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DOI :
10.3791/57311-v
June 9th, 2018
Chapters
0:04
Title
1:30
In Utero Electroporation
5:16
Preparation of Cryosections from Electroporated Cerebella
6:22
Immunostaining the Cerebellar Cryosections
7:38
Results: Immunostained Cerebella from Rosa26-CAG-LSL-Cas9-P2A-EGFP Mouse
8:48
Conclusion
ノックアウト動物を用いた遺伝子の機能喪失従来は、高価で時間のかかる多くの場合されています。CRISPR 媒介体細胞突然変異誘発のエレクトロポレーション ベースは遺伝子の生体内の機能を理解するための強力なツールです。小脳の細胞増殖にノックアウトの表現型を解析する手法を報告する.
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