Name: skeletal muscle neurovascular coupling, oxidative capacity, and microvascular function with 'one stop shop' near-infrared spectroscopy
Uploaded: 2018-02-20T15:00:00
Description: Watch this Scientific Journal Video about Skeletal Muscle Neurovascular Coupling, Oxidative Capacity, and Microvascular Function with 'One Stop Shop' Near-infrared Spectroscopy at JoVE.com

この作品はテキサス大学アーリントン学際的研究助成校によって支えられました。

このプロトコルは次のテキサス大学アーリントン校で倫理委員会のガイドラインとヘルシンキ宣言の最新バージョンによって設定された標準に準拠しています。したがって、書面によるインフォームド コンセント (されする必要があります) の調査手続の開始前に取得しました。
1. 計装
注: 以下の計装説明、近赤外線 (NIR) 分光器とデータ集録システム...

<ol>
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C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Guidelines+for+the+ultrasound+assessment+of+endothelial-dependent+flow-mediated+vasodilation+of+the+brachial+artery+-+A+report+of+the+International+Brachial+Artery+Reactivity+Task+Force.">Guidelines for the ultrasound assessment of endothelial-dependent flow-mediated vasodilation of the brachial artery - A report of the International Brachial Artery Reactivity Task Force.</a> J Am Coll Cardiol. 39 (2), 257-265 (2002).</li><li>Thijssen, D. H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Assessment+of+flow-mediated+dilation+in+humans:+a+methodological+and+physiological+guideline.">Assessment of flow-mediated dilation in humans: a methodological and physiological guideline.</a> Am J Physiol Heart Circ Physiol. 300 (1), H2-H12 (2011).</li><li>Green, D. J., Jones, H., Thijssen, D., Cable, N. T., Atkinson, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Flow-mediated+dilation+and+cardiovascular+event+prediction:+does+nitric+oxide+matter?.">Flow-mediated dilation and cardiovascular event prediction: does nitric oxide matter?.</a> Hypertension. 57 (3), 363-369 (2011).</li><li>Southern, W. M., Ryan, T. E., Reynolds, M. A., McCully, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reproducibility+of+near-infrared+spectroscopy+measurements+of+oxidative+function+and+postexercise+recovery+kinetics+in+the+medial+gastrocnemius+muscle.">Reproducibility of near-infrared spectroscopy measurements of oxidative function and postexercise recovery kinetics in the medial gastrocnemius muscle.</a> Appl Physiol Nutr Metab. 39 (5), 521-529 (2014).</li><li>Ryan, T. E., Erickson, M. L., Brizendine, J. T., Young, H. J., McCully, K. 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L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comparison+of+four+different+vascular+occlusion+tests+for+assessing+reactive+hyperemia+using+near-infrared+spectroscopy.">Comparison of four different vascular occlusion tests for assessing reactive hyperemia using near-infrared spectroscopy.</a> Crit Care Med. 39 (4), 695-701 (2011).</li><li>McLay, K. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Vascular+responsiveness+determined+by+near-infrared+spectroscopy+measures+of+oxygen+saturation.">Vascular responsiveness determined by near-infrared spectroscopy measures of oxygen saturation.</a> Exp Physiol. 101 (1), 34-40 (2016).</li><li>McLay, K. M., Nederveen, J. P., Pogliaghi, S., Paterson, D. H., Murias, J. 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M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reduced+skeletal+muscle+oxidative+capacity+and+impaired+training+adaptations+in+heart+failure.">Reduced skeletal muscle oxidative capacity and impaired training adaptations in heart failure.</a> Physiol Rep. 3 (4), (2015).</li><li>Ryan, T. E., Brizendine, J. T., McCully, K. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+comparison+of+exercise+type+and+intensity+on+the+noninvasive+assessment+of+skeletal+muscle+mitochondrial+function+using+near-infrared+spectroscopy.">A comparison of exercise type and intensity on the noninvasive assessment of skeletal muscle mitochondrial function using near-infrared spectroscopy.</a> J Appl Physiol. 114 (2), 230-237 (2013).</li><li>Adami, A., Cao, R., Porszasz, J., Casaburi, R., Rossiter, H. 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記載方法は、1 回のクリニックや研究室訪問の反応性充血、血管カップリングおよび骨格筋有酸素能の非侵襲的、臨床評価を有効にします。
重要な考慮事項
これらのデータのコレクションは、腹部の筋肉の上に直接バイオケミカルの配置は動きの人工物を避けるために場所にしっかりと固定を避けるために薄暗い部屋で黒のビニ?...

組織虚血の短い期間に充血している応答 (マイクロ) 血管機能の重要な非侵襲的指標として浮上しています。導管動脈の閉塞、中に虚血性の侮辱を相殺するための努力で下流の細動脈を拡張させます。咬合のリリース時に血管抵抗の低下結果充血、大きさの下流の血管を拡張する能力によって決定されます。反応性充血は心血管イベント1,2したがって臨床的に重要なエンドポイントの強力な独立した予測が、許容範囲と生活の質を行使するその機能的意義は不明瞭です。
確かに、動的な運動は代謝需要への酸素供給を一致させるために非常に調整された血管応答を要求する主要な心血管ストレスを表します。たとえば、骨格筋血流は、孤立した筋収縮3、このような血行動態反応が全身運動に外挿する場合、心臓のポンプの能力を圧倒するだろう時にほぼ 100 倍増やすことができます。したがって、重度の低血圧、交感神経 (すなわち、血管収縮薬) を避けるために神経活動増加心臓出力非アクティブと内臓の組織から、アクティブな骨格筋4の方を再配布します。交感神経は運動筋の5; 監督もただし、適切な組織酸素配信6,7,8,9,10、を確保するために収縮反応を減衰させるローカル代謝シグナル11. このプロセスをまとめて機能 sympatholysis12と呼ばれる、運動時に骨格筋血流の正常な規則ことが不可欠です。骨格筋の血流は有酸素能力の重要な決定要因-生活の質と心血管疾患の罹患率と死亡率のための13の独立した予測因子-骨格筋血流と組織酸素の制御について運動中の配信は、臨床的意義は大きいです。
酸素供給は酸素利用率の方程式の残りの半分を満たす Fick の方程式の半分だけをただし、です。中での酸素利用率の主要な規定、ミトコンドリアの酸化的リン酸化は、安静時と運動時の細胞プロセスの十分なエネルギーの供給に重要な役割を果たしています。確かに、筋有酸素能の障害は、機能的な能力と生活の質14,15,16を制限できます。様々 な対策は、侵襲的な筋肉の生検と高価で時間のかかる磁気共鳴分光学 (夫人) の技術を含む筋有酸素能のインデックスを提供するために使用されます。
ここでは、我々 単一診療所や研究室訪問で各 3 つ主要な臨床的エンドポイント (反応性充血、sympatholysis、および筋有酸素能) を評価するために近赤外分光法 (NIRS) を用いた新規、非侵襲的アプローチを提案します。このアプローチの主要な利点が 3 つあります: 最初に、この手法は、持ち運びも簡単、比較的低コスト、簡単に実行できます。反応性充血を測定するための現在のドップラー超音波方法はオペレーターに強く依存して-トレーニングと広範なスキルを必要とする-、高コスト、高度なデータ集録ハードウェアおよび後処理ソフトウェアを必要とします。さらに、これは多分クリニックおよび/またはベッドサイド監視または治療効果をテストの大規模な臨床試験に導入する。第二に、方法論のおかげでこの手法の具体的手法の全体的な特異性増加、骨格筋の微小血管に焦点を当てます。超音波ドプラを用いた代替的なアプローチは上流管容器に完全に集中し、下流の信号を抑制することができます変更を推測します。第三に、この手法は、完全に非侵襲的です。骨格筋の酸化能力は伝統的侵襲の評価し、痛みを伴う筋肉の生検と機能 sympatholysis の交感神経と sympatholytics の動脈内注入と評価があります。この方法では、これらの要件をすべて一緒に回避できます。

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			<td style="width:167px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:328px;">NIRS muscle sensor</td>
			<td style="width:223px;">Iss Medical</td>
			<td align="right" style="width:119px;">201.2</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:328px;">E20 Rapid cuff inflation system</td>
			<td style="width:223px;">Hokanson</td>
			<td style="width:119px;">E20</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:328px;">AG101 Air Source</td>
			<td style="width:223px;">Hokanson</td>
			<td style="width:119px;">AG101</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:328px;">Smedley Handgrip dynometer (recording)</td>
			<td style="width:223px;">Stolting</td>
			<td align="right" style="width:119px;">56380</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:328px;">Powerlab 16/35, 16 Channel Recorder</td>
			<td style="width:223px;">ADInstruments</td>
			<td style="width:119px;">PL3516</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:328px;">Human NIBP Set</td>
			<td style="width:223px;">ADInstruments</td>
			<td style="width:119px;">ML282-SM</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:328px;">Bio Amp</td>
			<td style="width:223px;">ADInstruments</td>
			<td style="width:119px;">FE132</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:328px;">Quad Bridge Amp</td>
			<td style="width:223px;">ADInstruments</td>
			<td style="width:119px;">FE224</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:328px;">Connex Spot Monitor</td>
			<td style="width:223px;">Welch Allyn</td>
			<td style="width:119px;">71WX-B</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:328px;">Origin(Pro) graphing software</td>
			<td style="width:223px;">OrignPro</td>
			<td style="width:119px;">Pro</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:328px;">Lower body negative pressure chamber</td>
			<td style="width:223px;">Physiology Research Instruments</td>
			<td style="width:119px;">standard unit</td>
			<td></td>
		</tr>
	</tbody>
</table>

skeletal muscle neurovascular coupling, oxidative capacity, and microvascular function with 'one stop shop' near-infrared spectroscopy

運動は代謝需要への酸素供給を一致させるために非常に調整された血管応答を要求する主要な血行力学的ストレスを表します。(組織虚血の短い期間への応答) の反応性充血心血管イベントの独立した予測因子血管健康状態および血管拡張能の重要な洞察を提供しています。骨格筋の酸化能力は、myocellular プロセスのためのエネルギー供給を決定、健康と病気も重要です。ここでは、単一のクリニックや研究室訪問の間に各主要な臨床的エンドポイント (反応性充血、血管カップリングと筋有酸素能) を評価するために近赤外分光法を用いた単純な非侵襲的なアプローチについて述べる。ドップラー超音波、磁気共鳴画像/分光法、または侵襲的なカテーテル ベースのフロー測定または筋肉の生検とは異なり我々 のアプローチは、少ないオペレーター依存、低コスト、完全に非侵襲的です。以前公表された資料から要約データと一緒に研究室から代表的なデータでは、これらのエンドポイントのそれぞれのユーティリティについて説明します。一度このテクニックをマスターすると、個体群の臨床への応用は運動不耐症、心血管障害の重要な機械論的洞察を提供します。

骨格筋の酸化能力
図 2は、放医研から派生した骨格筋の酸化能力評価中に代表的な参加者の応答を示します。パネル A ショー組織飽和度プロファイル動脈 5 分間カフ オクルー ジョン プロトコル、ハンド グリップ運動、運動からの回復の間に断続的な動脈閉塞。パネル B は、回復...

Watch this Scientific Journal Video about Skeletal Muscle Neurovascular Coupling, Oxidative Capacity, and Microvascular Function with 'One Stop Shop' Near-infrared Spectroscopy at JoVE.com

Skeletal Muscle Neurovascular Coupling, Oxidative Capacity, and Microvascular Function with 'One Stop Shop' Near-infrared Spectroscopy

骨格筋血管カップリング、酸化容量、および 'ワンストップ ショップ「近赤外分光法を用いた微小血管機能

ここでは、単一のクリニックや研究室訪問の反応性充血、脳循環代謝、骨格筋の酸化能力を評価するために近赤外分光法を用いた単純な非侵襲的なアプローチについて述べる。

Exercise represents a major hemodynamic stress that demands a highly coordinated neurovascular response in order to match oxygen delivery to metabolic ...

Research

JoVE Journal

Medicine

Myo-mechanical Analysis of Isolated Skeletal Muscle

単離骨格筋のミオ - 機械的分析

To assess the in vivo effects of therapeutic interventions for the treatment of muscle disease 1,2,3, quantitative methods are needed that measure force ...

In Vivo Canine Muscle Function Assay

In Vivo Canine Muscle Function Assay

ビボ犬筋肉の機能アッセイに

We describe a minimally-invasive and reproducible method to measure canine pelvic limb muscle strength and muscle response to repeated eccentric 
...

Skeletal Muscle Gender Dimorphism from Proteomics

プロテオミクスから骨格筋の性別二型

Gross contraction in skeletal muscle is primarily determined by a relatively small number of contractile proteins, however this tissue is also remarkably ...

Non-invasive Assessment of Microvascular and Endothelial Function

微小血管と内皮機能の非侵襲的評価

The authors have utilized capillaroscopy and forearm blood flow techniques to investigate the role of microvascular dysfunction in pathogenesis of ...

Quantitative Magnetic Resonance Imaging of Skeletal Muscle Disease

骨格筋疾患の定量的磁気共鳴イメージング

Quantitative magnetic resonance imaging (qMRI) describes the development and use of MRI to quantify physical, chemical, and/or biological properties of ...

Combined Near-infrared Fluorescent Imaging and Micro-computed Tomography for Directly Visualizing Cerebral Thromboemboli

直接脳血栓塞栓を可視化するための複合近赤外蛍光イメージングおよびマイクロコンピュータ断層撮影

Direct thrombus imaging visualizes the root cause of thromboembolic infarction. Being able to image thrombus directly allows far better investigation of ...

Induction and Assessment of Exertional Skeletal Muscle Damage in Humans

ヒトにおける労作骨格筋損傷の誘導および評価

Contraction-induced muscle damage via voluntary eccentric (lengthening) contractions offers an excellent model for studying muscle adaptation and recovery ...

Phosphorus-31 Magnetic Resonance Spectroscopy: A Tool for Measuring In Vivo Mitochondrial Oxidative Phosphorylation Capacity in Human Skeletal Muscle

Phosphorus-31 Magnetic Resonance Spectroscopy: A Tool for Measuring In Vivo Mitochondrial Oxidative Phosphorylation Capacity in Human Skeletal Muscle

リン-31磁気共鳴分光法：測定するためのツール<em&gt;インビボ</emヒト骨格筋における&gt;ミトコンドリア酸化的リン酸化能力

Skeletal muscle mitochondrial oxidative phosphorylation (OXPHOS) capacity, which is critically important in health and disease, can be measured in vivo ...

Assessment of Human Adipose Tissue Microvascular Function Using Videomicroscopy

顕微鏡を用いたひと脂肪組織血管機能評価

While obesity is closely linked to the development of metabolic and cardiovascular disease, little is known about mechanisms that govern these processes. ...

A New Best Practice for Validating Tail Vein Injections in Rat with Near-infrared-Labeled Agents

近く-赤外線-ラベル エージェントとラットの尾静脈注射を検証するための新しいベスト プラクティス

Intravenous (IV) administration of agents into the tail vein of rats can be both difficult and inconsistent. Optimizing tail vein injections is a key part ...

骨格筋血管カップリング、酸化容量、および 'ワンストップ ショップ「近赤外分光法を用いた微小血管機能

骨格筋血管カップリング、酸化容量、および 'ワンストップショップ「近赤外分光法を用いた微小血管機能