Name: electrically conductive scaffold to modulate and deliver stem cells
Uploaded: 2018-04-13T13:30:00
Description: Watch this Scientific Journal Video about Electrically Conductive Scaffold to Modulate and Deliver Stem Cells at JoVE.com

Vi takker Dr. Kati Andreasson (Institut for neurologi og neurologiske videnskab, Stanford University) for brug af qRT-PCR-maskine. Arbejdet var støttet af nationale institutter for sundhed tilskud K08NS098876 (til P.M.G.) og Stanford School of Medicine Dean postdoc stipendium (at bo).

Alle stamceller og dyre procedurer blev godkendt af Stanford's Stem Cell Research tilsyn udvalget og Stanford University's Administrative Panel for Laboratory Animal Care (SCRO-616 og APLAC-31909).
1. ætsning af ITO glas
<ol><li>Forberede indium tin oxid (ITO)-overdækket glas dias ved at placere en maskering agent over den ledende side af glasset. Bemærk: De fleste kommercielle transparente bånd kan bruges som en maskering agent.<ol>
<li>Fjern den overskydende maske ved hjælp af en v...

<ol>
	<li>Moskowitz, M. A., Lo, E. H., Iadecola, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Science+of+Stroke:+Mechanisms+in+Search+of+Treatments.">The Science of Stroke: Mechanisms in Search of Treatments.</a> Neuron. 67 (2), 181-198 (2010).</li><li>George, P. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Electrical+Preconditioning+of+Stem+Cells+with+a+Conductive+Polymer+Scaffold+Enhances+Stroke+Recovery.">Electrical Preconditioning of Stem Cells with a Conductive Polymer Scaffold Enhances Stroke Recovery.</a> Biomaterials. 142, 31-40 (2017).</li><li>Steinberg, G. K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Clinical+Outcomes+of+Transplanted+Modified+Bone+Marrow-Derived+Mesenchymal+Stem+Cells+in+Stroke:+A+Phase+1/2a+Study.">Clinical Outcomes of Transplanted Modified Bone Marrow-Derived Mesenchymal Stem Cells in Stroke: A Phase 1/2a Study.</a> Stroke. 47 (7), 1817-1824 (2016).</li><li>George, P. M., Steinberg, G. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Novel+Stroke+Therapeutics:+Unraveling+Stroke+Pathophysiology+and+Its+Impact+on+Clinical+Treatments.">Novel Stroke Therapeutics: Unraveling Stroke Pathophysiology and Its Impact on Clinical Treatments.</a> Neuron. 87 (2), 297-309 (2015).</li><li>Schabitz, W. R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intravenous+Brain-Derived+Neurotrophic+Factor+Enhances+Poststroke+Sensorimotor+Recovery+and+Stimulates+Neurogenesis.">Intravenous Brain-Derived Neurotrophic Factor Enhances Poststroke Sensorimotor Recovery and Stimulates Neurogenesis.</a> Stroke. 38 (7), 2165-2172 (2007).</li><li>Liauw, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Thrombospondins+1+and+2+Are+Necessary+For+Synaptic+Plasticity+and+Functional+Recovery+After+Stroke.">Thrombospondins 1 and 2 Are Necessary For Synaptic Plasticity and Functional Recovery After Stroke.</a> J Cereb Blood Flow Metab. 28 (10), 1722-1732 (2008).</li><li>Cantu, D. A., Hematti, P., Kao, W. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cell+Encapsulating+Biomaterial+Regulates+Mesenchymal+Stromal/Stem+Cell+Differentiation+and+Macrophage+Immunophenotype.">Cell Encapsulating Biomaterial Regulates Mesenchymal Stromal/Stem Cell Differentiation and Macrophage Immunophenotype.</a> Stem Cells Translational Medicine. 1 (10), 740-749 (2012).</li><li>Chaudhuri, O., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hydrogels+with+Tunable+Stress+Relaxation+Regulate+Stem+Cell+Fate+and+Activity.">Hydrogels with Tunable Stress Relaxation Regulate Stem Cell Fate and Activity.</a> Nat Mater. 15 (3), 326-334 (2016).</li><li>Ballios, B. G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+Hyaluronan-Based+Injectable+Hydrogel+Improves+the+Survival+and+Integration+of+Stem+Cell+Progeny+following+Transplantation.">A Hyaluronan-Based Injectable Hydrogel Improves the Survival and Integration of Stem Cell Progeny following Transplantation.</a> Stem Cell Reports. 4 (6), 1031-1045 (2015).</li><li>Mothe, A. J., Tam, R. Y., Zahir, T., Tator, C. H., Shoichet, M. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Repair+of+the+Injured+Spinal+Cord+by+Transplantation+of+Neural+Stem+Cells+in+a+hyaluronan-based+hydrogel.">Repair of the Injured Spinal Cord by Transplantation of Neural Stem Cells in a hyaluronan-based hydrogel.</a> Biomaterials. 34 (15), 3775-3783 (2013).</li><li>Saini, M., Singh, Y., Arora, P., Arora, V., Jain, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Implant+Biomaterials:+A+Comprehensive+Review.">Implant Biomaterials: A Comprehensive Review.</a> World J Clin Cases. 3 (1), 52-57 (2015).</li><li>Huang, Y., Li, Y., Chen, J., Zhou, H., Tan, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Electrical+Stimulation+Elicits+Neural+Stem+Cells+Activation:+New+Perspectives+in+CNS+Repair.">Electrical Stimulation Elicits Neural Stem Cells Activation: New Perspectives in CNS Repair.</a> Front Hum Neurosci. 9, (2015).</li><li>Pritchett-Corning, K. R., Mulder, G. B., Luo, Y., White, W. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Principles+of+Rodent+Surgery+for+the+New+Surgeon.">Principles of Rodent Surgery for the New Surgeon.</a> J Vis Exp. (47), (2011).</li><li>Abdelwahid, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Stem+Cell+Death+and+Survival+in+Heart+Regeneration+and+Repair.">Stem Cell Death and Survival in Heart Regeneration and Repair.</a> Apoptosis. 21 (3), 252-268 (2016).</li><li>Wang, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Clinical+Status+of+Stem+Cell+Therapy+for+Ischemic+Cardiomyopathy.">The Clinical Status of Stem Cell Therapy for Ischemic Cardiomyopathy.</a> Stem Cells Int. 2015, (2015).</li><li>George, P. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fabrication+and+Biocompatibility+of+Polypyrrole+Implants+Suitable+for+Neural+Prosthetics.">Fabrication and Biocompatibility of Polypyrrole Implants Suitable for Neural Prosthetics.</a> Biomaterials. 26 (17), 3511-3519 (2005).</li><li>Aravamudan, B., Thompson, M., Pabelick, C., Prakash, Y. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Brain-Derived+Neurotrophic+Factor+Induces+Proliferation+of+Human+Airway+Smooth+Muscle+Cells.">Brain-Derived Neurotrophic Factor Induces Proliferation of Human Airway Smooth Muscle Cells.</a> J Cell Mol Med. 16 (4), 812-823 (2012).</li><li>Lopes, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Thrombospondin+2+Regulates+Cell+Proliferation+Induced+by+Rac1+Redox-Dependent+Signaling.">Thrombospondin 2 Regulates Cell Proliferation Induced by Rac1 Redox-Dependent Signaling.</a> Mol Cell Biol. 23 (15), 5401-5408 (2003).</li><li>Ploughman, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Brain-Derived+Neurotrophic+Factor+Contributes+to+Recovery+of+Skilled+Reaching+After+Focal+Ischemia+in+Rats.">Brain-Derived Neurotrophic Factor Contributes to Recovery of Skilled Reaching After Focal Ischemia in Rats.</a> Stroke. 40 (4), 1490-1495 (2009).</li><li>Baker, E. W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Induced+Pluripotent+Stem+Cell-Derived+Neural+Stem+Cell+Therapy+Enhances+Recovery+in+an+Ischemic+Stroke+Pig+Model.">Induced Pluripotent Stem Cell-Derived Neural Stem Cell Therapy Enhances Recovery in an Ischemic Stroke Pig Model.</a> Sci Rep. 7 (1), 10075 (2017).</li><li>Bacigaluppi, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neural+Stem+Cell+Transplantation+Induces+Stroke+Recovery+by+Upregulating+Glutamate+Transporter+GLT-1+in+Astrocytes.">Neural Stem Cell Transplantation Induces Stroke Recovery by Upregulating Glutamate Transporter GLT-1 in Astrocytes.</a> J Neurosci. 36 (41), 10529-10544 (2016).</li></ol>

Voksende beviser har vist løftet om stamceller som en roman slagtilfælde terapi. Dette løfte har resulteret i en større indsats for at fremme stem cell therapeutics til sengen med mindst 40 igangværende eller afsluttede kliniske forsøg. Slagtilfælde patologi tilbyder en unik neurologisk lidelse, som egner sig til stamcelleterapi, fordi efter den akutte fornærmelse, der ikke er nogen neurodegenerative proces at forhindre inddrivelse. Den nøjagtige mekanisme af stamceller-forstærket slagtilfælde recovery er fort...

Slagtilfælde er den næststørste årsag til dødsfald i verden og den femte mest almindelige dødsårsag i USA. Trods disse høj dødelighed fortsat behandlinger for slagtilfælde recovery i øjeblikket en udfordring med ingen levedygtig medicinske muligheder i øjeblikket tilgængelige1. Der er i øjeblikket omkring 300 kliniske forsøg beskæftiger sig med iskæmisk slagtilfælde, hvoraf kun 40 udnytte stamceller. Tidligere undersøgelser har vist, at stamceller behandlingsformer har en gavnlig effekt på slagtilfælde reparation2,3. Paracrine faktorer som hjerne-afledte neurotrope faktor (BDNF) og thrombospondin-1 (THBS-1) frigivet fra transplanterede menneskelige neurale stamceller (hNPCs) har vist forbedret funktionel genopretning gennem mekanismer forbundet med en stigning i synapse dannelse, angiogenese, dendritiske forgrening og nye cytoskeletale fremskrivninger, samt modulere immunsystemet4,5,6. Men de optimale leveringsmetoder af stamcellerne fortsat undvigende.
Vellykket stamcelle levering til hjernen er fortsat en udfordring. I øjeblikket, er injicerbare hydrogel og polymere stilladser systemer indført for at levere stamceller. Disse leveringsmetoder beskytte stamceller under transplantation samt tilbyde beskyttelse mod barske efter slagtilfælde miljøet herunder værtens inflammatorisk respons og hypoksiske betingelser7,8,9 , 10. men de hyppigst anvendte materialer er inert, hvilket begrænser brugen af kontinuerlig graduering (dvs., elektrisk stimulation) af celler11. Elektrisk stimulation er en cue, der påvirker differentiering, ion-kanal tæthed og neurite udvækst af stamceller12. I forhold til inert polymerer, kan ledende polymerer bære en nuværende giver mulighed for elektrisk stimulation og manipulation af stamceller2. Men den præcise mekanisme som elektrisk stimulation modulerer neurotrope faktor release (dvs. BDNF og THBS-1) er stadig ikke fuldt undersøgt.
I denne protokol beskrive vi trin for at konstruere en celle kultur system bestående af en ledende polymer stillads, polypyrrole (PPy), der giver mulighed for in vitro- elektrisk stimulation. På grund af den måde, hvorpå celle kultur-systemet er fremstillet, er efterfølgende implantation af stamceller-seedede skafottet på peri-infarkt cortex muligt. For dette system forudsætning vi elektrisk stamceller på skafottet for en kort tidsperiode forud for implantation. Efter elektrisk stimulation, er den ledende polymer stillads transporterer cellerne med succes implanteret intracranially ved hjælp af en minimalt invasiv metode.

<table border="0" cellpadding="0" cellspacing="0" width="623">
	<tbody>
		<tr height="40">
			<td height="40" style="height:40px;width:213px;">FGF-Basic</td>
			<td style="width:103px;">Invitrogen</td>
			<td style="width:119px;">CTP0261</td>
			<td style="width:188px;">20 ng/mL for working media</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:213px;">Matrigel</td>
			<td style="width:103px;">Corning</td>
			<td style="width:119px;">cb40234a</td>
			<td style="width:188px;">1:200 dilution</td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:213px;">LIF Protein, Recom. Hum. (10 &#181;g/mL)</td>
			<td style="width:103px;">EMD Millipore</td>
			<td style="width:119px;">LIF1010</td>
			<td style="width:188px;">10 ng/mL for working media</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:213px;">Sylgard 184 silicone 3.9 kg</td>
			<td style="width:103px;">Fisherbrand</td>
			<td style="width:119px;">NC0162601</td>
			<td style="width:188px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:213px;">Hydrochloric acid</td>
			<td style="width:103px;">Fisherbrand</td>
			<td style="width:119px;">SA56-4</td>
			<td style="width:188px;"></td>
		</tr>
		<tr height="60">
			<td height="60" style="height:60px;width:213px;">Nitric Acid Concentrate (Certified) ACS, Fisher Chemical</td>
			<td style="width:103px;">Fisherbrand</td>
			<td style="width:119px;">SA95</td>
			<td style="width:188px;"></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:213px;">ITO Glass</td>
			<td style="width:103px;">Delta Technologies</td>
			<td style="width:119px;">CG-40IN-0115</td>
			<td style="width:188px;"></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:213px;">Sodium dodecylbenzenesulfonate</td>
			<td style="width:103px;">Sigma</td>
			<td style="width:119px;">289957-1KG</td>
			<td style="width:188px;"></td>
		</tr>
		<tr height="60">
			<td height="60" style="height:60px;width:213px;">Pyrrole</td>
			<td style="width:103px;">Sigma</td>
			<td style="width:119px;">131709-500ML</td>
			<td style="width:188px;">Protect pyrrole solution from light and room temperature</td>
		</tr>
		<tr height="60">
			<td height="60" style="height:60px;width:213px;">8 well glass slide chambers</td>
			<td style="width:103px;">Thermo Sci Nuc&#160;</td>
			<td style="width:119px;">125658</td>
			<td style="width:188px;">Detach the cell chamber and keep it under sterilized conditions</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:213px;">Flat-Surface Bracket, 3&#34;x1&#34;</td>
			<td style="width:103px;">McMaster-Carr&#160;</td>
			<td style="width:119px;">1030A4</td>
			<td style="width:188px;"></td>
		</tr>
		<tr height="60">
			<td height="60" style="height:60px;width:213px;">TWO PART SILVER PAINT 14G</td>
			<td style="width:103px;">Electron Microscopy Sciences&#160;</td>
			<td style="width:119px;">1264214</td>
			<td style="width:188px;">Mix two parts (1:1) in plastic plate</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:213px;">DPBS, 1x, with Ca and Mg, No Phenol Red</td>
			<td style="width:103px;">Genesse&#160;</td>
			<td style="width:119px;">25-508C</td>
			<td style="width:188px;"></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:213px;">AB2 ArunA Neural Cell Culture Media Kit</td>
			<td style="width:103px;">Aruna Biomedical&#160;</td>
			<td style="width:119px;">ABNS7013.2</td>
			<td style="width:188px;"></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:213px;">hNP1 Human Neural Progenitor Expansion Kit</td>
			<td style="width:103px;">Aruna Biomedical&#160;</td>
			<td style="width:119px;">&#160;hNP7013.1</td>
			<td style="width:188px;"></td>
		</tr>
		<tr height="60">
			<td height="60" style="height:60px;width:213px;">Noncontact Flow-Adjustment Valve, Nickel-Plated Brass, for 3/32&#34; to 5/8&#34; Tube OD</td>
			<td style="width:103px;">McMaster-Carr&#160;</td>
			<td style="width:119px;">5330K22</td>
			<td style="width:188px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:213px;">Multimeter</td>
			<td style="width:103px;">Keysight&#160;</td>
			<td style="width:119px;">E3641A</td>
			<td style="width:188px;"></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:213px;">Wavefoam generator</td>
			<td style="width:103px;">Keysight</td>
			<td style="width:119px;">33210A-10MHz</td>
			<td style="width:188px;"></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:213px;">Pt meshes</td>
			<td style="width:103px;">Sigma-Aldrich&#160;</td>
			<td style="width:119px;">298107-425MG</td>
			<td style="width:188px;">Reference electrode with dimensions, 1x1 cm</td>
		</tr>
		<tr height="61">
			<td height="61" style="height:61px;width:213px;">LIVE/DEAD Viability/Cytotoxicity Kit, for mammalian cells</td>
			<td style="width:103px;">Thermo Fisher Scientific&#160;</td>
			<td style="width:119px;">L3224</td>
			<td></td>
		</tr>
		<tr height="61">
			<td height="61" style="height:61px;width:213px;">BDNF</td>
			<td style="width:103px;">Thermo Fisher Scientific&#160;</td>
			<td style="width:119px;">Hs02718934_s1</td>
			<td style="width:188px;"></td>
		</tr>
		<tr height="61">
			<td height="61" style="height:61px;">THBS1</td>
			<td style="width:103px;">Thermo Fisher Scientific&#160;</td>
			<td style="width:119px;">Hs00962908_m1</td>
			<td></td>
		</tr>
		<tr height="61">
			<td height="61" style="height:61px;width:213px;">GAPDH</td>
			<td style="width:103px;">Thermo Fisher Scientific&#160;</td>
			<td style="width:119px;">Hs02758991_g1</td>
			<td style="width:188px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:213px;">RNeasy Mini Kit (250)</td>
			<td style="width:103px;">Qiagen</td>
			<td style="width:119px;">74106</td>
			<td style="width:188px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:213px;">QIAshredder (250)</td>
			<td style="width:103px;">Qiagen</td>
			<td style="width:119px;">79656</td>
			<td style="width:188px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:213px;">RNase-Free DNase Set (50)</td>
			<td style="width:103px;">QIAGEN</td>
			<td style="width:119px;">79254</td>
			<td style="width:188px;"></td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:213px;">iScript cDNA Synthesis Kit, 100 x 20 &#181;L rxns</td>
			<td style="width:103px;">BIORAD</td>
			<td style="width:119px;">1708891</td>
			<td style="width:188px;"></td>
		</tr>
		<tr height="61">
			<td height="61" style="height:61px;width:213px;">TaqMan Gene Expression Master Mix</td>
			<td style="width:103px;">Thermo Fisher Scientific&#160;</td>
			<td style="width:119px;">4369510</td>
			<td></td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:213px;">7-8 Week Old, male, RNU Rats</td>
			<td style="width:103px;">NCI-Frederick</td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:213px;">4-0 Ethicon Silk Suture</td>
			<td style="width:103px;">eSutures.com</td>
			<td style="width:119px;">683G</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:213px;">Isoflurane</td>
			<td style="width:103px;">Henry Schein</td>
			<td style="width:119px;">29405</td>
			<td></td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:213px;">V-1 Tabletop Laboratory Animal Anesthesia System</td>
			<td style="width:103px;">VetEquip</td>
			<td style="width:119px;">901806</td>
			<td></td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:213px;">Surgicel Original Absorbable Hemostat</td>
			<td style="width:103px;">Ethicon</td>
			<td style="width:119px;">1952</td>
			<td></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:213px;">Lab Standard Stereotaxic Instrument, Rat</td>
			<td style="width:103px;">Stoelting</td>
			<td style="width:119px;">51600</td>
			<td></td>
		</tr>
		<tr height="80">
			<td height="80" style="height:80px;width:213px;">Kimberly-Clark Professional&#160;Safeskin Purple Nitrile Sterile Exam Gloves</td>
			<td style="width:103px;">Fisherbrand</td>
			<td style="width:119px;">19-063-130</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:213px;">Sterile Drape</td>
			<td style="width:103px;">Medline</td>
			<td style="width:119px;">DYNJSD1092</td>
			<td></td>
		</tr>
		<tr height="80">
			<td height="80" style="height:80px;width:213px;">Thermo Scientific&#160;Shandon Stainless-Steel Scalpel Blade Handle, Holds No. 20-25 Blades</td>
			<td style="width:103px;">Fisherbrand</td>
			<td style="width:119px;">53-34</td>
			<td></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:213px;">Walter Stern Scalpel Blade Series 300</td>
			<td style="width:103px;">Fisherbrand</td>
			<td>17-654-456</td>
			<td></td>
		</tr>
		<tr height="61">
			<td height="61" style="height:61px;width:213px;">QuantStudio 6 Flex Real-Time PCR System</td>
			<td style="width:103px;">Thermo Fisher Scientific</td>
			<td style="width:119px;">4484642</td>
			<td></td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:213px;">Frazier Micro Dissecting Hook</td>
			<td style="width:103px;">Harvard Apparatus</td>
			<td>52-2706</td>
			<td></td>
		</tr>
	</tbody>
</table>

electrically conductive scaffold to modulate and deliver stem cells

Stamcelleterapi har vist sig som en spændende slagtilfælde terapeutisk, men den optimale leveringsmetode er fortsat uklart. Mens teknikken med mikroinjektion har været anvendt i årtier for at levere stamceller i slagtilfælde modeller, er denne teknik begrænset af manglen evne til at manipulere stamceller inden injektionen. Dette papir beskriver en metode til at bruge en elektrisk ledende polymer stillads til stamceller levering. Elektrisk stimulation af stamceller ved hjælp af en ledende polymer stillads ændrer stamcelles gener involveret i celle overlevelse, inflammatorisk respons og synaptisk remodellering. Efter elektrisk forkonditionering, kan stamceller på skafottet transplanteres intracranially i distale midt cerebral arterie okklusion rotte model. Denne protokol beskriver en kraftfuld teknik til at manipulere stamceller via en ledende polymer stillads og skaber et nyt værktøj til at videreudvikle stamcelle-baseret terapi.

Den skematiske vist i figur 1 repræsenterer den samlede arbejdsgang af den elektriske stimulation af hNPCs og potentielle downstream applikationer. En strømbegrænsning i stamcelleterapi er, at stamceller er udsat for en hård efter transplantation miljø herunder betændelse og iskæmisk betingelser. Disse vanskelige forhold sandsynligvis begrænse deres terapeutiske virkning14,15. Brug af en leden...

Watch this Scientific Journal Video about Electrically Conductive Scaffold to Modulate and Deliver Stem Cells at JoVE.com

Electrically Conductive Scaffold to Modulate and Deliver Stem Cells

Denne protokol beskriver fabrikation af en celle kultur system til at tillade såning af stamceller på en ledende polymer stillads til in vitro- elektrisk stimulation og efterfølgende i vivo implantation af stamceller-seedede stillads ved hjælp af en minimalt invasive teknik.

Elektrisk ledende stillads til at modulere og levere stamceller

Stem cell therapy has emerged as an exciting stroke therapeutic, but the optimal delivery method remains unclear. While the technique of microinjection ...

Research

JoVE Journal

Bioengineering

Use of Human Perivascular Stem Cells for Bone Regeneration

Brug af menneskelige perivaskulær stamceller til Bone Regeneration

Human perivascular stem cells (PSCs) can be isolated in sufficient numbers from multiple tissues for purposes of skeletal tissue engineering1-3. PSCs are ...

Planar and Three-Dimensional Printing of Conductive Inks

Planar-og tredimensionelle Udskrivning af ledende blæk

Printed electronics rely on low-cost, large-area fabrication routes to create flexible or multidimensional electronic, optoelectronic, and biomedical ...

Design of a Cyclic Pressure Bioreactor for the Ex Vivo Study of Aortic Heart Valves

Design of a Cyclic Pressure Bioreactor for the Ex Vivo Study of Aortic Heart Valves

The aortic valve, located between the left ventricle and the aorta, allows for unidirectional blood flow, preventing backflow into the ventricle. Aortic ...

Alginate Microcapsule as a 3D Platform for Propagation and Differentiation of Human Embryonic Stem Cells (hESC) to Different Lineages

Alginate Microcapsule as a 3D Platform for Propagation and Differentiation of Human Embryonic Stem Cells hESC to Different Lineages

Alginat mikrokapselsammensætning som en 3D-platform for Formering og differentiering af humane embryonale stamceller (embryonale) til forskellige linier

Human embryonic stem cells (hESC) are emerging as an attractive alternative source for cell replacement therapy since they can be expanded in culture ...

Development, Expansion, and In vivo Monitoring of Human NK Cells from Human Embryonic Stem Cells (hESCs) and Induced Pluripotent Stem Cells (iPSCs)

Development, Expansion, and In vivo Monitoring of Human NK Cells from Human Embryonic Stem Cells hESCs and Induced Pluripotent Stem Cells iPSCs

Udvikling, Expansion, og<em&gt; In vivo</em&gt; Overvågning af menneskerettigheder NK-celler fra humane embryonale stamceller (hESCs), og og induceret pluripotente stamceller (iPSCs)

We present a method for deriving natural killer (NK) cells from undifferentiated hESCs and iPSCs using a feeder-free approach. This method gives rise to ...

Transplantation of Induced Pluripotent Stem Cell-derived Mesoangioblast-like Myogenic Progenitors in Mouse Models of Muscle Regeneration

Transplantation af induceret pluripotente stamcelle-afledt Mesoangioblast-lignende Myogenic Stamfadere i Mouse Modeller af muskel regenerering

Patient-derived iPSCs could be an invaluable source of cells for future autologous cell therapy protocols. iPSC-derived myogenic stem/progenitor cells ...

Ordering Single Cells and Single Embryos in 3D Confinement: A New Device for High Content Screening

Bestilling enkelte celler og Single Embryoner i 3D Indespærring: En ny enhed for High Content Screening

Biological cells are usually observed on flat (2D) surfaces. This condition is not physiological, and phenotypes and shapes are highly variable. Screening ...

Microdissection of Primary Renal Tissue Segments and Incorporation with Novel Scaffold-free Construct Technology

Microdissection af primær Renal væv segmenter og iblandingen med romanen stillads-fri konstruktion teknologi

Kidney transplantation is now a mainstream therapy for end-stage renal disease. However, with approximately 96,000 people on the waiting list and only ...

Protocol for MicroRNA Transfer into Adult Bone Marrow-derived Hematopoietic Stem Cells to Enable Cell Engineering Combined with Magnetic Targeting

Protokol for mikroRNA overførsel i knoglemarv-afledte hæmatopoietisk stamceller til at aktivere celle Engineering kombineret med magnetisk målretning

While CD133+ hematopoietic stem cells (SCs) have been proven to provide high potential in the field of regenerative medicine, their low retention rates ...

Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Single-Cell Optisk Action Potentiel måling i human induceret pluripotente stamcelle-afledte kardiomyocytter

Conventional intracellular microelectrode techniques to quantify cardiomyocyte electrophysiology are extremely complex, labor intensive, and typically ...