Name: generation of large numbers of myeloid progenitors and dendritic cell precursors from murine bone marrow using a novel cell sorting strategy
Uploaded: 2018-08-10T13:30:00
Description: Watch this Scientific Journal Video about Generation of Large Numbers of Myeloid Progenitors and Dendritic Cell Precursors from Murine Bone Marrow Using a Novel Cell Sorting Strategy at JoVE.com

我们感谢在奥本大学兽医医学流式细胞仪设施的艾莉森教堂鸟的技术援助, 从 NIH 到 EHS R15 R15 AI107773, 以及在奥本大学的细胞和分子生物学项目的资助为夏季研究基金提供资金。

所有动物工作都是由奥本大学机构动物保育和使用委员会根据《国家卫生研究院实验室动物护理和使用指南》中概述的建议批准的。
1. 骨髓收集的准备工作
<ol><li>准备250毫升完整的媒体, 通过添加一个解决方案的罗斯威尔公园纪念研究所 (RPMI) 1640 培养基补充10% 胎小牛血清, 2 毫米谷氨酰胺, 50 µM 2 巯基乙醇到0.22 µM 真空过滤瓶单元顶部, 并应用真空。 注: 完全培养基可储存...

<ol>
	<li>Zhan, Y., Xu, Y., Lew, A. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+regulation+of+the+development+and+function+of+dendritic+cell+subsets+by+GM-CSF:+more+than+a+hematopoietic+growth+factor.">The regulation of the development and function of dendritic cell subsets by GM-CSF: more than a hematopoietic growth factor.</a> Mol Immunol. 52, 30-37 (2012).</li><li>Zhan, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+inflammatory+cytokine,+GM-CSF,+alters+the+developmental+outcome+of+murine+dendritic+cells.">The inflammatory cytokine, GM-CSF, alters the developmental outcome of murine dendritic cells.</a> Eur J Immunol. , (2012).</li><li>van de Laar, L., Coffer, P. J., Woltman, A. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Regulation+of+dendritic+cell+development+by+GM-CSF:+molecular+control+and+implications+for+immune+homeostasis+and+therapy.">Regulation of dendritic cell development by GM-CSF: molecular control and implications for immune homeostasis and therapy.</a> Blood. 119, 3383-3393 (2012).</li><li>Steinman, R. M., Inaba, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Myeloid+dendritic+cells.">Myeloid dendritic cells.</a> J Leukoc Biol. 66, 205-208 (1999).</li><li>Inaba, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Generation+of+large+numbers+of+dendritic+cells+from+mouse+bone+marrow+cultures+supplemented+with+granulocyte/macrophage+colony-stimulating+factor.">Generation of large numbers of dendritic cells from mouse bone marrow cultures supplemented with granulocyte/macrophage colony-stimulating factor.</a> J Exp Med. 176, 1693-1702 (1992).</li><li>Steinman, R. M., Pack, M., Inaba, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dendritic+cell+development+and+maturation.">Dendritic cell development and maturation.</a> Adv Exp Med Biol. , 1-6 (1997).</li><li>Pierre, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Developmental+regulation+of+MHC+class+II+transport+in+mouse+dendritic+cells.">Developmental regulation of MHC class II transport in mouse dendritic cells.</a> Nature. 388, 787-792 (1997).</li><li>Steinman, R. M., Witmer-Pack, M., Inaba, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dendritic+cells:+antigen+presentation,+accessory+function+and+clinical+relevance.">Dendritic cells: antigen presentation, accessory function and clinical relevance.</a> Adv Exp Med Biol. , 1-9 (1993).</li><li>Na, Y. R., Jung, D., Gu, G. J., Seok, S. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=GM-CSF+Grown+Bone+Marrow+Derived+Cells+Are+Composed+of+Phenotypically+Different+Dendritic+Cells+and+Macrophages.">GM-CSF Grown Bone Marrow Derived Cells Are Composed of Phenotypically Different Dendritic Cells and Macrophages.</a> Mol Cells. 39, 734-741 (2016).</li><li>Rogers, P. B., Driessnack, M. G., Hiltbold Schwartz, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Analysis+of+the+developmental+stages,+kinetics,+and+phenotypes+exhibited+by+myeloid+cells+driven+by+GM-CSF+in+vitro.">Analysis of the developmental stages, kinetics, and phenotypes exhibited by myeloid cells driven by GM-CSF in vitro.</a> PLoS One. 12, e0181985 (2017).</li><li>Helft, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=GM-CSF+Mouse+Bone+Marrow+Cultures+Comprise+a+Heterogeneous+Population+of+CD11c(+)MHCII(+)+Macrophages+and+Dendritic+Cells.">GM-CSF Mouse Bone Marrow Cultures Comprise a Heterogeneous Population of CD11c(+)MHCII(+) Macrophages and Dendritic Cells.</a> Immunity. 42, 1197-1211 (2015).</li><li>Alloatti, A., Kotsias, F., Magalhaes, J. G., Amigorena, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dendritic+cell+maturation+and+cross-presentation:+timing+matters!.">Dendritic cell maturation and cross-presentation: timing matters!.</a> Immunol Rev. 272, 97-108 (2016).</li><li>Jakubzick, C. V., Randolph, G. J., Henson, P. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Monocyte+differentiation+and+antigen-presenting+functions.">Monocyte differentiation and antigen-presenting functions.</a> Nat Rev Immunol. 17, 349-362 (2017).</li><li>Mbongue, J. C., Nieves, H. A., Torrez, T. W., Langridge, W. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Role+of+Dendritic+Cell+Maturation+in+the+Induction+of+Insulin-Dependent+Diabetes+Mellitus.">The Role of Dendritic Cell Maturation in the Induction of Insulin-Dependent Diabetes Mellitus.</a> Frontiers in immunology. 8, 327 (2017).</li><li>Shortman, K., Naik, S. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Steady-state+and+inflammatory+dendritic-cell+development.">Steady-state and inflammatory dendritic-cell development.</a> Nat Rev Immunol. 7, 19-30 (2007).</li><li>Xu, Y., Zhan, Y., Lew, A. M., Naik, S. H., Kershaw, M. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Differential+development+of+murine+dendritic+cells+by+GM-CSF+versus+Flt3+ligand+has+implications+for+inflammation+and+trafficking.">Differential development of murine dendritic cells by GM-CSF versus Flt3 ligand has implications for inflammation and trafficking.</a> J Immunol. 179, 7577-7584 (2007).</li><li>Naik, S. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Generation+of+large+numbers+of+pro-DCs+and+pre-DCs+in+vitro.">Generation of large numbers of pro-DCs and pre-DCs in vitro.</a> Methods Mol Biol. 595, 177-186 (2010).</li><li>Nikolic, T., de Bruijn, M. F., Lutz, M. B., Leenen, P. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Developmental+stages+of+myeloid+dendritic+cells+in+mouse+bone+marrow.">Developmental stages of myeloid dendritic cells in mouse bone marrow.</a> Int Immunol. 15, 515-524 (2003).</li><li>Hettinger, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Origin+of+monocytes+and+macrophages+in+a+committed+progenitor.">Origin of monocytes and macrophages in a committed progenitor.</a> Nat Immunol. 14, 821-830 (2013).</li><li>Fogg, D. K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+clonogenic+bone+marrow+progenitor+specific+for+macrophages+and+dendritic+cells.">A clonogenic bone marrow progenitor specific for macrophages and dendritic cells.</a> Science. 311, 83-87 (2006).</li><li>Traver, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Development+of+CD8{alpha}-Positive+Dendritic+Cells+from+a+Common+Myeloid+Progenitor.">Development of CD8{alpha}-Positive Dendritic Cells from a Common Myeloid Progenitor.</a> Science. 290, 2152-2154 (2000).</li></ol>

该协议促进了基因型脑脊液驱动的祖细胞和前体细胞类型的分离, 这些数字足以进行多种类型的分析, 包括生化化验、体外细胞功能的测定或体内灌注。这种方法代表单核细胞衍生的树突状细胞的发展, 使细胞在这一发展途径的早期可靠的分离和鉴定, 以及那些分化的细胞类型更常见的一个重要进展在先前的研究中被孤立。
以前的分离从骨髓中产生的祖细胞...

细胞因子-巨噬细胞集落刺激因子 (GM-CSF) 培养小鼠骨髓细胞的方法广泛应用于大数1的单核细胞衍生树突状细胞 (moDC; 也称为炎症 DC) , 2,3,4,5。这些细胞在树突状细胞 (DC) 功能6,7,8的各种研究中非常有用。通常, 这些小鼠骨髓细胞培养6-8 天, 然后用于研究树突状细胞功能5。这些文化长期被认为主要是同源的, 包括多数被区分的 moDC。最近, 很明显, 在这个6–8的天文化时期结束时, 确实有许多 moDC, 以及分化的单核细胞衍生巨噬细胞 (moMacs) 9,10,11的大子集。我们自己的研究进一步扩展了这些发现, 证明其他不发达细胞的子集, 如 moDC 前体 (moDP) 和单核, 在7天10的时间内仍然保持在低频率的文化中。因此, 利用这个系统产生的细胞来研究树突状细胞 (DC) 功能, 可以反映出比以前更广泛的细胞类型的反应。
我们从研究 GM-CSF 生成的 moDC 与这些细胞在分化的最后阶段12,13,14的功能上学到了很多。然而, 我们对这些细胞2、15、16的发展途径以及它们如何和何时表现出特定功能, 如: 对病原体相关分子的反应能力的了解明显较少。模式 (PAMPs), 吞噬功能, 抗原处理和表现13, 和抗菌活性。17报告了一项隔离大量常规 Flt3L-driven DC 祖和前体的议定书。利用羧基琥珀酰亚胺酯 (CFSE) 染色的骨髓细胞 (在 Flt3L 中跟踪分裂细胞) 和培养3天, 实现了这些不同种群的分离。细胞被耗尽的宗族阳性细胞, 并排序为祖和前体群体的基础上 CD11c 表达17。Leenen 组在 GM-脑脊液驱动培养中识别 DC 早期祖细胞的另一种方法是根据 CD31 和 Ly6C 18对单元进行分类。最初的目标是建立一个类似的方法来获得 GM-脑脊液驱动 moDC 的祖和前体。由于由 GM-CSF 产生的特定细胞类型, 我们根据在早期和后期的发展阶段所表达的分子表达方式, 调整了方法和排序策略。我们最终确定 Ly6C、CD115 (CSF-1 受体) 和 CD11c 是区分这些细胞类型10的最佳标记。
在这里, 我们提出了一个方法, 在几个不同的发展阶段的细胞分离的途径, 由 GM-脑脊液: 普通髓样祖 (CMP), 粒细胞巨噬祖 (GMP), 单核细胞源性巨噬细胞(MoMac) 和单核细胞衍生 DC (MoDC)。moMac 种群可以根据 MHC II 类表达的水平进一步隔离, 揭示了 moDC 前体种群 (moDP) 10。我们利用一种高速荧光活化细胞分选 (Ly6C) 策略来隔离这5种群, 并以表达 CD115 和 CD11c 为基础。然后, 我们展示了这些细胞在功能检测中的检查, 揭示了它们对玉兰刺激的反应。

<table>
	<tbody>
		<tr>
			<td>RPMI 1640</td>
			<td>Corning</td>
			<td>15-040-CV</td>
			<td></td>
		</tr>
		<tr>
			<td>Fetal Calf Serum (FCS)</td>
			<td>HyClone</td>
			<td>SV30014.04</td>
			<td>to supplement complete medium and FWB</td>
		</tr>
		<tr>
			<td>GlutaMAX</td>
			<td>Gibco</td>
			<td>35050</td>
			<td>to supplement complete medium</td>
		</tr>
		<tr>
			<td>2-mercaptoethanol (2-ME)</td>
			<td>MP Biomedical</td>
			<td>190242</td>
			<td>to supplement complete medium</td>
		</tr>
		<tr>
			<td>75mM Vacuum Filter</td>
			<td>Thermo Scientific</td>
			<td>156-4045</td>
			<td>to sterilize complete media</td>
		</tr>
		<tr>
			<td>ACK Lysis Buffer</td>
			<td>Lonza</td>
			<td>10-548E</td>
			<td>to lyse red blood cell</td>
		</tr>
		<tr>
			<td>HEPES buffer</td>
			<td>Corning</td>
			<td>21-020-CM</td>
			<td>to rescue leukocytes after red blood cell lysis</td>
		</tr>
		<tr>
			<td>Phosphate Buffered Saline (PBS), Dulbecco&#39;s</td>
			<td>Lonza</td>
			<td>17-512F</td>
			<td>must be endotoxin free; chilled at 4 &#176;C</td>
		</tr>
		<tr>
			<td>35&#181;m Cell filter</td>
			<td>Falcon</td>
			<td>352235</td>
			<td>to break apart clumps before running through cytometer.</td>
		</tr>
		<tr>
			<td>GM-CSF</td>
			<td>Biosource</td>
			<td>PMC2011</td>
			<td>usable concentration of 10ng/mL</td>
		</tr>
		<tr>
			<td>Tissue cultured treated plate</td>
			<td>VWR</td>
			<td>10062-896</td>
			<td>for bone marrow cells after harvest</td>
		</tr>
		<tr>
			<td>Anti-Ly6C, Clone HK1.4</td>
			<td>Biolegend</td>
			<td>128018</td>
			<td></td>
		</tr>
		<tr>
			<td>Anti-CD115, Clone AFS98</td>
			<td>Tonbo Bioscience</td>
			<td>20-1152-U100</td>
			<td></td>
		</tr>
		<tr>
			<td>Anti-CD11c, Clone HL3</td>
			<td>BD Biosciences</td>
			<td>557400</td>
			<td>to differeniate CMP and MoDCs</td>
		</tr>
		<tr>
			<td>MoFlo XPD Flow Cytometer</td>
			<td>Beckman Coulter</td>
			<td>ML99030</td>
			<td></td>
		</tr>
		<tr>
			<td>BD Accuri C6</td>
			<td>BD Biosciences</td>
			<td>660517</td>
			<td></td>
		</tr>
		<tr>
			<td>100% Ethanol</td>
			<td>Pharmco-Aaper</td>
			<td>111000200CSPP</td>
			<td></td>
		</tr>
		<tr>
			<td>60mm Petri Dish</td>
			<td>Corning, Inc</td>
			<td>353002</td>
			<td></td>
		</tr>
		<tr>
			<td>50mL Conical tube</td>
			<td>VWR</td>
			<td>21008-242</td>
			<td></td>
		</tr>
		<tr>
			<td>C57BL/6 Mice</td>
			<td>The Jackson Laboratory</td>
			<td>000664</td>
			<td>Female; 10-20 weeks old</td>
		</tr>
		<tr>
			<td>Biosafety Hood</td>
			<td>Thermo Scientific</td>
			<td>8354-30-0011</td>
			<td></td>
		</tr>
		<tr>
			<td>10mL Syringe</td>
			<td>BD Biosciences</td>
			<td>301604</td>
			<td></td>
		</tr>
		<tr>
			<td>23-gauge needle</td>
			<td>BD Biosciences</td>
			<td>305145</td>
			<td></td>
		</tr>
		<tr>
			<td>Centrifuge 5810&#160;R</td>
			<td>eppendorf</td>
			<td>22625501</td>
			<td></td>
		</tr>
		<tr>
			<td>FlowJo Software v10</td>
			<td>BD Biosciences</td>
			<td>Version 10</td>
			<td>flowjo.com</td>
		</tr>
	</tbody>
</table>

generation of large numbers of myeloid progenitors and dendritic cell precursors from murine bone marrow using a novel cell sorting strategy

细胞-巨噬细胞集落刺激因子 (GM-CSF) 在小鼠骨髓中产生的单核细胞来源的树突状体细胞 (moDC) 的培养最近被认为比以前所赞赏的多异质性。这些文化通常包含 moDC 和单核细胞衍生巨噬细胞 (moMac), 甚至一些较不发达的细胞, 如单核。本议定书的目标是提供一种一致的方法来识别和分离这些文化中存在的许多细胞类型, 以便它们的具体功能可以进一步研究。这里提出的分类策略将细胞先分为四种, Ly6C 和 CD115 的表达, 它们在转基因-脑脊液驱动的文化中发展的时候, 都是由细胞瞬时表达的。这四个种群包括普通髓样祖细胞或 CMP (Ly6C、CD115)、粒细胞/巨噬细胞祖或 GMP (Ly6C+、CD115)、单核细胞 (Ly6C+、CD115+) 和单核细胞衍生的巨噬菌或 moMac (Ly6C、CD115+)。CD11c 还添加到排序策略中, 以区分 Ly6C 中的两个种群, CD115-人口: CMP (CD11c) 和 moDC (CD11c+)。最后, 根据 MHC II. 类表达的水平, 在 Ly6C、CD115+ 人群中可以进一步区分两个种群。MoMacs 表达较低水平的 mhc ii 类, 而单核细胞衍生的 DC 前体 (moDP) 表达较高的 mhc ii. 类。这种方法可以可靠地隔离几个发育不同的种群, 这些数字足以用于各种功能和发展分析。我们强调一个这样的功能读数, 这些细胞类型的差异反应的刺激与病原体相关的分子模式 (PAMPs)。

为了尽可能多地进行分析, 可以根据前向和侧向散射来常规地选择可行的细胞, 不包括非常小和非常粒状的事件 (在图 1A中的所有点地块上都应用一个典型的门)。为了确定这种门控策略是否可靠地排除了死细胞, 我们染色了7氨基放线菌 D (7) (图 1B)。7AAD. 由于细胞膜通透性, 在死亡和死亡细胞中染色 DNA, 并被存活细胞排除。分析了?...

Watch this Scientific Journal Video about Generation of Large Numbers of Myeloid Progenitors and Dendritic Cell Precursors from Murine Bone Marrow Using a Novel Cell Sorting Strategy at JoVE.com

Generation of Large Numbers of Myeloid Progenitors and Dendritic Cell Precursors from Murine Bone Marrow Using a Novel Cell Sorting Strategy

在这里, 我们提供了一种方法, 以识别和隔离大量的 GM-脑脊液驱动髓细胞使用高速细胞排序。根据 Ly6C 和 CD115 的表达, 可以确定五种不同的种群 (常见髓样祖细胞、粒/巨噬细胞祖、单核细胞、单核细胞衍生巨噬细胞和单核细胞衍生的 DCs)。

利用新的细胞分选策略, 从小鼠骨髓中生成大量的髓样祖细胞和树突状体细胞前体

Cultures of monocyte-derived dendritic cells (moDC) generated from mouse bone marrow using Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) have ...

Research

JoVE Journal

Immunology and Infection

Generation and Labeling of Murine Bone Marrow-derived Dendritic Cells with Qdot Nanocrystals for Tracking Studies

小鼠骨髓来源的树突状细胞与跟踪研究的Qdot纳米晶的生成及标签

Dendritic cells (DCs) are professional antigen presenting cells (APCs) found in peripheral tissues and in immunological organs such as thymus, bone ...

科研

免疫与感染

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

使用骨髓源性巨噬细胞巨噬细胞极化的调查

The article describes a readily easy adaptive in vitro model to investigate macrophage polarization. In the presence of GM-CSF/M-CSF, hematopoietic ...

Generation of a Novel Dendritic-cell Vaccine Using Melanoma and Squamous Cancer Stem Cells

使用黑色素瘤和鳞状癌干细胞生成新型支气干细胞疫苗

We identified cancer stem cell (CSC)-enriched populations from murine melanoma D5 syngeneic to C57BL/6 mice and the squamous cancer SCC7 syngeneic to C3H ...

Isolation of Myeloid Dendritic Cells and Epithelial Cells from Human Thymus

从人类胸腺髓样树突状细胞和上皮细胞的分离

In this protocol we provide a method to isolate dendritic cells (DC) and epithelial cells (TEC) from the human thymus. DC and TEC are the major antigen ...

Assessing the Development of Murine Plasmacytoid Dendritic Cells in Peyer's Patches Using Adoptive Transfer of Hematopoietic Progenitors

评估小鼠浆细胞树突状细胞在淋巴集结的开发利用造血祖细胞的过继转移

This protocol details a method to analyze the ability of purified hematopoietic progenitors to generate plasmacytoid dendritic cells (pDC) in intestinal ...

Isolation and Intravenous Injection of Murine Bone Marrow Derived Monocytes

隔离和小鼠骨髓的静脉注射单核细胞衍生

As a subtype of leukocytes and progenitors of macrophages, monocytes are involved in many important processes of organisms and are often the subject of ...

In Vitro Generation of Murine Plasmacytoid Dendritic Cells from Common Lymphoid Progenitors using the AC-6 Feeder System

In Vitro Generation of Murine Plasmacytoid Dendritic Cells from Common Lymphoid Progenitors using the AC-6 Feeder System

在体外产生的常见淋巴祖使用AC-6馈线系统的小鼠浆细胞样树突细胞

Plasmacytoid dendritic cells (pDCs) are powerful type I interferon (IFN-I) producing cells that are activated in response to infection or during ...

Murine Hind Limb Long Bone Dissection and Bone Marrow Isolation

鼠后肢长骨解剖和骨髓隔离

Investigation of the bone and the bone marrow is critical in many research fields including basic bone biology, immunology, hematology, cancer metastasis, ...

Generation and Identification of GM-CSF Derived Alveolar-like Macrophages and Dendritic Cells From Mouse Bone Marrow

代和GM-CSF的鉴定源性肺泡状巨噬细胞和树突状细胞从小鼠骨髓

Macrophages and dendritic cells (DCs) are innate immune cells found in tissues and lymphoid organs that play a key role in the defense against pathogens. ...

Femur Window Chamber Model for In Vivo Cell Tracking in the Murine Bone Marrow

Femur Window Chamber Model for In Vivo Cell Tracking in the Murine Bone Marrow

股骨窗口商会模型<em&gt;在体内</em在小鼠骨髓&gt;手机跟踪

Bone marrow is a complex organ that contains various hematopoietic and non-hematopoietic cells. These cells are involved in many biological processes, ...