Name: fabricating high-viscosity droplets using microfluidic capillary device with phase-inversion co-flow structure
Uploaded: 2018-04-17T14:00:00
Description: Watch this Scientific Journal Video about Fabricating High-viscosity Droplets using Microfluidic Capillary Device with Phase-inversion Co-flow Structure at JoVE.com

这项工作得到了中国国家自然科学基金 (51420105006 和51322501号) 的支持。我们感谢丹尼尔对高粘度思想的有益讨论。

1. 生产一种相反转的共流毛细管装置, 用于观察含直径为500微米的高粘度水滴的生成过程。
注: 此处使用的方形外管用于拍摄高粘度水滴的生成过程的图像。如果不需要拍摄图像, 则可以根据协议步骤2对设备进行简化版本。
<ol><li>
为毛细管装置的组装准备三个不同尺寸的玻璃管。
	<ol>
<li>取一个方形的玻璃管, 内部尺寸为1.05 毫米, 并切割一条管子的长度为4厘米?...

<ol>
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反相共流装置提供了一种简单、直接的方法, 能产生单分散高粘度水滴。该装置与普通共流装置具有相似的结构, 因为基本的共流结构由插入在中间管中的内管组成, 其出口与出口油管相连。然而, 相逆共流装置与普通共流装置之间存在两个主要区别, 即产生粘度为η > 1 Pa·s 的高粘度水滴。
首先, 在常用的共流装置中, 采用锥形尖端的内管, 而直内管可用于相倒置的共流装...

水滴的产生正在成为各种应用的关键技术, 如药物输送, 材料合成, 3D 生物打印, 细胞化验, 食品工程 1, 2, 3, 4,5,6. 带 T 形连接7、8、co 流1、9或流聚焦10、11结构的微流控设备广泛用于生成单分散单乳液滴。选择一个更粘性的连续相将有助于形成水滴12, 并且连续和分散的流体的粘度通常低于 0.1 Pas 在液滴微流体 13.然而, 在许多应用中, 分散相的粘度可能比水高几倍, 例如甘油14、含有纳米粒子15、蛋白质16或聚合物17的溶液。,18,19, 而在微流控设备中, 很难直接从稳定的滴水状态11中的高粘度流体中实现单分散液滴, 特别是对于具有η > 1 Pa·s14的粘度的流体.,17,18,19。此外, 还报告了13、18 , 认为液滴形成的典型微流控方法要求流体具有相对较低的粘度和适度的界面张力, 形成稳定滴水中的均匀水滴。政权。
对于具有稍大于 0.1 Pas 的粘度的分散相位, 有几种可能的方法可以通过典型的 T 连接、共流或流聚焦微流控装置来促进液滴的形成: (1) 降低分散的粘度在挥发性溶剂11,20中稀释它的相位;(2) 通过增加连续相位1,11的粘度, 减少分散到连续的粘度比;(3) 将分散相的流速降低到极低的值, 同时保持高连续到分散的流量比率14,19。但是, 这些方法对于粘度高得多的流体来说是不切实际的, 因为它们会显著降低生产速率, 同时显著提高挥发性溶剂或连续相的消耗。在百货商店, 据报道, 一些高粘度聚合物溶液与η > 1 Pa·s 仍然没有分解成水滴与上述方法17,19。
还有几种改进的微流控装置的设计, 将第三阶段的流体引入到系统中, 从而促进了高粘度滴的产生。创新包括: 引入气泡将喷射螺纹切割成水滴21, 一种具有中等粘度的不溶做伴流体, 引入 dipsersed 相和连续相18之间的中间相, 并微反应器介绍了从两个低粘度前体生成高粘度的小水滴 21, 22, 23.然而, 随着这个过程中的一个流体越来越多, 这个系统变得更加复杂, 而这些装置通常在比生成单乳液滴的典型装置更窄的流动机制中工作。
为了直接从高粘度流体中产生单分散液滴与η > 1 Pa·s, 研究了表面控相反演方法的24。由于低黏度水滴的产生比高粘度水滴容易得多12, 高粘度连续相的细长低黏度水滴首先使用典型的共流结构产生, 然后被分解为在共流结构的下游表面润湿性的变化。释放的低粘度流体将下游高粘度流体封装成水滴, 从而完成了相位反演。根据相变机理, 单分散高粘度液滴可根据典型的共流装置产生, 而该共流装置的出口被处理为由低粘度流体润湿, 然后连接到更宽的管24 ,25。

<table>
	<tbody>
		<tr>
			<td>VitroTubes Glass Tubing</td>
			<td>VitroCom</td>
			<td>8240</td>
			<td>Square - Miniature Hollow Glass Tubing, I.D.=0.4mm, OD=0.8mm</td>
		</tr>
		<tr>
			<td>VitroTubes Glass Tubing</td>
			<td>VitroCom</td>
			<td>CV2033</td>
			<td>Round - Miniature Hollow Glass Tubing, I.D.=0.2mm, O.D.=0.33mm</td>
		</tr>
		<tr>
			<td>VitroTubes Glass Tubing</td>
			<td>VitroCom</td>
			<td>CV1017</td>
			<td>Round - Miniature Hollow Glass Tubing, I.D.=0.1mm, O.D.=0.17mm</td>
		</tr>
		<tr>
			<td>VitroTubes Glass Tubing</td>
			<td>VitroCom</td>
			<td>Q14606</td>
			<td>Square - Miniature Hollow Glass Tubing, I.D.=1.05mm+0.1/-0, OD=1.5mm</td>
		</tr>
		<tr>
			<td>Standard Glass Capillaries</td>
			<td>WPI</td>
			<td>1B100-6</td>
			<td>Round - Glass Tubing, I.D.=0.58mm, O.D.=1.00mm</td>
		</tr>
		<tr>
			<td>Glycerol</td>
			<td>Sinopharm Chemical Reagent Beijing</td>
			<td>10010618</td>
			<td></td>
		</tr>
		<tr>
			<td>Paraffin Liquid</td>
			<td>Sinopharm Chemical Reagent Beijing</td>
			<td>30139828</td>
			<td></td>
		</tr>
		<tr>
			<td>Poly(vinyl alcohol), PVA-124</td>
			<td>Sinopharm Chemical Reagent Beijing</td>
			<td>30153084</td>
			<td></td>
		</tr>
		<tr>
			<td>Span 80</td>
			<td>Sigma-Aldrich</td>
			<td>85548</td>
			<td></td>
		</tr>
		<tr>
			<td>Starch</td>
			<td>Sigma-Aldrich</td>
			<td>S9765</td>
			<td></td>
		</tr>
		<tr>
			<td>Trichloro(octadecyl)silane</td>
			<td>Sigma-Aldrich</td>
			<td>104817</td>
			<td></td>
		</tr>
		<tr>
			<td>Toluidine Blue O</td>
			<td>Sigma-Aldrich</td>
			<td>T3260</td>
			<td></td>
		</tr>
		<tr>
			<td>Honey</td>
			<td></td>
			<td></td>
			<td>Chaste tree honey, common food product purchased from supermarket</td>
		</tr>
		<tr>
			<td>DEVCON 5 Minute Epoxy</td>
			<td>ITW&#160;</td>
			<td></td>
			<td>Epoxy glue</td>
		</tr>
		<tr>
			<td>Blunt Tip Stainless Steel Dispensing Needles (Luer Lock)</td>
			<td>Suzhou Lanbo Needle, China</td>
			<td>LTA820050</td>
			<td>20G x 1/2&#34;&#160;</td>
		</tr>
		<tr>
			<td>Tungsten/Carbide Scriber</td>
			<td>Ullman</td>
			<td>1830</td>
			<td>For cutting glass tubing</td>
		</tr>
		<tr>
			<td>Microscope Slides</td>
			<td>Sail Brand</td>
			<td>7101</td>
			<td>76.2 mm x 25.4 mm, Thickness 1 - 1.2 mm</td>
		</tr>
		<tr>
			<td>Polyethylene Tubing</td>
			<td>Scientific Commodities</td>
			<td>BB31695-PE/5</td>
			<td>I.D. = 0.86 mm, O.D. = 1.32 mm</td>
		</tr>
		<tr>
			<td>Syringe Pumps</td>
			<td>Longer Pump, China</td>
			<td>LSP01-1A</td>
			<td>3 pumps needed for the experiments</td>
		</tr>
	</tbody>
</table>

fabricating high-viscosity droplets using microfluidic capillary device with phase-inversion co-flow structure

高粘度的单分散液滴的产生一直是液滴微流体的挑战。在这里, 我们演示了一个相逆共流装置, 以产生均匀的高粘度液滴在低粘度流体。微流控毛细管装置有一个共同的共流结构, 其出口连接到一个更宽的管。低粘度流体的细长滴液首先由高粘度流体在共流结构中封装。当细长的低粘度水滴流过出口时, 被低粘度流体所润湿, 然后通过低粘度水滴与出口尖端的粘附来诱导相反转, 从而导致随后的反高粘度流体的封装。通过将低粘度流体的流速比转化为高粘度流体, 可以调节产生的高粘度水滴的大小。我们展示了几个典型的例子, 产生高粘度的水滴, 粘度高达 11.9 Pas, 如甘油, 蜂蜜, 淀粉和聚合物溶液。该方法提供了一种简单而直接的方法来生成单分散高粘度滴, 可用于各种液滴的应用, 如材料合成, 药物输送, 细胞化验, 生物工程和食品工程。

采用相反转、共流结构的微流控毛细管装置, 设计成单分散水性高粘度水滴, 如图 1A所示。在图 1中, 高粘度水相是甘油, 其黏度为ηw = 1.4 Pas;低粘度油相为液体石蜡, 其粘度为ηo = 0.029 Pas;两个阶段之间的表面张力为γ = 27.7 锰/米。在中间管, 拉长的油滴可以由甘油封装在一个良好控制的滴水模式<sup cla...

Watch this Scientific Journal Video about Fabricating High-viscosity Droplets using Microfluidic Capillary Device with Phase-inversion Co-flow Structure at JoVE.com

Fabricating High-viscosity Droplets using Microfluidic Capillary Device with Phase-inversion Co-flow Structure

反相共流微流控毛细管装置制备高粘度水滴的方法

本文展示了一种反相共流装置, 以产生单分散高粘度在 1 Pas 以上的液滴, 在液滴微流体中难以实现。

The generation of monodisperse droplets with high viscosity has always been a challenge in droplet microfluidics. Here, we demonstrate a phase-inversion ...

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Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR

Copper (I) binding by metallochaperone transport proteins prevents copper oxidation and release of the toxic ions that may participate in harmful redox ...

Capillary Electrophoresis to Monitor Peptide Grafting onto Chitosan Films in Real Time

毛细管电泳监视肽接枝壳聚糖膜实时

Free-solution capillary electrophoresis (CE) separates analytes, generally charged compounds in solution through the application of an electric field. ...

Using Capillary Electrophoresis to Quantify Organic Acids from Plant Tissue: A Test Case Examining Coffea arabica Seeds

Using Capillary Electrophoresis to Quantify Organic Acids from Plant Tissue: A Test Case Examining Coffea arabica Seeds

利用毛细管电泳从植物组织量化有机酸：一个测试用例检查<em&gt;小粒种咖啡</em&gt;种子

Carboxylic acids are organic acids containing one or more terminal carboxyl (COOH) functional groups. Short chain carboxylic acids (SCCAs; carboxylic ...

Monitoring the Effects of Illumination on the Structure of Conjugated Polymer Gels Using Neutron Scattering

利用中子散射监测光照对共轭高分子凝胶结构的影响

We demonstrate a protocol to effectively monitor the gelation process of a high concentration solution of conjugated polymer both in the presence and ...

Preparation of Hollow Polystyrene Particles and Microcapsules by Radical Polymerization of Janus Droplets Consisting of Hydrocarbon and Fluorocarbon Oils

由烃类和氟碳油组成的同液滴的自由基聚合制备空心聚苯乙烯颗粒和微胶囊

In this article, we have demonstrated a method for producing hollow particles and microcapsules using oil droplets consisting of hydrocarbon oil (styrene) ...

Probing the Structure and Dynamics of Interfacial Water with Scanning Tunneling Microscopy and Spectroscopy

用扫描隧穿显微镜和光谱法探讨界面水的结构和动力学

Water/solid interfaces are ubiquitous and play a key role in many environmental, biophysical, and technological processes. Resolving the internal ...

Synthesis and Structure Determination of &#181;-Conotoxin PIIIA Isomers with Different Disulfide Connectivities

Synthesis and Structure Determination of ÃƒÆ’Ã¢â‚¬Å¡Ãƒâ€šÃ‚Âµ-Conotoxin PIIIA Isomers with Different Disulfide Connectivities

Peptides with a high number of cysteines are usually influenced regarding the three-dimensional structure by their disulfide connectivity. It is thus ...

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

利用毛细管区电泳串联质谱技术进行大尺度自上而下蛋白质组学

Capillary zone electrophoresis-electrospray ionization-tandem mass spectrometry (CZE-ESI-MS/MS) has been recognized as a useful tool for top-down ...

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

New analytical methods are urgently needed to enable high-throughput, yet comprehensive drug screening, given an alarming opioid and prescription drug ...