Mayor genoma edición con Cas9 ribonucleoproteína en diversas células y organismos
May 25th, 2018
•Behnom Farboud1,2*, Erin Jarvis1*, Theodore L. Roth3,4,5,6*, Jiyung Shin1,3*, Jacob E. Corn1,3, Alexander Marson3,5,6,7,8,9, Barbara J. Meyer1,2, Nipam H. Patel1,10, Megan L. Hochstrasser3
1Department of Molecular Cell Biology, University of California, Berkeley, 2Howard Hughes Medical Institute, University of California, Berkeley, 3Innovative Genomics Institute, University of California, Berkeley, 4Biomedical Sciences Graduate Program, University of California, San Francisco, 5Department of Microbiology and Immunology, University of California, San Francisco, 6Diabetes Center, University of California, San Francisco, 7Chan Zuckerberg Biohub, 8Department of Medicine, University of California, San Francisco, 9UCSF Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, 10Department of Integrative Biology, University of California, Berkeley
Utilizando un Cas9 Confecciones complejo de ribonucleoproteína (RNP) es un método eficaz para la edición del genoma exacto, eficiente. Aquí, podemos destacar su utilidad en una amplia gama de células y organismos, incluyendo las células humanas primarias y ambos clásicos y emergentes en organismos modelo.
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