Name: phase contrast magnetic resonance imaging in the rat common carotid artery
Uploaded: 2018-09-05T15:30:00
Description: Watch this Scientific Journal Video about Phase Contrast Magnetic Resonance Imaging in the Rat Common Carotid Artery at JoVE.com

Dette arbejde blev støttet af tilskud fra ministeriet for videnskab og teknologi, Taiwan, under tilskud antal MOST-105-2314-B-039-044-MY2.

Institutionel pleje og brug udvalg (IACUC) af Kina medicinske universitet godkendt alle procedurer.
1. animalsk forberedelse og overvågning
<ol><li>Forlade alle magnetisk modtagelige objekter såsom punge, nøgler, kreditkort m.v. udenfor scanner værelset før påbegyndelse animalske forberedelse til MR scanning.</li>
	<li>I første omgang bedøver rotte (2-måned-forhenværende mandlige Sprague-Dawley (SD) rotte, 280-350 g) i en induktion boks ved hjælp af en blanding af 5% isof...

<ol>
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PC-Mr er en omfattende strategi for non-invasiv og langsgående vurdering af blodgennemstrømningen. Vi præsenterer en protokol til at udføre PC-MRI af rotte CCA. Denne procedure er let at udføre i ethvert dyr MR scanner og viser god reproducerbarhed.
PC-Mr-teknik har opnået stigende popularitet i menneskelige 10,11 samt dyreforsøg 4,12. Blandt disse undersøgelser, result...

Magnetisk resonans imaging (MR) er en alsidig tilgang, der giver detaljerede oplysninger om indre organ strukturer og fysiologi, og er i stigende grad anvendes til klinisk diagnose og i prækliniske dyreforsøg. Dyremodeller er afgørende for forståelsen af betydelige kliniske implikation 1. Som dyremodeller afviger betydeligt fra mennesker med hensyn til anæstesi krav og fysiologiske parametre, antager optimering af Mr procedurer for sådanne dyr betydning.
Fase kontrast MRI (PC-MRI) er en specialiseret type af MRI, som bruger hastigheden af strømmende spins til at kvantificere flow-relaterede parametre såsom blodgennemstrømning. Med PC-MRI, kan kortlægning flow mønstre i de store arterier, ved hjælp af dyremodeller hjælpe kaste lys på hjerte-kar-sygdomme 2. Derudover kan PC-Mr ikke-invasivt overvåge de iboende vekslen i blodgennemstrømningen i patofysiologiske forhold 3. Disse observationer tyder på, at PC-Mr er en værdifuld fremgangsmåde, der kan bruges i dyremodeller for humane hjerte-kar-sygdomme.
I denne betænkning beskriver vi en metode til kvantificering af blodgennemstrømningen i den fælles halspulsåren (CCA) af rotter. De to CCAs levere hoved og hals med iltet blod, og halspulsåren sygdom er en væsentlig årsag til slagtilfælde. Derfor afsløre tidlige patologi i CCA er afgørende. Denne procedure har en varighed på ca. 15 min. og potentielt kan anvendes til tilstande med Hæmodynamik ændringer, sådan åreforkalkning eller slagtilfælde.

<table border="0" cellpadding="0" cellspacing="0" width="875">
	<tbody>
		<tr height="40">
			<td height="40" style="height:40px;width:216px;">7T small animal MRI system</td>
			<td style="width:123px;">Bruker</td>
			<td style="width:344px;"></td>
			<td style="width:193px;"></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;">Isoflurane&#160;</td>
			<td>Baxter</td>
			<td>1001936040</td>
			<td>anesthetic</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;">ECG lead&#160;</td>
			<td>3M</td>
			<td>2269T</td>
			<td style="width:193px;"></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;">Matlab</td>
			<td>MathWorks</td>
			<td></td>
			<td>sofeware for image processing</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;">Monitoring and gating system</td>
			<td>SA instruments, Inc</td>
			<td>Model 1030</td>
			<td style="width:193px;"></td>
		</tr>
	</tbody>
</table>

phase contrast magnetic resonance imaging in the rat common carotid artery

Fase kontrast magnetisk resonans imaging (PC-Mr) er en noninvasiv tilgang, der kan kvantificere flow-relaterede parametre såsom blodgennemstrømning. Tidligere undersøgelser har vist, at unormal blodgennemstrømning kan være forbundet med systemisk vaskulær risiko. Således kan PC-Mr lette oversættelsen af data indhentet fra dyremodeller for hjerte-kar-sygdomme til relevante kliniske undersøgelser. I denne betænkning, vi beskrive proceduren for måling af blodgennemstrømningen i den fælles halspulsåren (CCA) af rotter benytter cine-gated PC-MRI og diskutere relevante analysemetoder. Denne procedure kan udføres i et levende, bedøvede dyr og kræver ikke aktiv dødshjælp efter indgrebet. De foreslåede scanningsparametre udbytte gentagelig målinger for blodgennemstrømning, der angiver fremragende reproducerbarhed af resultaterne. PC-Mr fremgangsmÃ ¥ den i denne artikel kan bruges til farmakologisk testning, patofysiologiske vurdering og cerebral Hæmodynamik evaluering.

Korrekte skive geometri er afgørende for at sikre succes for PC-Mr eksperiment. Nøjagtige billede fly positionering udbytter en "runde" arterie figur (figur 3a), og som vinkling stiger, dvs., når det er mindre vinkelret på arterie, den resulterende arterie geometri bliver æggeformet, fører til større delvis volumen effekter (figur 3b). Svær delvis volumen effekter ville føre til overvurdering af blod flow <sup c...

Watch this Scientific Journal Video about Phase Contrast Magnetic Resonance Imaging in the Rat Common Carotid Artery at JoVE.com

Phase Contrast Magnetic Resonance Imaging in the Rat Common Carotid Artery

Det overordnede mål med denne procedure er at måle blodgennemstrømningen i rotte fælles halspulsåren ved hjælp af noninvasive fase kontrast magnetisk resonans.

Fase kontrast magnetisk resonans i rotte fælles halspulsåren

Phase contrast magnetic resonance imaging (PC-MRI) is a noninvasive approach that can quantify flow-related parameters such as blood flow. Previous ...

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