Name: a rat model of central fatigue using a modified multiple platform method
Uploaded: 2018-08-14T13:30:00
Description: Watch this Scientific Journal Video about A Rat Model of Central Fatigue Using a Modified Multiple Platform Method at JoVE.com

Dette arbeidet ble støttet av Natural Science Foundation av Beijing (No.7162124) og Xin-ao grunnlaget for Beijings universitet for kinesisk medisin.

Alle dyrene ble opprettholdt i henhold til veiledning av kinesiske lovgivningen på etisk bruk og omsorg forsøksdyr.
1. pre modellering forberedelse
<ol><li>
Laboratoriet forberedelse
	<ol>
<li>Kjør UV-lampen i minst 30 min før eksperimentet.</li>
		<li>Kontrollere lab temperaturen på 25 ± 3 ° C, og relativ luftfuktighet rundt 30%.</li>
		<li>Slå på laboratoriet lys på 6:00 og slå den av på 18:00 å etablere en 12 h/12 h lys/mørke syklus.</li>
	</ol>
</li>
	<li>
...

<ol>
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L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Grooming+analysis+algorithm:+Use+in+the+relationship+between+sleep+deprivation+and+anxiety-like+behavior.">Grooming analysis algorithm: Use in the relationship between sleep deprivation and anxiety-like behavior.</a> Progress in Neuro-Psychopharmacology and Biological Psychiatry. 41, 6-10 (2013).</li><li>Yamashita, M., Yamamoto, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Establishment+of+a+rat+model+of+central+fatigue+induced+by+chronic+sleep+disorder+and+excessive+brain+tryptophan.">Establishment of a rat model of central fatigue induced by chronic sleep disorder and excessive brain tryptophan.</a> Japanese Journal of Cognitive Neuroscience. 15, 67-74 (2013).</li><li>Arai, M., Yamazaki, M., Inoue, K., Fushiki, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+intracranial+injection+of+transforming+growth+factor-beta+relevant+to+central+fatigue+on+the+waking+electroencephalogram+of+rats+Comparison+with+effects+of+exercise.">Effects of intracranial injection of transforming growth factor-beta relevant to central fatigue on the waking electroencephalogram of rats Comparison with effects of exercise.</a> Progress in Neuro-Psychopharmacology and Biological Psychiatry. 26 (2), 307-312 (2002).</li><li>Han, C. X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Distinct+behavioral+and+brain+changes+after+different+durations+of+the+modified+multiple+platform+method+on+rats:+An+animal+model+of+central+fatigue.">Distinct behavioral and brain changes after different durations of the modified multiple platform method on rats: An animal model of central fatigue.</a> PloS One. 12 (5), e0176850 (2017).</li><li>Tang, X., Yang, L., Sanford, L. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Individual+variation+in+sleep+and+motor+activity+in+rats.">Individual variation in sleep and motor activity in rats.</a> Behavioural Brain Research. 180 (1), 62-68 (2007).</li><li>Stanford, S. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Open+Field+Test:+reinventing+the+wheel.">The Open Field Test: reinventing the wheel.</a> Journal of Psychopharmacology. 21 (2), 134-135 (2007).</li><li>Ahn, S. H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Basal+anxiety+during+an+open+field+test+is+correlated+with+individual+differences+in+contextually+conditioned+fear+in+mice.">Basal anxiety during an open field test is correlated with individual differences in contextually conditioned fear in mice.</a> Animal Cells and Systems. 17 (3), 154 (2013).</li><li>Costa, A. A., Morato, S., Roque, A. 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MMPM er konstruert for søvn deprivasjon9. Rotter er lansert i en vanntank med plattformer på bunnen. Drevet av instinktiv frykt for vann, rotter forbli stående på plattformer og ingen søvn forekommer. Studien viser at ulike tider av søvnmangel føre til ulike endringer i rotte atferd og humør, inkludert anerkjennelse verdifall10, negative følelser11og sentral fatigue. Noen forskere bevise at kronisk søvnmangel med metoden enkelt plattform (S...

Trøtthet er en av de viktigste faktorene som truer helse1. I de siste tiårene, har ulike undersøkelser vist at trøtthet er perifert utløst men sentralt-drevet og alltid sammen med emosjonelle og kognitive lidelser. Italiensk fysiolog A. Mosso først foreslått ordet sentral Fatigue2. Det er vanligvis definert som begrenset frivillig aktivitet og kognisjon svekkelse på grunn av dysfunksjon av impuls overføring i sentralnervesystemet (CNS)3. Sammenlignet med eksterne muskeltretthet, fremhever sentral fatigue endringer i CNS, samt den påfølgende emosjonelle/atferdsmessige forstyrrelser, inkludert depresjon, angst, kognisjon verdifall og hukommelsestap. En studie viser at mange faktorer kan indusere sentral fatigue, blant som overdreven fysisk aktivitet og psykisk stress er helt uunnværlig4. Som for patogenesen forklare teorier som tryptofan-kynurenine veien hypotesen5 endringer i bestemte baner; grundigere studier er imidlertid fremdeles nødvendig å avsløre sentral-tilleggsutstyr sammenhenger av sentral fatigue.
Den underliggende mekanismen av sentral fatigue er fortsatt uklart, er en effektiv dyremodell ganske viktig for videre forskning. Eksisterende tretthet modeller er hovedsakelig forårsaket av overdreven trening, som tredemøller6 og vekt-lastet svømming7, med liten bekymring på mental faktorer. For å bedre simulere utviklingen av sentral fatigue, utviklet vår gruppe en rotte modell med MMPM. Under modelleringsprosessen forbli rotter stående på smale plattformer i boksen mangfoldig plattform for lange timer del av dvaleinnstillinger. Forskjellig fra overdreven trening modeller, bruker MMPM modellen delvis søvnmangel som en mental faktor behandling komplekse patogenesen av sentral fatigue.
Modell evaluering bruker vi ofte og EPM-tester til å bestemme angst humør og frivillig aktivitet. ES testen utføres for å måle perifere muskel ytelsen. Dessuten, vi tar rottas hjernen og oppdage dopamin (DA) / serotonin (5-HT) innhold i begge hypothalamuses å observere sentrale nevrotransmitter forskjellene.
Protokollen nedenfor er beregnet på modellen sentral fatigue indusert av gjentatte fysiske aktivitet og mangel på søvn, etterligne en vanlig tilstand i menneskers liv. Imidlertid ved å justere hele modellen, kan den brukes i mange andre felt, som i søvn observasjon og stress studier. I fremtid forskning, vi håper at denne modellen vil hjelpe oppdage flere CNS endringer og deres forbindelse med eksterne systemet, å avsløre patogenesen mekanisme sentral fatigue.

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>multiple platform sleep deprivation water tank</td>
			<td>Customization,it is provided by the neuroimmunological laboratory of Beijing University of Chinese Medicine</td>
			<td></td>
			<td>110cm x 60cm x 40cm. There are 15 plastic small platforms at the bottom. The small platform is 6.5cm in diameter and 8cm high</td>
		</tr>
		<tr>
			<td>Wistar rats</td>
			<td>Beijing Weitong Lihua Experimental Animal Technology Company</td>
			<td>license number SYXK (Beijing) 2016-0011</td>
			<td>Use 32 Wistar healthy male rats ,8 week old (200-210 g)</td>
		</tr>
		<tr>
			<td>Agilent 1100LC high performance liquid chromatograph&#160;</td>
			<td>Agilent</td>
			<td>&#160;G1379A, G1311A, G1313A , G1316A&#160;</td>
			<td>&#160;G1379A, G1311A type chromatographic pump, G1313A automatic sampler, G1316A column temperature box</td>
		</tr>
		<tr>
			<td>DECADE II SDC electrochemical detector</td>
			<td>Dutch ANTEC company</td>
			<td></td>
			<td>glassy carbon electrode, Ag/AgCl reference electrode, workstations (Clarity CHS)</td>
		</tr>
		<tr>
			<td>Biofuge Stratos high-speed refrigeration centrifuge</td>
			<td>HERAEUS</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>VCX130 ultrasonic fracturing instrument</td>
			<td>SONICS</td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>ACS-ZEAS electronic scale</td>
			<td>Phos technology development, Beijing.</td>
			<td></td>
			<td>The weight of the weighing rats can be accurate to 0.1g.</td>
		</tr>
		<tr>
			<td>Open Field Box</td>
			<td>Customization,it is provided by the neuroimmunological laboratory of Beijing University of Chinese Medicine</td>
			<td></td>
			<td>wooden box of open field&#160; 100 cm by 100 cm x 40 cm, inside wall and bottom as the gray.The bottom is divided into 25 equal area squares, each of which is 20cm x 20cm, and the 16 grids along the outer wall are the external ones, and the other 9 grids are central.The camera is mounted above the median.</td>
		</tr>
		<tr>
			<td>Elevated Plus-maze</td>
			<td>Beijing zhongshi dechuang technology development co. LTD.</td>
			<td></td>
			<td>The open arms and close&#160; arms of the cross are composed of 30cm x 5cm x 15cm, and the central area is 5cm x 5cm, with a camera mounted above the center and 45cm high.</td>
		</tr>
		<tr>
			<td>rat swimming bucket.</td>
			<td>Zhenhua biological instrument equipment co., LTD. Anhui,China.</td>
			<td></td>
			<td>The volume of plastic drum is 70cm x 30cm x 110cm, which is used for swimming in rats.</td>
		</tr>
		<tr>
			<td>Thermometer</td>
			<td>Shiya instrument co., LTD., changzhou,China.</td>
			<td></td>
			<td>Control water temperature</td>
		</tr>
		<tr>
			<td>Small water pump</td>
			<td>Xincheng technology co., LTD., chengdu,China.</td>
			<td></td>
			<td>Used for water tank and swimming behavior.</td>
		</tr>
		<tr>
			<td>Ethovition3.0 behavioral software.</td>
			<td>Nuldus,Netherlands</td>
			<td></td>
			<td>Measurement analysis of rat behavior videos.</td>
		</tr>
	</tbody>
</table>

a rat model of central fatigue using a modified multiple platform method

I denne artikkelen vi innført en rotte modell av sentral fatigue bruker endret flere plattform metoden (MMPM). Boksen flere plattformen ble utformet som en vanntank med smale plattformer på bunnen. Modell rotter ble satt på tanken og sto på plattformene for 14 h (18:00-8:00) per dag for en 21 dager, med en tom kontrollgruppe angitt for kontrast. På slutten av modellering, rotter i modellgruppen viste en tydelig trett utseende. For å vurdere modellen, vi utført flere atferdsmessige tester, inkludert feltet åpen test (ofte), forhøyet pluss labyrinten (EPM) test, og uttømmende svømming (ES) testen. Resultatene viste at angst, romlig kognisjon svekkelse, fattige muskel ytelse og avslått frivillig aktivitet i modellen rotter bekrefte diagnosen sentral fatigue. Endringer av de sentrale nevrotransmittere også bekreftet resultatet. Avslutningsvis modellen er simulert sentral fatigue, og fremtidige studie med modellen kan bidra til å avsløre patologisk mekanismen av sykdommen.

Vi beskriver en rotte modell av sentral fatigue bruker MMPM. 24 Wistar rotter er tilfeldig delt i kontrollgruppen og modellgruppen 12 rotter i hver gruppe. Modell apparatet er utformet som en vanntank med smale plattformer på bunnen (figur 1). Modell rotter stå på plattformene 14 h per dag, inkludert delvis sove tid, for 21 dager (figur 2).
Atferdsmessige testene utf...

Watch this Scientific Journal Video about A Rat Model of Central Fatigue Using a Modified Multiple Platform Method at JoVE.com

A Rat Model of Central Fatigue Using a Modified Multiple Platform Method

Her presenterer vi en protokoll for å innføre en rotte modell av sentral fatigue bruker endret flere plattform metoden (MMPM).

En rotte modell av sentral Fatigue bruker en modifisert flere plattform-metoden

In this article, we introduced a rat model of central fatigue using the modified multiple platform method (MMPM). The Multiple Platform box was designed ...

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