Name: assessment of plasma coagulation on liver tissue in a large animal model in vivo
Uploaded: 2018-08-04T13:00:00
Description: Watch this Scientific Journal Video about Assessment of Plasma Coagulation on Liver Tissue in a Large Animal Model In Vivo at JoVE.com

Forfatterne har ingen takk.

Regler underlagt tysk lovgivning for dyrestudier samt prinsipper av laboratoriet Animal Care (National Institutes of Health publikasjonen ed. 8, 2011) ble fulgt. Offisiell tillatelse fra statlige dyr omsorg office (Landesamt für Natur, Umwelt und Verbraucherschutz Nordrhein-Westfalen, Recklinghausen, Tyskland).
1. dyr
<ol><li>Bruk kvinnelige tyske landrace griser (på 25-30 kg) i åpne burene.</li>
	<li>Bruk 5 dyr per gruppe (argon og helium).</li>
	<li>Tillat dyr til acclimatize omgivelsen...

<ol>
	<li>Link, W. J., Incropera, F. P., Glover, J. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+plasma+scalpel:+comparison+of+tissue+damage+and+wound+healing+with+electrosurgical+and+steel+scalpels.">A plasma scalpel: comparison of tissue damage and wound healing with electrosurgical and steel scalpels.</a> ArchSurg. 111, 392-397 (1976).</li><li>Kwon, A. H., Inui, H., Kamiyama, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Successful+laparoscopic+haemostasis+using+an+argon+beam+coagulator+for+blunt+traumatic+splenic+injury.">Successful laparoscopic haemostasis using an argon beam coagulator for blunt traumatic splenic injury.</a> EurJSurg. 167, 316-318 (2001).</li><li>Frilling, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effectiveness+of+a+new+carrier-bound+fibrin+sealant+versus+argon+beamer+as+haemostatic+agent+during+liver+resection:+a+randomised+prospective+trial.">Effectiveness of a new carrier-bound fibrin sealant versus argon beamer as haemostatic agent during liver resection: a randomised prospective trial.</a> Langenbecks ArchSurg. 390, 114-120 (2005).</li><li>Raiser, J., Zenker, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Argon+plasma+coagulation+for+open+surgical+and+endoscopic+applications:+state+of+the+art.">Argon plasma coagulation for open surgical and endoscopic applications: state of the art.</a> J Phys Appl Phys. 39 (16), 3520-3523 (2006).</li><li>Farin, G., Grund, K. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Technology+of+argon+plasma+coagulation+with+particular+regard+to+endoscopic+applications.">Technology of argon plasma coagulation with particular regard to endoscopic applications.</a> EndoscSurgAllied Technol. 2, 71-77 (1994).</li><li>Grund, K. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Argon+plasma+coagulation+(APC):+ballyhoo+or+breakthrough?.">Argon plasma coagulation (APC): ballyhoo or breakthrough?.</a> Endoscopy. 29, 196-198 (1997).</li><li>Glowka, T. R., Standop, J., Paschenda, P., Czaplik, M., Kalff, J. C., Tolba, R. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Argon+and+helium+plasma+coagulation+of+porcine+liver+tissue.">Argon and helium plasma coagulation of porcine liver tissue.</a> J Int Med Res. , (2017).</li><li>Dowling, R. D., Ochoa, J., Yousem, S. A., Peitzman, A., Udekwu, A. O. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Argon+beam+coagulation+is+superior+to+conventional+techniques+in+repair+of+experimental+splenic+injury.">Argon beam coagulation is superior to conventional techniques in repair of experimental splenic injury.</a> JTrauma. 31, 717-720 (1991).</li><li>Go, P. M., Goodman, G. R., Bruhn, E. W., Hunter, J. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+argon+beam+coagulator+provides+rapid+hemostasis+of+experimental+hepatic+and+splenic+hemorrhage+in+anticoagulated+dogs.">The argon beam coagulator provides rapid hemostasis of experimental hepatic and splenic hemorrhage in anticoagulated dogs.</a> JTrauma. 31, 1294-1300 (1991).</li><li>Brand, C. U., Blum, A., Schlegel, A., Farin, G., Garbe, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Application+of+argon+plasma+coagulation+in+skin+surgery.">Application of argon plasma coagulation in skin surgery.</a> Dermatology. 197, 152-157 (1998).</li><li>Carus, T., Rackebrandt, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Collateral+tissue+damage+by+several+types+of+coagulation+(monopolar,+bipolar,+cold+plasma+and+ultrasonic)+in+a+minimally+invasive,+perfused+liver+model.">Collateral tissue damage by several types of coagulation (monopolar, bipolar, cold plasma and ultrasonic) in a minimally invasive, perfused liver model.</a> ISRNSurg. , 518924 (2011).</li><li>Bludau, M., Vallbohmer, D., Gutschow, C., Holscher, A. H., Schroder, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Quantitative+measurement+of+gastric+mucosal+microcirculation+using+a+combined+laser+Doppler+flowmeter+and+spectrophotometer.">Quantitative measurement of gastric mucosal microcirculation using a combined laser Doppler flowmeter and spectrophotometer.</a> DisEsophagus. , (2008).</li><li>Beckert, S., Witte, M. B., Konigsrainer, A., Coerper, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+impact+of+the+Micro-Lightguide+O2C+for+the+quantification+of+tissue+ischemia+in+diabetic+foot+ulcers.">The impact of the Micro-Lightguide O2C for the quantification of tissue ischemia in diabetic foot ulcers.</a> Diabetes Care. 27, 2863-2867 (2004).</li><li>Erdogan, D., de Graaf, W., van Gulik, T. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Adhesive+strength+of+fibrinogen-coated+collagen+patch+or+liquid+fibrin+sealant+in+an+experimental+liver+resection+model+in+pigs.">Adhesive strength of fibrinogen-coated collagen patch or liquid fibrin sealant in an experimental liver resection model in pigs.</a> Eur Surg Res Eur Chir Forsch Rech Chir Eur. 41 (3), 298-302 (2008).</li><li>Knobloch, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Microcirculation+of+the+sternum+following+harvesting+of+the+left+internal+mammary+artery.">Microcirculation of the sternum following harvesting of the left internal mammary artery.</a> ThoracCardiovascSurg. 51, 255-259 (2003).</li><li>Kanzler, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Recommendation+for+severity+assessment+following+liver+resection+and+liver+transplantation+in+rats:+Part+I.">Recommendation for severity assessment following liver resection and liver transplantation in rats: Part I.</a> Lab Anim. 50 (6), 459-467 (2016).</li><li>Pehb&#246;ck, D., Dietrich, H., Klima, G., Paal, P., Lindner, K. H., Wenzel, V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Anesthesia+in+swine+optimizing+a+laboratory+model+to+optimize+translational+research.">Anesthesia in swine optimizing a laboratory model to optimize translational research.</a> Anaesthesist. 64 (1), 65-70 (2015).</li><li>Nykonenko, A., V&#225;vra, P., Zon&#269;a, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Anatomic+Peculiarities+of+Pig+and+Human+Liver.">Anatomic Peculiarities of Pig and Human Liver.</a> Exp Clin Transplant Off J Middle East Soc Organ Transplant. 15 (1), 21-26 (2017).</li><li>Fechner, G., von Pezold, J., Luzar, O., Hauser, S., Tolba, R. H., M&#252;ller, S. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Modified+spectrometry+(O2C+device)+of+intraoperative+microperfusion+predicts+organ+function+after+kidney+transplantation:+a+pilot+study.">Modified spectrometry (O2C device) of intraoperative microperfusion predicts organ function after kidney transplantation: a pilot study.</a> Transplant Proc. 41 (9), 3575-3579 (2009).</li><li>Patterson, E. J., Scudamore, C. H., Owen, D. A., Nagy, A. G., Buczkowski, A. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Radiofrequency+ablation+of+porcine+liver+in+vivo:+effects+of+blood+flow+and+treatment+time+on+lesion+size.">Radiofrequency ablation of porcine liver in vivo: effects of blood flow and treatment time on lesion size.</a> AnnSurg. 227, 559-565 (1998).</li></ol>

Gnager modeller for leveren kirurgi er etablert for en lang tid16. Likevel store dyr modeller har visse fordeler: ingen Mikrokirurgiske utstyr er nødvendig som operative standardutstyr for mennesker kan brukes og kirurgiske teknikker kan sammenlignes med klinisk bruk standard klinisk evaluering kan være overført til eksperimenter. Standard klinisk blodprøver kan for eksempel utføres uten behov for spesielle laboratorium testmetoder (Figur 10).
<p class="jove_...

Argon plasma koagulering (APC) er en mye brukt instrument i abdominal kirurgi for mer enn tre tiår1,2. Det er en standard teknikk for oppnåelse av sekundær hemostasen etter store hepatectomy forsegle leveren kutte overflate for å hindre senere hemorrhages3. Plasma koagulering er en spesialisert form for radiofrekvens electrocautery, som gir elektrisk energi gjennom en bue ionisert gass. Gir monopolar elektrotermiske hemostasen, har denne noncontact teknikken fordelen av hindre elektroden å stikke til vev4. Ionisert gass strålen sendes automatisk til området i den laveste motstanden og vendt bort når motstand stiger skyldes uttørking til andre områder ikke desiccated. Dette gir en ensartet begrenset dybdeskarphet koagulering5,6. Faktorer som påvirker koagulering effekten er aktiviseringen tid, strøminnstillingen koagulering enheten og avstanden fra sonden til vev. Helium er en annen transportør gass, som kan brukes for plasma koagulering7. Nylig kliniske studier konsentrert om kliniske utfall i stedet for histologiske og funksjonelle funn3,8,9, mens eksperimentelle studier fokuserte på i vitro undersøkelser10 eller eksperimenter på isolerte perfused organer11.
Den underliggende protokollen tillater studier av effekten av plasma koagulering i en stor dyremodell nær klinisk programmet bruker standard menneskelige utstyr på griser: mikrosirkulasjonen vurderes noninvasively av en laser Doppler flowmeter og spektrofotometer, som er en klinisk standardverktøy for dette indikasjon12,13. Temperaturendringer under koagulasjon overvåkes med en infrarød termometer og en Termografiske kamera. Dybden av koagulering måles på histologiske hematoxylin/eosin farget deler etter høsting av vevsprøver. For sammenligning med andre former for sekundær hemostasen utføres burst press eksperimenter. I motsetning til tidligere beskrevet teknikker14, dette er gjennomført på explanted organer å ekskludere blodtrykk relaterte effektene. I tillegg til beskrevet undersøkelser på lokale effekter av plasma koagulering, kan standard blodprøver også foretas i porcine modell.

<table border="0" cellpadding="0" cellspacing="0" width="824">
	<tbody>
		<tr height="47">
			<td height="47" style="height:47px;width:216px;">Xylazine 20 mg/mL</td>
			<td style="width:184px;">Vetoquinol GmbH</td>
			<td style="width:192px;">Xylapan</td>
			<td style="width:232px;"></td>
		</tr>
		<tr height="47">
			<td height="47" style="height:47px;width:216px;">Ketamine 100 mg/mL</td>
			<td style="width:184px;">Ceva GmbH</td>
			<td style="width:192px;">Ceva Ketamine Injection</td>
			<td style="width:232px;"></td>
		</tr>
		<tr height="47">
			<td height="47" style="height:47px;width:216px;">Atropine 100 mg / 10 mL</td>
			<td style="width:184px;">Dr. Franz K&#246;hler Chemie GmbH</td>
			<td style="width:192px;">Atropinsulfat K&#246;hler 100mg Amp.</td>
			<td style="width:232px;"></td>
		</tr>
		<tr height="47">
			<td height="47" style="height:47px;">Propofol</td>
			<td style="width:184px;">Fresenius Kabi GmbH</td>
			<td style="width:192px;">Propofol 1% MCT Fresenius</td>
			<td style="width:232px;"></td>
		</tr>
		<tr height="47">
			<td height="47" style="height:47px;">Fentanyl</td>
			<td style="width:184px;">KG Rotexmedica GmbH</td>
			<td style="width:192px;">Fentanyl 0,5mg Rotexmedica</td>
			<td style="width:232px;"></td>
		</tr>
		<tr height="47">
			<td height="47" style="height:47px;">Isoflurane</td>
			<td style="width:184px;">Abbot GmbH</td>
			<td style="width:192px;">Forene 100% (V/V) 250 mL</td>
			<td style="width:232px;"></td>
		</tr>
		<tr height="47">
			<td height="47" style="height:47px;">Ringer&#39;s lactate solution</td>
			<td style="width:184px;">Baxter Deutschland GmbH</td>
			<td style="width:192px;"></td>
			<td style="width:232px;">sodium 131mmol/l, potassium 5 mmol/l, calcium 2 mmol/l, cloride 111 mmol/l, lactate 29 mmol/l</td>
		</tr>
		<tr height="47">
			<td height="47" style="height:47px;width:216px;">Surgical disinfactant</td>
			<td style="width:184px;">Sch&#252;lke &#38; Mayr GmbH</td>
			<td style="width:192px;">Kodan Tinktur forte gef&#228;rbt 1l 104804</td>
			<td style="width:232px;"></td>
		</tr>
		<tr height="47">
			<td height="47" style="height:47px;width:216px;">Motorized microscope</td>
			<td style="width:184px;">Nikon Instruments Europe</td>
			<td style="width:192px;">Eclipse TE2000-E</td>
			<td style="width:232px;"></td>
		</tr>
		<tr height="47">
			<td height="47" style="height:47px;width:216px;">Microscope camera</td>
			<td style="width:184px;">Nikon Instruments Europe</td>
			<td style="width:192px;">Digitalsight DS-Qi1Mc</td>
			<td style="width:232px;"></td>
		</tr>
		<tr height="47">
			<td height="47" style="height:47px;width:216px;">Imaging software</td>
			<td style="width:184px;">Nikon Instruments Europe</td>
			<td style="width:192px;">NIS elements Vers. 4.40</td>
			<td style="width:232px;"></td>
		</tr>
		<tr height="47">
			<td height="47" style="height:47px;width:216px;">Plasma coagulator</td>
			<td style="width:184px;">S&#246;ring GmbH</td>
			<td style="width:192px;">CPC-1000</td>
			<td style="width:232px;"></td>
		</tr>
		<tr height="47">
			<td height="47" style="height:47px;width:216px;">Argon gas</td>
			<td style="width:184px;">Linde AG</td>
			<td style="width:192px;">Argon 4.8&#160;</td>
			<td style="width:232px;"></td>
		</tr>
		<tr height="47">
			<td height="47" style="height:47px;width:216px;">Helium gas</td>
			<td style="width:184px;">Linde AG</td>
			<td style="width:192px;">Helium 4.8</td>
			<td style="width:232px;"></td>
		</tr>
		<tr height="47">
			<td height="47" style="height:47px;width:216px;">O2C</td>
			<td style="width:184px;">LEA Medizintechnik GmbH</td>
			<td style="width:192px;">O2C Version 1212</td>
			<td style="width:232px;">with LF-2 or LF-3 probe</td>
		</tr>
		<tr height="47">
			<td height="47" style="height:47px;">Infrared thermometer</td>
			<td style="width:184px;">Voltcraft</td>
			<td>VOLTCRAFT IR 260-8S</td>
			<td style="width:232px;"></td>
		</tr>
		<tr height="47">
			<td height="47" style="height:47px;width:216px;">Thermographic camera</td>
			<td>InfraTec GmbH</td>
			<td>VarioCAM HD head 820</td>
			<td style="width:232px;"></td>
		</tr>
		<tr height="47">
			<td height="47" style="height:47px;width:216px;">Thermographic analysis sofrtware</td>
			<td>InfraTec GmbH</td>
			<td style="width:192px;">IRBIS 3</td>
			<td style="width:232px;"></td>
		</tr>
		<tr height="47">
			<td height="47" style="height:47px;width:216px;">Mayer&#39;s Hematoxylin solution</td>
			<td></td>
			<td style="width:192px;">Merck 1.09249</td>
			<td style="width:232px;"></td>
		</tr>
		<tr height="47">
			<td height="47" style="height:47px;width:216px;">Eosin solution</td>
			<td style="width:184px;">VWR International GmbH</td>
			<td style="width:192px;">Merck 1.09844</td>
			<td style="width:232px;"></td>
		</tr>
		<tr height="47">
			<td height="47" style="height:47px;width:216px;">Rollerpump Masterflex L/S easy Load</td>
			<td style="width:184px;">Cole-Parmer Instrument Company</td>
			<td style="width:192px;">model 7518-10</td>
			<td style="width:232px;"></td>
		</tr>
		<tr height="47">
			<td height="47" style="height:47px;width:216px;">Perfusorpump</td>
			<td style="width:184px;">B. Braun Melsungen AG</td>
			<td style="width:192px;">Perfusor secura FT</td>
			<td style="width:232px;"></td>
		</tr>
		<tr height="47">
			<td height="47" style="height:47px;width:216px;">Digital pressure meter</td>
			<td style="width:184px;">Greisinger electronic</td>
			<td style="width:192px;">GMH 3161</td>
			<td style="width:232px;"></td>
		</tr>
		<tr height="47">
			<td height="47" style="height:47px;width:216px;">Perfusorsyringe, 50 mL</td>
			<td style="width:184px;">B. Braun Melsungen AG</td>
			<td style="width:192px;">REF 8728810 F</td>
			<td style="width:232px;"></td>
		</tr>
		<tr height="47">
			<td height="47" style="height:47px;width:216px;">Perfusor line, Type IV Standard, PVC Luer lock</td>
			<td style="width:184px;">B. Braun Melsungen AG</td>
			<td style="width:192px;">REF 8722960</td>
			<td style="width:232px;"></td>
		</tr>
		<tr height="47">
			<td height="47" style="height:47px;width:216px;">3-Way stopcock, Dicofix C35C</td>
			<td style="width:184px;">B. Braun Melsungen AG</td>
			<td style="width:192px;">REF 16494 C</td>
			<td style="width:232px;"></td>
		</tr>
		<tr height="47">
			<td height="47" style="height:47px;width:216px;">Silk 2-0. 3 metric</td>
			<td style="width:184px;">Resorba</td>
			<td style="width:192px;">REF H5F</td>
			<td style="width:232px;"></td>
		</tr>
		<tr height="47">
			<td height="47" style="height:47px;width:216px;">Vicryl 4-0 Sutupak</td>
			<td style="width:184px;">Ethicon</td>
			<td>V1224H</td>
			<td style="width:232px;"></td>
		</tr>
		<tr height="47">
			<td height="47" style="height:47px;width:216px;">NaCl 0.9 %</td>
			<td style="width:184px;">B. Braun Melsungen AG</td>
			<td style="width:192px;"></td>
			<td style="width:232px;"></td>
		</tr>
	</tbody>
</table>

assessment of plasma coagulation on liver tissue in a large animal model in vivo

Plasma koagulering som en form for electrocautery brukes i leveren kirurgi i flere tiår for å forsegle store leveren kuttet overflaten etter store hepatectomy å hindre blødninger på et senere tidspunkt. Eksakt effekten av plasma koagulering på leveren vev er bare dårlig undersøkt. I vår porcine modell, kan koagulering effektene undersøkes nær klinisk anvendelse. En kombinert laser Doppler flowmeter og spektrofotometer dokumenter mikrosirkulasjonen endres under koagulering på 8 mm vev dyp noninvasively, å gi kvantifiserbare informasjon om hemostasen utover subjektive klinisk inntrykk. Temperaturen på koagulering området vurderes med en infrarød termometer før og etter koagulering og en Termografiske kamera under koagulasjon, en måling av gass strålen temperaturen er ikke mulig på grunn av øvre terskelen til enhetene. Dybden av koagulering måles mikroskopisk på hematoxylin/eosin farget deler etter kalibrering med et objekt mikrometer og gir en nøyaktig informasjon om makt innstillingen-coagulation dybde-forholdet. Tetting effekten undersøkes på galle kanaler er det ikke mulig for en plasma coagulator å forsegle større blodkar. Burst press eksperimenter er gjennomført ut på explanted organer å utelukke blodtrykk relaterte effektene.

Mikrosirkulasjonen: Utnytte diagnostiske enheten etter plasma koagulering hemostasen kan påvises av mikrosirkulasjonen endringer. Kapillær blodet flyt (vises som vilkårlig enheter (AU)) reduseres fra planlagte verdien 142.7 ± 76.08 AU 57.78 ± 49.57 AU på 25 W enheten utgangseffekt, til 48.5 ± 7.26 AU på 50 M og ca 5,04 ± 1,31 AU 100 W (Figur 4).
Temperatur:...

Watch this Scientific Journal Video about Assessment of Plasma Coagulation on Liver Tissue in a Large Animal Model In Vivo at JoVE.com

Assessment of Plasma Coagulation on Liver Tissue in a Large Animal Model In Vivo

Her presenterer vi en protokoll for å vurdere eksperimentelt plasma koagulering i leveren vev i vivo. I en porcine modell mikrosirkulasjonen undersøkes av laser Doppler, koagulering dybde måles histologisk, temperaturen koagulering området av infrarød termometer Termografiske kamera og rør tetting effekt er dokumentert av burst press eksperimenter.

Vurdering av Plasma koagulering på leveren vev i en stor dyremodell i Vivo

Plasma coagulation as a form of electrocautery is used in liver surgery for decades to seal the large liver cut surface after major hepatectomy to prevent ...

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JoVE Journal

Medicine

Assessment of Plasma Coagulation on Liver Tissue in a Large Animal Model In Vivo

An Isolated Working Heart System for Large Animal Models

Et isolert Working Hjerte System for stort dyr modeller

Since its introduction in the late 19th century, the Langendorff isolated heart perfusion apparatus, and the subsequent development of the working heart ...

Depletion of Mouse Cells from Human Tumor Xenografts Significantly Improves Downstream Analysis of Target Cells

Nedbryting av museceller fra menneske tumorxenotransplantater forbedrer Nedstrøms Analyse av Target Cells

The use of in vitro cell line models for cancer research has been a useful tool. However, it has been shown that these models fail to reliably mimic ...

Assessment of Child Anthropometry in a Large Epidemiologic Study

Vurdering av Child Antropometri i en stor Epidemiologiske Study

A high proportion of children have overweight and obesity in the United States and other countries. Accurate assessment of anthropometry is essential to ...

Surgical Angiogenesis in Porcine Tibial Allotransplantation: A New Large Animal Bone Vascularized Composite Allotransplantation Model

Kirurgisk angiogenese i svin Tibial Allotransplantation: en ny stor dyr bein Stangeriaceae sammensatte Allotransplantation modell

Segmental bone loss resulting from trauma, infection malignancy and congenital anomaly remains a major reconstructive challenge. Current therapeutic ...

Invasive Hemodynamic Monitoring of Aortic and Pulmonary Artery Hemodynamics in a Large Animal Model of ARDS

Invasiv Hemodynamic overvåking av aorta og pulmonal arterien Hemodynamics i en stor dyr modell av ARDS

One of the leading causes of morbidity and mortality in patients with heart failure is right ventricular (RV) dysfunction, especially if it is due to ...

A Small Animal Model of Ex Vivo Normothermic Liver Perfusion

A Small Animal Model of Ex Vivo Normothermic Liver Perfusion

En liten dyr modell av Ex Vivo Normothermic lever perfusjon

There is a significant shortage of liver allografts available for transplantation, and in response the donor criteria have been expanded. As a result, ...

Induction and Phenotyping of Acute Right Heart Failure in a Large Animal Model of Chronic Thromboembolic Pulmonary Hypertension

Induksjon og fenotyping av akutt høyre hjertesvikt i en stor dyremodell av kronisk tromboembolisk lungehypertensjon

The development of acute right heart failure (ARHF) in the context of chronic pulmonary hypertension (PH) is associated with poor short-term outcomes. The ...

Isometric Contractility Measurement of the Mouse Mesenteric Artery Using Wire Myography

Isometrisk Contractility måling av musen hvem arterien bruker Wire Myography

The wire myograph technique is used to assess the contractility of vascular smooth muscles in response to depolarization, GPCR agonists/inhibitors and ...

In Vitro Model of Coronary Angiogenesis

In Vitro Modell av Koronar Angiogenese

Here, we describe an in vitro culture assay to study coronary angiogenesis. Coronary vessels feed the heart muscle and are of clinical importance. Defects ...

Using a Chemical Biopsy for Graft Quality Assessment

Bruke en kjemisk biopsi for graftkvalitetsvurdering

Kidney transplantation is a life-saving treatment for a large number of people with end-stage renal dysfunction worldwide. The procedure is associated ...