静脉吻合。

The understanding of the pathophysiology of brain arteriovenous malformations and arteriovenous fistulas has improved thanks to animal models. A rat model ...

Vascular Balloon Injury and Intraluminal Administration in Rat Carotid Artery

血管球囊损伤和腔内管理在大鼠颈动脉

The carotid artery balloon injury model in rats has been well established for over two decades. It remains an important method to study the molecular and ...

Dynamic Contrast Enhanced Magnetic Resonance Imaging of an Orthotopic Pancreatic Cancer Mouse Model

原位胰腺癌小鼠模型的动态对比增强磁共振成像

Dynamic contrast enhanced magnetic resonance imaging (DCE-MRI) has been limitedly used for orthotopic pancreatic tumor xenografts due to severe ...

Performing Permanent Distal Middle Cerebral with Common Carotid Artery Occlusion in Aged Rats to Study Cortical Ischemia with Sustained Disability

执行永久远端大脑中与老年大鼠颈总动脉闭塞研究皮层缺血具有持续残疾

Stroke typically occurs in elderly people with a range of comorbidities including carotid (or other arterial) atherosclerosis, high blood pressure, ...

Cardiac Magnetic Resonance Imaging at 7 Tesla

7号特斯拉的心脏磁共振成像

CMR at an ultra-high field (magnetic field strength B0&#160;&#8805; 7 Tesla) benefits from the signal-to-noise ratio (SNR) advantage inherent at higher ...

In Vivo Gene Transfer to the Rabbit Common Carotid Artery Endothelium

In Vivo Gene Transfer to the Rabbit Common Carotid Artery Endothelium

体内兔颈总动脉内皮的基因转移

The goal of this method is to introduce a transgene into the endothelium of isolated segments of both rabbit common carotid arteries. The method achieves ...