Name: dual effects of melanoma cell-derived factors on bone marrow adipocytes differentiation
Uploaded: 2018-08-23T12:30:00
Description: Watch this Scientific Journal Video about Dual Effects of Melanoma Cell-derived Factors on Bone Marrow Adipocytes Differentiation at JoVE.com

Wir danken Dov Zipori (Weizmann Institute of Science, Rehovot, Israel) freundlicherweise für das Bereitstellen der murinen Knochenmark Stromazellen Zelle Linie 14F1.1. Diese Studie wurde unterstützt durch Zuschüsse von der chinesischen National Natural Science Foundation (Nr. 81771729) und der Tropical Hospital von Chongqing Medical University (Nos. YJQN201330; YJZQN201527).

Hinweis: Alle Zellen, die in diesem Protokoll verwendeten sollte für mindestens drei Generationen nach dem Auftauen von gefrorenem Lager Zellen angebaut werden.
1. Ernte Melanom Zelle abgeleitet Faktoren
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Vorbereitungen
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<li>Erhalten Sie B16F10 Zellen und eine Maus-Melanom-Zell-Linie. Hinweis: Für dieses Protokoll war eine Maus Melanom-Zelllinie Stammzellenbank der chinesischen Akademie der Wissenschaften entnommen.</li>
		<li>Machen Sie einen kompletten Me...

<ol>
	<li>Wang, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Adipogenic+niches+for+melanoma+cell+colonization+and+growth+in+bone+marrow.">Adipogenic niches for melanoma cell colonization and growth in bone marrow.</a> Laboratory Investigation. 97 (6), 737-745 (2017).</li><li>Trotter, T. N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Adipocyte-Lineage+Cells+Support+Growth+and+Dissemination+of+Multiple+Myeloma+in+Bone.">Adipocyte-Lineage Cells Support Growth and Dissemination of Multiple Myeloma in Bone.</a> The American Journal of Pathology. 186 (11), 3054-3063 (2016).</li><li>Morris, E. V., Edwards, C. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+role+of+bone+marrow+adipocytes+in+bone+metastasis.">The role of bone marrow adipocytes in bone metastasis.</a> Journal of Bone Oncology. 5 (3), 121-123 (2016).</li><li>Diedrich, J. D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Bone+marrow+adipocytes+promote+the+Warburg+phenotype+in+metastatic+prostate+tumors+via+HIF-1alpha+activation.">Bone marrow adipocytes promote the Warburg phenotype in metastatic prostate tumors via HIF-1alpha activation.</a> Oncotarget. 7 (40), 64854-64877 (2016).</li><li>Chkourko Gusky, H., Diedrich, J., MacDougald, O. A., Podgorski, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Omentum+and+bone+marrow:+how+adipocyte-rich+organs+create+tumour+microenvironments+conducive+for+metastatic+progression.">Omentum and bone marrow: how adipocyte-rich organs create tumour microenvironments conducive for metastatic progression.</a> Obesity Reviews. 17 (11), 1015-1029 (2016).</li><li>Chen, G. L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=High+fat+diet+increases+melanoma+cell+growth+in+the+bone+marrow+by+inducing+osteopontin+and+interleukin+6.">High fat diet increases melanoma cell growth in the bone marrow by inducing osteopontin and interleukin 6.</a> Oncotarget. 7 (18), 26653-26669 (2016).</li><li>Balaban, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Adipocyte+lipolysis+links+obesity+to+breast+cancer+growth:+adipocyte-derived+fatty+acids+drive+breast+cancer+cell+proliferation+and+migration.">Adipocyte lipolysis links obesity to breast cancer growth: adipocyte-derived fatty acids drive breast cancer cell proliferation and migration.</a> Cancer &amp; Metabolism. 5, 1 (2017).</li><li>Huang, C. K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Adipocytes+promote+malignant+growth+of+breast+tumours+with+monocarboxylate+transporter+2+expression+via+beta-hydroxybutyrate.">Adipocytes promote malignant growth of breast tumours with monocarboxylate transporter 2 expression via beta-hydroxybutyrate.</a> Nature Communications. 8, 14706 (2017).</li><li>Wang, Y. Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mammary+adipocytes+stimulate+breast+cancer+invasion+through+metabolic+remodeling+of+tumor+cells.">Mammary adipocytes stimulate breast cancer invasion through metabolic remodeling of tumor cells.</a> JCI Insight. 2 (4), 87489 (2017).</li><li>Wang, C., Gao, C., Meng, K., Qiao, H., Wang, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Human+adipocytes+stimulate+invasion+of+breast+cancer+MCF-7+cells+by+secreting+IGFBP-2.">Human adipocytes stimulate invasion of breast cancer MCF-7 cells by secreting IGFBP-2.</a> PLoS One. 10 (3), 0119348 (2015).</li><li>Nieman, K. 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S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Breast+Cancer+Cell+Colonization+of+the+Human+Bone+Marrow+Adipose+Tissue+Niche.">Breast Cancer Cell Colonization of the Human Bone Marrow Adipose Tissue Niche.</a> Neoplasia. 17 (12), 849-861 (2015).</li><li>Daquinag, A. C., Souza, G. R., Kolonin, M. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Adipose+tissue+engineering+in+three-dimensional+levitation+tissue+culture+system+based+on+magnetic+nanoparticles.">Adipose tissue engineering in three-dimensional levitation tissue culture system based on magnetic nanoparticles.</a> Tissue Engineering Part C: Methods. 19 (5), 336-344 (2013).</li><li>Emont, M. 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F., Yang, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Three-dimensional+cell+culture+systems+and+their+applications+in+drug+discovery+and+cell-based+biosensors.">Three-dimensional cell culture systems and their applications in drug discovery and cell-based biosensors.</a> ASSAY and Drug Development Technologies. 12 (4), 207-218 (2014).</li><li>Herroon, M. K., Diedrich, J. D., Podgorski, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=New+3D-Culture+Approaches+to+Study+Interactions+of+Bone+Marrow+Adipocytes+with+Metastatic+Prostate+Cancer+Cells.">New 3D-Culture Approaches to Study Interactions of Bone Marrow Adipocytes with Metastatic Prostate Cancer Cells.</a> Frontiers in Endocrinology (Lausanne). 7, 84 (2016).</li><li>Lee, J. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+three-dimensional+microenvironment+alters+protein+expression+and+chemosensitivity+of+epithelial+ovarian+cancer+cells+in+vitro.">A three-dimensional microenvironment alters protein expression and chemosensitivity of epithelial ovarian cancer cells in vitro.</a> Laboratory Investigation. 93 (5), 528-542 (2013).</li><li>Imamura, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comparison+of+2D-+and+3D-culture+models+as+drug-testing+platforms+in+breast+cancer.">Comparison of 2D- and 3D-culture models as drug-testing platforms in breast cancer.</a> Oncology Reports. 33 (4), 1837-1843 (2015).</li><li>Birgersdotter, A., Sandberg, R., Ernberg, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Gene+expression+perturbation+in+vitro--a+growing+case+for+three-dimensional+(3D)+culture+systems.">Gene expression perturbation in vitro--a growing case for three-dimensional (3D) culture systems.</a> Seminars in Cancer Biology. 15 (5), 405-412 (2005).</li><li>Wang, W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=3D+spheroid+culture+system+on+micropatterned+substrates+for+improved+differentiation+efficiency+of+multipotent+mesenchymal+stem+cells.">3D spheroid culture system on micropatterned substrates for improved differentiation efficiency of multipotent mesenchymal stem cells.</a> Biomaterials. 30 (14), 2705-2715 (2009).</li><li>Dirat, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cancer-associated+adipocytes+exhibit+an+activated+phenotype+and+contribute+to+breast+cancer+invasion.">Cancer-associated adipocytes exhibit an activated phenotype and contribute to breast cancer invasion.</a> Cancer Research. 71 (7), 2455-2465 (2011).</li><li>Scott, M. A., Nguyen, V. T., Levi, B., James, A. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Current+methods+of+adipogenic+differentiation+of+mesenchymal+stem+cells.">Current methods of adipogenic differentiation of mesenchymal stem cells.</a> Stem Cells and Development. 20 (10), 1793-1804 (2011).</li><li>Zipori, D., Toledo, J., von der Mark, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Phenotypic+heterogeneity+among+stromal+cell+lines+from+mouse+bone+marrow+disclosed+in+their+extracellular+matrix+composition+and+interactions+with+normal+and+leukemic+cells.">Phenotypic heterogeneity among stromal cell lines from mouse bone marrow disclosed in their extracellular matrix composition and interactions with normal and leukemic cells.</a> Blood. 66 (2), 447-455 (1985).</li><li>Maridas, D. 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(132), e56850 (2018).</li><li>Bozec, A., Hannemann, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mechanism+of+Regulation+of+Adipocyte+Numbers+in+Adult+Organisms+Through+Differentiation+and+Apoptosis+Homeostasis.">Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis.</a> Journal of Visualized Experiments. (112), e53822 (2016).</li><li>Shafat, M. 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Kokulturen mit Einsätzen wurden weithin zur Zell-Zell-Interaktionen zu untersuchen. Das 2D Coculture System ist eine effektive Möglichkeit zu beobachten, wie die beiden Teile durch die zeigten wir hier zwei verschiedene Krebs Zelle-angetrieben Auswirkungen auf Knochenmark Adipozyten, Übersprechen in Vitro. Viele Labore haben diese Methode, um das Übersprechen zwischen Adipozyten und Krebs Zellen6,12,27,<s...

Knochenmetastasen sind weit verbreitet bei fortgeschrittenem Krebs-Patienten, aber eine kurative Behandlung ist noch nicht verfügbar. Darüber hinaus spezialisiert auf Energie als Fett zu speichern, unterstützen Adipozyten Tumorwachstum und Metastasierung im Knochenmark und anderen Organen1,2,3,4,5,6. Darüber hinaus spielen Adipozyten eine wesentliche Rolle bei der Regulierung der Krebs Zelle Biologie7,8,9,10 und Stoffwechsel4,11,12 ,13,14,15,16, so gut wie in Knochen Metastasen1,4,12. In der Nische des Knochenmarks können Adipozyten auch Auswirkungen auf das biologische Verhalten von Krebs Zellen4,6,17. Das Zusammenspiel von Knochenmark Adipozyten und Krebszellen mit Osteotropism ist bedeutsam für das Verständnis von Knochenmetastasen. Jedoch ist wenig bekannt.
Basierend auf aktuellen Studien, werden verschiedene Methoden auf Adipozyten, einschließlich zwei- oder dreidimensionale (2/3D) und ex-Vivo Kulturen17,18,19,20,21angewendet. Vor kurzem, Herroon Et Al. entwickelt ein neues 3D-Kultur-Konzept für Interaktionen von Knochenmark Adipozyten mit Krebs Zellen22zu untersuchen. Obwohl die 3D Coculture ist optimal für die Nachahmung physiologischen Wechselwirkungen zwischen Adipozyten und Krebs-Zellen in Vivo, es leidet unter schlechten Reproduzierbarkeit22,23. Im Vergleich zu einem 2D Coculture System kann ein 3D Coculture System verschiedene zelluläre Phänotypen, wie Zelle Morphologie21,22,24,25,26vorsehen. Darüber hinaus kann die ex-Vivo -Kultur der isolierten Spongiosa gewebefragmente zu einer robusten Auswuchs der Adipozyten aus kultivierten Knochenmark Zellen17führen.
Im Gegensatz zu diesen früheren Modellen bleibt jedoch das 2D zellenmodell Kultur eine klassisch, zuverlässige und einfache Technik für schnell scannen Kandidat Moleküle und die Phänotypen verändert in Adipozyten oder Krebs in Vitro1Zellen, 4,6,12,15,27. Um das Übersprechen zwischen dem Knochenmark Adipozyten und Melanomzellen besser verstehen zu können, bieten wir ein detailliertes Protokoll für ein 2D Coculture Knochenmark Adipozyten mit Melanomzellen.

<table border="0" cellpadding="0" cellspacing="0" style="width:684px;" width="685">
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		<col />
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	</colgroup>
	<tbody>
		<tr height="17">
			<td height="17" style="height:18px;width:215px;">DMEM</td>
			<td style="width:140px;">Invitrogen Inc.</td>
			<td style="width:143px;">11965092</td>
			<td style="width:187px;"></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:215px;">Fetal Bovine Serum</td>
			<td style="width:140px;">Invitrogen Inc.</td>
			<td style="width:143px;">16000&#8211;044</td>
			<td style="width:187px;"></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:215px;">Phosphate Buffered Saline</td>
			<td style="width:140px;">Invitrogen Inc.</td>
			<td style="width:143px;">14190-144</td>
			<td style="width:187px;"></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:215px;">Insulin</td>
			<td style="width:140px;">Sigma-Aldrich</td>
			<td style="width:143px;">91077C</td>
			<td style="width:187px;"></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:215px;">3-isobutyl-1-methyl-xanthine</td>
			<td style="width:140px;">Sigma-Aldrich</td>
			<td style="width:143px;">I5879</td>
			<td style="width:187px;"></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:215px;">Dexamethasone</td>
			<td style="width:140px;">Sigma-Aldrich</td>
			<td style="width:143px;">D4902</td>
			<td style="width:187px;"></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:215px;">Oil Red o</td>
			<td style="width:140px;">Sigma-Aldrich</td>
			<td style="width:143px;">O0625</td>
			<td style="width:187px;"></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:215px;">24-well plate</td>
			<td style="width:140px;">Corning</td>
			<td style="width:143px;">CLS3527</td>
			<td style="width:187px;"></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:215px;">Transwell insert</td>
			<td style="width:140px;">Millipore</td>
			<td style="width:143px;">MCHT24H48</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:215px;">Penicillin/Streptomycin</td>
			<td style="width:140px;">Invitrogen</td>
			<td style="width:143px;">15140-122</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">isopropanol</td>
			<td style="width:140px;">Sigma-Aldrich</td>
			<td style="width:143px;">I9516</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:215px;">0.25% trypsin</td>
			<td style="width:140px;">Thermo Scientific</td>
			<td style="width:143px;">25200056</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:215px;">hemocytometer</td>
			<td style="width:140px;">Bio-Rad</td>
			<td style="width:143px;">1450016</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:215px;">Culture incubator</td>
			<td style="width:140px;">Thermo Scientific</td>
			<td style="width:143px;"></td>
			<td style="width:187px;"></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:215px;">50ml falcon</td>
			<td style="width:140px;">Corning</td>
			<td style="width:143px;">CLS430828</td>
			<td style="width:187px;"></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:215px;">Clean Bench</td>
			<td style="width:140px;">Thermo Scientific</td>
			<td style="width:143px;">-</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:215px;">Microscopy</td>
			<td style="width:140px;">Olympus</td>
			<td style="width:143px;">-</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:215px;">200 &#956;L pipet tips</td>
			<td style="width:140px;">BeyoGold</td>
			<td style="width:143px;">FTIP620</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:215px;">1000 mL pipet tips</td>
			<td style="width:140px;">BeyoGold</td>
			<td style="width:143px;">FTIP628</td>
			<td></td>
		</tr>
	</tbody>
</table>

dual effects of melanoma cell-derived factors on bone marrow adipocytes differentiation

Das Übersprechen zwischen dem Knochenmark Adipozyten und Tumorzellen kann eine entscheidende Rolle im Prozess der Knochenmetastasen. Eine Vielzahl von Methoden stehen zur Verfügung für das bedeutende Übersprechen zu studieren; ein zweidimensionales Transwell-System für die Coculture bleibt jedoch ein Klassiker, zuverlässig, und einfache Möglichkeit für diese Studie Übersprechen. Hier präsentieren wir Ihnen ein detailliertes Protokoll, das die Coculture von Knochenmark Adipozyten und Melanomzellen zeigt. Dennoch könnte ein solches Coculture System nicht nur einen Beitrag zur Erforschung der Zelle Signal Transductions von Krebszellen durch Knochenmark Adipozyten induziert, sondern auch in die Zukunft mechanistischen Studie von Knochenmetastasen die neue therapeutische Zielstrukturen für Knochen zeigen kann Metastasierung.

Im Knochenmark, Adipozyten können in den Tumor Mikroumgebung1,13,33,34,35 in einem frühen Stadium zur Unterstützung der Tumorprogression durch lösliche Faktoren erscheinen oder Osteoclastogenesis6,12,36, vor allem im Zusammenhang m...

Watch this Scientific Journal Video about Dual Effects of Melanoma Cell-derived Factors on Bone Marrow Adipocytes Differentiation at JoVE.com

Dual Effects of Melanoma Cell-derived Factors on Bone Marrow Adipocytes Differentiation

Hier präsentieren wir Ihnen eine zuverlässige und einfache zweidimensionale (2D) Coculture-System für das Studium der Interaktion zwischen Tumorzellen und Knochenmark Adipozyten, die eine doppelte Wirkung von Melanom Zelle abgeleitet Faktoren auf das Knochenmark Adipozyten enthüllt Differenzierung und stellt auch eine klassische Methode für die mechanistischen Studie von Knochenmetastasen.

Dual Effekte von Melanom Zelle abgeleitet Faktoren auf Knochenmark Adipozyten Differenzierung

The crosstalk between bone marrow adipocytes and tumor cells may play a critical role in the process of bone metastasis. A variety of methods are ...

Research

JoVE Journal

Cancer Research

Conditional Knockdown of Gene Expression in Cancer Cell Lines to Study the Recruitment of Monocytes/Macrophages to the Tumor Microenvironment

Bedingte Knockdown von Genexpression in Krebs-Zell-Linien, die Rekrutierung von Monozyten/Makrophagen, den Tumor Mikroumgebung zu studieren

siRNA and shRNA-mediated knock down (KD) methods of regulating gene expression are invaluable tools for understanding gene and protein function. However, ...

Forschung

Krebsforschung

Comprehensive Protocol to Sample and Process Bone Marrow for Measuring Measurable Residual Disease and Leukemic Stem Cells in Acute Myeloid Leukemia

Umfassendes Protokoll zur Probe und Prozess des Knochenmarks zur Messung von messbaren Resterkrankung und leukämischen Stammzellen in der akuten myeloischen Leukämie

Response criteria in acute myeloid leukemia (AML) has recently been re-established, with morphologic examination utilized to determine whether patients ...

A 3D Organotypic Melanoma Spheroid Skin Model

Ein 3D organotypischen Melanom Sphäroid Hautmodell

Malignant transformation of melanocytes, the pigment cells of human skin, causes formation of melanoma, a highly aggressive cancer with increased ...

Multimodal Bioluminescent and Positronic-emission Tomography/Computational Tomography Imaging of Multiple Myeloma Bone Marrow Xenografts in NOG Mice

Multimodale Biolumineszenz und Positronic-Emissions-Tomographie/Computational Tomographie Bildgebung des multiplen Myeloms Knochenmark Xenotransplantate NOG Mäuse

Multiple myeloma (MM) tumors engraft in the bone marrow (BM) and their survival and progression are dependent upon complex molecular and cellular ...

A Melanoma Patient-Derived Xenograft Model

Ein melanompatientenabgeleitetes Xenograft-Modell

Accumulating evidence suggests that molecular and biological properties differ in melanoma cells grown in traditional two-dimensional tissue culture ...

Generation of a Liver Orthotopic Human Uveal Melanoma Xenograft Platform in Immunodeficient Mice

Generierung einer Leber orthotopischen Human Enuveal Melanom Xenograft Plattform bei immundefizienten Mäusen

In recent decades, subcutaneously implanted patient-derived xenograft tumors or cultured human cell lines have been increasingly recognized as more ...

Characterization of the Effects of Migrastatic Inhibitors on 3D Tumor Spheroid Invasion by High-resolution Confocal Microscopy

Charakterisierung der Auswirkungen von Migrastatischen Inhibitoren auf die 3D-Tumorsphäroid-Invasion durch hochauflösende Konfokalmikroskopie

Drug discovery and development in cancer research is increasingly being based on drug screens in a 3D format. Novel inhibitors targeting the migratory and ...

Modeling Primary Bone Tumors and Bone Metastasis with Solid Tumor Graft Implantation into Bone

Modellierung von primären Knochentumoren und Knochenmetastasen mit Implantation eines soliden Tumortransplantats in den Knochen

Primary bone tumors or bone metastasis from solid tumors result in painful osteolytic, osteoblastic, or mixed osteolytic/osteoblastic lesions. These ...

Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis

Kartierung der Struktur-Funktions-Beziehungen ungeordneter onkogener Transkriptionsfaktoren mittels Transkriptomanalyse

Many cancers are characterized by chromosomal translocations which result in the expression of oncogenic fusion transcription factors. Typically, these ...

Modeling the Effects of Hemodynamic Stress on Circulating Tumor Cells using a Syringe and Needle

Modellierung der Auswirkungen von hämodynamischem Stress auf zirkulierende Tumorzellen mit Spritze und Nadel

During metastasis, cancer cells from solid tissues, including epithelia, gain access to the lymphatic and hematogenous circulation where they are exposed ...