JoVE Logo
Faculty Resource Center

Sign In

Recombinant Collagen I Peptide Microcarriers for Cell Expansion and Their Potential Use As Cell Delivery System in a Bioreactor Model

DOI :

10.3791/57363-v

February 7th, 2018

February 7th, 2018

7,380 Views

1Department Tissue Engineering and Regenerative Medicine, University Hospital Wuerzburg, 2Translational Center Regenerative Therapies (TLC-RT), Fraunhofer Institute for Silicate Research ISC, 3Fujifilm Manufacturing Europe B.V.

We propose a cell expansion protocol on macroporous microcarriers and their use as delivery system in a perfusion bioreactor to seed a decellularized tissue matrix. We also include different techniques to determine cell proliferation and viability of cells cultured on microcarriers. Furthermore, we demonstrate functionality of cells after bioreactor cultures.

-- Views

Related Videos

article

Organotypic Collagen I Assay: A Malleable Platform to Assess Cell Behaviour in a 3-Dimensional Context

article

Rotating Cell Culture Systems for Human Cell Culture: Human Trophoblast Cells as a Model

article

Constructing a Collagen Hydrogel for the Delivery of Stem Cell-loaded Chitosan Microspheres

article

Skin Tattooing As A Novel Approach For DNA Vaccine Delivery

article

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation

article

Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform

article

An Improved Method for the Preparation of Type I Collagen From Skin

article

Cultivation of Mammalian Cells Using a Single-use Pneumatic Bioreactor System

article

Minced Tissue in Compressed Collagen: A Cell-containing Biotransplant for Single-staged Reconstructive Repair

article

Fabrication of Extracellular Matrix-derived Foams and Microcarriers as Tissue-specific Cell Culture and Delivery Platforms

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved