Name: electrically conductive scaffold to modulate and deliver stem cells
Uploaded: 2018-04-13T13:30:00
Description: Watch this Scientific Journal Video about Electrically Conductive Scaffold to Modulate and Deliver Stem Cells at JoVE.com

Ringraziamo il dottor Kati Andreasson (dipartimento di neurologia e scienze neurologiche, Università di Stanford) per l'uso della macchina qRT-PCR. Il lavoro è stato supportato da nazionale istituti di salute sovvenzioni K08NS098876 (alla P.M.G) e Postdoctoral Fellowship di Stanford School of Medicine Dean (a B.O.).

Tutte le cellule staminali e procedure degli animali sono state approvate dal comitato di supervisione di ricerca sulle cellule staminali di Stanford e dal pannello di amministrazione dell'Università di Stanford su Laboratory Animal Care (SCRO-616 e APLAC-31909).
1. incisione del vetro di ITO
<ol><li>Preparare l'ossido della latta dell'indio (ITO)-vetrino coperto inserendo un agente mascherante sopra il lato conduttivo del vetro. Nota: Nastri trasparenti più commerciali possono essere u...

<ol>
	<li>Moskowitz, M. A., Lo, E. H., Iadecola, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Science+of+Stroke:+Mechanisms+in+Search+of+Treatments.">The Science of Stroke: Mechanisms in Search of Treatments.</a> Neuron. 67 (2), 181-198 (2010).</li><li>George, P. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Electrical+Preconditioning+of+Stem+Cells+with+a+Conductive+Polymer+Scaffold+Enhances+Stroke+Recovery.">Electrical Preconditioning of Stem Cells with a Conductive Polymer Scaffold Enhances Stroke Recovery.</a> Biomaterials. 142, 31-40 (2017).</li><li>Steinberg, G. K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Clinical+Outcomes+of+Transplanted+Modified+Bone+Marrow-Derived+Mesenchymal+Stem+Cells+in+Stroke:+A+Phase+1/2a+Study.">Clinical Outcomes of Transplanted Modified Bone Marrow-Derived Mesenchymal Stem Cells in Stroke: A Phase 1/2a Study.</a> Stroke. 47 (7), 1817-1824 (2016).</li><li>George, P. M., Steinberg, G. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Novel+Stroke+Therapeutics:+Unraveling+Stroke+Pathophysiology+and+Its+Impact+on+Clinical+Treatments.">Novel Stroke Therapeutics: Unraveling Stroke Pathophysiology and Its Impact on Clinical Treatments.</a> Neuron. 87 (2), 297-309 (2015).</li><li>Schabitz, W. R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intravenous+Brain-Derived+Neurotrophic+Factor+Enhances+Poststroke+Sensorimotor+Recovery+and+Stimulates+Neurogenesis.">Intravenous Brain-Derived Neurotrophic Factor Enhances Poststroke Sensorimotor Recovery and Stimulates Neurogenesis.</a> Stroke. 38 (7), 2165-2172 (2007).</li><li>Liauw, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Thrombospondins+1+and+2+Are+Necessary+For+Synaptic+Plasticity+and+Functional+Recovery+After+Stroke.">Thrombospondins 1 and 2 Are Necessary For Synaptic Plasticity and Functional Recovery After Stroke.</a> J Cereb Blood Flow Metab. 28 (10), 1722-1732 (2008).</li><li>Cantu, D. A., Hematti, P., Kao, W. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cell+Encapsulating+Biomaterial+Regulates+Mesenchymal+Stromal/Stem+Cell+Differentiation+and+Macrophage+Immunophenotype.">Cell Encapsulating Biomaterial Regulates Mesenchymal Stromal/Stem Cell Differentiation and Macrophage Immunophenotype.</a> Stem Cells Translational Medicine. 1 (10), 740-749 (2012).</li><li>Chaudhuri, O., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hydrogels+with+Tunable+Stress+Relaxation+Regulate+Stem+Cell+Fate+and+Activity.">Hydrogels with Tunable Stress Relaxation Regulate Stem Cell Fate and Activity.</a> Nat Mater. 15 (3), 326-334 (2016).</li><li>Ballios, B. G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+Hyaluronan-Based+Injectable+Hydrogel+Improves+the+Survival+and+Integration+of+Stem+Cell+Progeny+following+Transplantation.">A Hyaluronan-Based Injectable Hydrogel Improves the Survival and Integration of Stem Cell Progeny following Transplantation.</a> Stem Cell Reports. 4 (6), 1031-1045 (2015).</li><li>Mothe, A. J., Tam, R. Y., Zahir, T., Tator, C. H., Shoichet, M. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Repair+of+the+Injured+Spinal+Cord+by+Transplantation+of+Neural+Stem+Cells+in+a+hyaluronan-based+hydrogel.">Repair of the Injured Spinal Cord by Transplantation of Neural Stem Cells in a hyaluronan-based hydrogel.</a> Biomaterials. 34 (15), 3775-3783 (2013).</li><li>Saini, M., Singh, Y., Arora, P., Arora, V., Jain, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Implant+Biomaterials:+A+Comprehensive+Review.">Implant Biomaterials: A Comprehensive Review.</a> World J Clin Cases. 3 (1), 52-57 (2015).</li><li>Huang, Y., Li, Y., Chen, J., Zhou, H., Tan, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Electrical+Stimulation+Elicits+Neural+Stem+Cells+Activation:+New+Perspectives+in+CNS+Repair.">Electrical Stimulation Elicits Neural Stem Cells Activation: New Perspectives in CNS Repair.</a> Front Hum Neurosci. 9, (2015).</li><li>Pritchett-Corning, K. R., Mulder, G. B., Luo, Y., White, W. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Principles+of+Rodent+Surgery+for+the+New+Surgeon.">Principles of Rodent Surgery for the New Surgeon.</a> J Vis Exp. (47), (2011).</li><li>Abdelwahid, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Stem+Cell+Death+and+Survival+in+Heart+Regeneration+and+Repair.">Stem Cell Death and Survival in Heart Regeneration and Repair.</a> Apoptosis. 21 (3), 252-268 (2016).</li><li>Wang, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Clinical+Status+of+Stem+Cell+Therapy+for+Ischemic+Cardiomyopathy.">The Clinical Status of Stem Cell Therapy for Ischemic Cardiomyopathy.</a> Stem Cells Int. 2015, (2015).</li><li>George, P. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fabrication+and+Biocompatibility+of+Polypyrrole+Implants+Suitable+for+Neural+Prosthetics.">Fabrication and Biocompatibility of Polypyrrole Implants Suitable for Neural Prosthetics.</a> Biomaterials. 26 (17), 3511-3519 (2005).</li><li>Aravamudan, B., Thompson, M., Pabelick, C., Prakash, Y. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Brain-Derived+Neurotrophic+Factor+Induces+Proliferation+of+Human+Airway+Smooth+Muscle+Cells.">Brain-Derived Neurotrophic Factor Induces Proliferation of Human Airway Smooth Muscle Cells.</a> J Cell Mol Med. 16 (4), 812-823 (2012).</li><li>Lopes, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Thrombospondin+2+Regulates+Cell+Proliferation+Induced+by+Rac1+Redox-Dependent+Signaling.">Thrombospondin 2 Regulates Cell Proliferation Induced by Rac1 Redox-Dependent Signaling.</a> Mol Cell Biol. 23 (15), 5401-5408 (2003).</li><li>Ploughman, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Brain-Derived+Neurotrophic+Factor+Contributes+to+Recovery+of+Skilled+Reaching+After+Focal+Ischemia+in+Rats.">Brain-Derived Neurotrophic Factor Contributes to Recovery of Skilled Reaching After Focal Ischemia in Rats.</a> Stroke. 40 (4), 1490-1495 (2009).</li><li>Baker, E. W., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Induced+Pluripotent+Stem+Cell-Derived+Neural+Stem+Cell+Therapy+Enhances+Recovery+in+an+Ischemic+Stroke+Pig+Model.">Induced Pluripotent Stem Cell-Derived Neural Stem Cell Therapy Enhances Recovery in an Ischemic Stroke Pig Model.</a> Sci Rep. 7 (1), 10075 (2017).</li><li>Bacigaluppi, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neural+Stem+Cell+Transplantation+Induces+Stroke+Recovery+by+Upregulating+Glutamate+Transporter+GLT-1+in+Astrocytes.">Neural Stem Cell Transplantation Induces Stroke Recovery by Upregulating Glutamate Transporter GLT-1 in Astrocytes.</a> J Neurosci. 36 (41), 10529-10544 (2016).</li></ol>

Crescenti evidenze ha dimostrato la promessa delle cellule staminali come terapia romanzo tratto. Questa promessa ha provocato un grande sforzo per avanzare terapeutica delle cellule staminali al capezzale con almeno 40 studi clinici in corso o completati. Patologia di corsa offre un unico disturbo neurologico che si presta a terapia con cellule staminali, perché dopo l'insulto acuto, non c'è nessun processo neurodegenerativo, impedendo così il recupero. Il meccanismo esatto di cellule staminali-aumentata tempi di rec...

Il colpo è la seconda causa di morte nel mondo e la quinta causa di morte negli Stati Uniti. Nonostante questi alti tassi di mortalità, trattamenti per tempi di recupero attualmente rimangono una sfida con nessun valide opzioni mediche attualmente disponibili1. Ci sono attualmente circa 300 test clinici a che fare con colpi ischemici, di cui solo 40 utilizzare cellule staminali. Gli studi precedenti hanno dimostrato che le terapie con cellule staminali hanno un effetto benefico sulla corsa riparazione2,3. Fattori paracrini come fattore neurotrophic cervello-derivato (BDNF) e trombospondina-1 (THBS-1) rilasciati dalle cellule progenitrici neurali umane trapiantate (hNPCs) hanno mostrato una migliore recupero funzionale attraverso meccanismi associati con un aumento Synapse formazione, angiogenesi, ramificazioni dendritiche e nuove proiezioni assonali, come pure di modulazione del sistema immunitario4,5,6. Tuttavia, i metodi di consegna ottimale delle cellule staminali rimangono sfuggenti.
Consegna di successo sulle cellule staminali al cervello rimane una sfida. Attualmente, idrogel iniettabili e sistemi di ponteggio polimerici sono stati introdotti per fornire cellule staminali. Questi metodi di consegna proteggono le cellule staminali durante il trapianto, nonché offrono protezione da ambienti post-ictus tra cui risposta infiammatoria dell'ospite e condizioni ipossiche7,8,9 , 10. Tuttavia, i materiali più comunemente usati sono inerti, che limita l'uso della modulazione continua (cioè, stimolazione elettrica) delle cellule11. La stimolazione elettrica è uno spunto che influenza la differenziazione, densità di canali ionici e neuriti delle cellule staminali12. Rispetto ai polimeri inerti, polimeri conduttivi possono trasportare una corrente che consente per la stimolazione elettrica e la manipolazione di cellule staminali2. Tuttavia, il meccanismo preciso con cui stimolazione elettrica modula il rilascio di fattore neurotrophic (cioè, BDNF e THBS-1) è ancora non completamente esplorato.
In questo protocollo, descriviamo i passaggi per costruire un sistema di coltura cellulare che consiste di uno scaffold polimerici, polipirrolo (PPy), che permette per la stimolazione elettrica in vitro . A causa del modo in cui è realizzato il sistema di coltura cellulare, è possibile impianto successivo dell'impalcatura della cellula formativa-seminato sulla corteccia del peri-infarto. Per questo sistema, abbiamo presupposto elettricamente le cellule staminali sul patibolo per un breve periodo di tempo prima dell'impianto. A seguito di stimolazione elettrica, lo scaffold polimerici che trasportano le cellule è impiantato con successo intracranially utilizzando un metodo come minimo dilagante.

<table border="0" cellpadding="0" cellspacing="0" width="623">
	<tbody>
		<tr height="40">
			<td height="40" style="height:40px;width:213px;">FGF-Basic</td>
			<td style="width:103px;">Invitrogen</td>
			<td style="width:119px;">CTP0261</td>
			<td style="width:188px;">20 ng/mL for working media</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:213px;">Matrigel</td>
			<td style="width:103px;">Corning</td>
			<td style="width:119px;">cb40234a</td>
			<td style="width:188px;">1:200 dilution</td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:213px;">LIF Protein, Recom. Hum. (10 &#181;g/mL)</td>
			<td style="width:103px;">EMD Millipore</td>
			<td style="width:119px;">LIF1010</td>
			<td style="width:188px;">10 ng/mL for working media</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:213px;">Sylgard 184 silicone 3.9 kg</td>
			<td style="width:103px;">Fisherbrand</td>
			<td style="width:119px;">NC0162601</td>
			<td style="width:188px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:213px;">Hydrochloric acid</td>
			<td style="width:103px;">Fisherbrand</td>
			<td style="width:119px;">SA56-4</td>
			<td style="width:188px;"></td>
		</tr>
		<tr height="60">
			<td height="60" style="height:60px;width:213px;">Nitric Acid Concentrate (Certified) ACS, Fisher Chemical</td>
			<td style="width:103px;">Fisherbrand</td>
			<td style="width:119px;">SA95</td>
			<td style="width:188px;"></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:213px;">ITO Glass</td>
			<td style="width:103px;">Delta Technologies</td>
			<td style="width:119px;">CG-40IN-0115</td>
			<td style="width:188px;"></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:213px;">Sodium dodecylbenzenesulfonate</td>
			<td style="width:103px;">Sigma</td>
			<td style="width:119px;">289957-1KG</td>
			<td style="width:188px;"></td>
		</tr>
		<tr height="60">
			<td height="60" style="height:60px;width:213px;">Pyrrole</td>
			<td style="width:103px;">Sigma</td>
			<td style="width:119px;">131709-500ML</td>
			<td style="width:188px;">Protect pyrrole solution from light and room temperature</td>
		</tr>
		<tr height="60">
			<td height="60" style="height:60px;width:213px;">8 well glass slide chambers</td>
			<td style="width:103px;">Thermo Sci Nuc&#160;</td>
			<td style="width:119px;">125658</td>
			<td style="width:188px;">Detach the cell chamber and keep it under sterilized conditions</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:213px;">Flat-Surface Bracket, 3&#34;x1&#34;</td>
			<td style="width:103px;">McMaster-Carr&#160;</td>
			<td style="width:119px;">1030A4</td>
			<td style="width:188px;"></td>
		</tr>
		<tr height="60">
			<td height="60" style="height:60px;width:213px;">TWO PART SILVER PAINT 14G</td>
			<td style="width:103px;">Electron Microscopy Sciences&#160;</td>
			<td style="width:119px;">1264214</td>
			<td style="width:188px;">Mix two parts (1:1) in plastic plate</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:213px;">DPBS, 1x, with Ca and Mg, No Phenol Red</td>
			<td style="width:103px;">Genesse&#160;</td>
			<td style="width:119px;">25-508C</td>
			<td style="width:188px;"></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:213px;">AB2 ArunA Neural Cell Culture Media Kit</td>
			<td style="width:103px;">Aruna Biomedical&#160;</td>
			<td style="width:119px;">ABNS7013.2</td>
			<td style="width:188px;"></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:213px;">hNP1 Human Neural Progenitor Expansion Kit</td>
			<td style="width:103px;">Aruna Biomedical&#160;</td>
			<td style="width:119px;">&#160;hNP7013.1</td>
			<td style="width:188px;"></td>
		</tr>
		<tr height="60">
			<td height="60" style="height:60px;width:213px;">Noncontact Flow-Adjustment Valve, Nickel-Plated Brass, for 3/32&#34; to 5/8&#34; Tube OD</td>
			<td style="width:103px;">McMaster-Carr&#160;</td>
			<td style="width:119px;">5330K22</td>
			<td style="width:188px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:213px;">Multimeter</td>
			<td style="width:103px;">Keysight&#160;</td>
			<td style="width:119px;">E3641A</td>
			<td style="width:188px;"></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:213px;">Wavefoam generator</td>
			<td style="width:103px;">Keysight</td>
			<td style="width:119px;">33210A-10MHz</td>
			<td style="width:188px;"></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:213px;">Pt meshes</td>
			<td style="width:103px;">Sigma-Aldrich&#160;</td>
			<td style="width:119px;">298107-425MG</td>
			<td style="width:188px;">Reference electrode with dimensions, 1x1 cm</td>
		</tr>
		<tr height="61">
			<td height="61" style="height:61px;width:213px;">LIVE/DEAD Viability/Cytotoxicity Kit, for mammalian cells</td>
			<td style="width:103px;">Thermo Fisher Scientific&#160;</td>
			<td style="width:119px;">L3224</td>
			<td></td>
		</tr>
		<tr height="61">
			<td height="61" style="height:61px;width:213px;">BDNF</td>
			<td style="width:103px;">Thermo Fisher Scientific&#160;</td>
			<td style="width:119px;">Hs02718934_s1</td>
			<td style="width:188px;"></td>
		</tr>
		<tr height="61">
			<td height="61" style="height:61px;">THBS1</td>
			<td style="width:103px;">Thermo Fisher Scientific&#160;</td>
			<td style="width:119px;">Hs00962908_m1</td>
			<td></td>
		</tr>
		<tr height="61">
			<td height="61" style="height:61px;width:213px;">GAPDH</td>
			<td style="width:103px;">Thermo Fisher Scientific&#160;</td>
			<td style="width:119px;">Hs02758991_g1</td>
			<td style="width:188px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:213px;">RNeasy Mini Kit (250)</td>
			<td style="width:103px;">Qiagen</td>
			<td style="width:119px;">74106</td>
			<td style="width:188px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:213px;">QIAshredder (250)</td>
			<td style="width:103px;">Qiagen</td>
			<td style="width:119px;">79656</td>
			<td style="width:188px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:213px;">RNase-Free DNase Set (50)</td>
			<td style="width:103px;">QIAGEN</td>
			<td style="width:119px;">79254</td>
			<td style="width:188px;"></td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:213px;">iScript cDNA Synthesis Kit, 100 x 20 &#181;L rxns</td>
			<td style="width:103px;">BIORAD</td>
			<td style="width:119px;">1708891</td>
			<td style="width:188px;"></td>
		</tr>
		<tr height="61">
			<td height="61" style="height:61px;width:213px;">TaqMan Gene Expression Master Mix</td>
			<td style="width:103px;">Thermo Fisher Scientific&#160;</td>
			<td style="width:119px;">4369510</td>
			<td></td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:213px;">7-8 Week Old, male, RNU Rats</td>
			<td style="width:103px;">NCI-Frederick</td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:213px;">4-0 Ethicon Silk Suture</td>
			<td style="width:103px;">eSutures.com</td>
			<td style="width:119px;">683G</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:213px;">Isoflurane</td>
			<td style="width:103px;">Henry Schein</td>
			<td style="width:119px;">29405</td>
			<td></td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:213px;">V-1 Tabletop Laboratory Animal Anesthesia System</td>
			<td style="width:103px;">VetEquip</td>
			<td style="width:119px;">901806</td>
			<td></td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:213px;">Surgicel Original Absorbable Hemostat</td>
			<td style="width:103px;">Ethicon</td>
			<td style="width:119px;">1952</td>
			<td></td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:213px;">Lab Standard Stereotaxic Instrument, Rat</td>
			<td style="width:103px;">Stoelting</td>
			<td style="width:119px;">51600</td>
			<td></td>
		</tr>
		<tr height="80">
			<td height="80" style="height:80px;width:213px;">Kimberly-Clark Professional&#160;Safeskin Purple Nitrile Sterile Exam Gloves</td>
			<td style="width:103px;">Fisherbrand</td>
			<td style="width:119px;">19-063-130</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:213px;">Sterile Drape</td>
			<td style="width:103px;">Medline</td>
			<td style="width:119px;">DYNJSD1092</td>
			<td></td>
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			<td height="80" style="height:80px;width:213px;">Thermo Scientific&#160;Shandon Stainless-Steel Scalpel Blade Handle, Holds No. 20-25 Blades</td>
			<td style="width:103px;">Fisherbrand</td>
			<td style="width:119px;">53-34</td>
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			<td height="40" style="height:40px;width:213px;">Walter Stern Scalpel Blade Series 300</td>
			<td style="width:103px;">Fisherbrand</td>
			<td>17-654-456</td>
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			<td height="61" style="height:61px;width:213px;">QuantStudio 6 Flex Real-Time PCR System</td>
			<td style="width:103px;">Thermo Fisher Scientific</td>
			<td style="width:119px;">4484642</td>
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			<td height="41" style="height:41px;width:213px;">Frazier Micro Dissecting Hook</td>
			<td style="width:103px;">Harvard Apparatus</td>
			<td>52-2706</td>
			<td></td>
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	</tbody>
</table>

electrically conductive scaffold to modulate and deliver stem cells

Terapia con cellule staminali è emerso come un'eccitante corsa terapeutica, ma il metodo di consegna ottimale rimane poco chiaro. Mentre la tecnica di microiniezione è stata utilizzata per decenni per fornire cellule staminali in modelli di ictus, questa tecnica è limitata dalla mancanza di capacità di manipolare le cellule staminali prima dell'iniezione. Questa carta descrive in dettaglio un metodo di utilizzo di un'impalcatura polimero conduttivo per la consegna di cellule staminali. La stimolazione elettrica delle cellule staminali utilizzando un'impalcatura polimero conduttivo altera geni di cellule staminali coinvolti nella sopravvivenza delle cellule, la risposta infiammatoria e rimodellamento sinaptico. Dopo precondizionamento elettrici, le cellule staminali sul patibolo sono trapiantate intracranially in un modello del ratto di occlusione dell'arteria cerebrale centrale distale. Questo protocollo descrive una tecnica potente per manipolare le cellule staminali tramite un'impalcatura polimero conduttivo e crea un nuovo strumento per sviluppare ulteriormente la terapia a base di cellule staminali.

Lo schema illustrato nella Figura 1 rappresenta il flusso di lavoro complessivo della stimolazione elettrica dei hNPCs e potenziali applicazioni a valle. Una limitazione corrente nella terapia con cellule staminali è che le cellule staminali sono esposti a un ambiente di post-trapianto duro tra cui l'infiammazione e condizioni ischemiche. Queste difficili condizioni probabilmente limitano la loro efficacia terapeutica14,<su...

Watch this Scientific Journal Video about Electrically Conductive Scaffold to Modulate and Deliver Stem Cells at JoVE.com

Electrically Conductive Scaffold to Modulate and Deliver Stem Cells

Questo protocollo descrive la realizzazione di un sistema di coltura cellulare per consentire la semina delle cellule staminali su un'impalcatura polimero conduttivo per elettrostimolazione in vitro e successive in vivo l'impianto della cellula formativa-seminato impalcatura utilizzando un tecnica mininvasiva.

Elettricamente conduttivo impalcatura di modulare e fornire cellule staminali

Stem cell therapy has emerged as an exciting stroke therapeutic, but the optimal delivery method remains unclear. While the technique of microinjection ...

Research

JoVE Journal

Bioengineering

Use of Human Perivascular Stem Cells for Bone Regeneration

L&#39;uso di cellule staminali per la rigenerazione ossea perivascolari

Human perivascular stem cells (PSCs) can be isolated in sufficient numbers from multiple tissues for purposes of skeletal tissue engineering1-3. PSCs are ...

Ricerca

Bioingegneria

Planar and Three-Dimensional Printing of Conductive Inks

Stampa planare e tridimensionale di inchiostri conduttivi

Printed electronics rely on low-cost, large-area fabrication routes to create flexible or multidimensional electronic, optoelectronic, and biomedical ...

Design of a Cyclic Pressure Bioreactor for the Ex Vivo Study of Aortic Heart Valves

Design of a Cyclic Pressure Bioreactor for the Ex Vivo Study of Aortic Heart Valves

Progettazione di un bioreattore per la pressione ciclica Ex Vivo Studio delle valvole cardiache aortica

The aortic valve, located between the left ventricle and the aorta, allows for unidirectional blood flow, preventing backflow into the ventricle. Aortic ...

Alginate Microcapsule as a 3D Platform for Propagation and Differentiation of Human Embryonic Stem Cells (hESC) to Different Lineages

Alginate Microcapsule as a 3D Platform for Propagation and Differentiation of Human Embryonic Stem Cells hESC to Different Lineages

Microcapsule di alginato come piattaforma 3D per la propagazione e la differenziazione delle cellule staminali embrionali umane (hESC) per diverse linee

Human embryonic stem cells (hESC) are emerging as an attractive alternative source for cell replacement therapy since they can be expanded in culture ...

Development, Expansion, and In vivo Monitoring of Human NK Cells from Human Embryonic Stem Cells (hESCs) and Induced Pluripotent Stem Cells (iPSCs)

Development, Expansion, and In vivo Monitoring of Human NK Cells from Human Embryonic Stem Cells hESCs and Induced Pluripotent Stem Cells iPSCs

Sviluppo, di espansione, e<em&gt; In vivo</em&gt; Monitoraggio delle cellule NK umane da cellule staminali embrionali umane (hESC) ed e cellule staminali pluripotenti indotte (iPSCs)

We present a method for deriving natural killer (NK) cells from undifferentiated hESCs and iPSCs using a feeder-free approach. This method gives rise to ...

Transplantation of Induced Pluripotent Stem Cell-derived Mesoangioblast-like Myogenic Progenitors in Mouse Models of Muscle Regeneration

Trapianto di progenitori miogenici pluripotenti derivati da cellule staminali indotte simili a mesoangioblasti nei modelli di topo di rigenerazione muscolare

Patient-derived iPSCs could be an invaluable source of cells for future autologous cell therapy protocols. iPSC-derived myogenic stem/progenitor cells ...

Ordering Single Cells and Single Embryos in 3D Confinement: A New Device for High Content Screening

Ordinazione di singole cellule e singola embrioni in 3D confinamento: Un nuovo dispositivo per l&#39;alta Screening contenuti

Biological cells are usually observed on flat (2D) surfaces. This condition is not physiological, and phenotypes and shapes are highly variable. Screening ...

Microdissection of Primary Renal Tissue Segments and Incorporation with Novel Scaffold-free Construct Technology

Microdissection di segmenti di tessuto renale primario e incorporazione con tecnologia romanzo costrutto senza impalcatura

Kidney transplantation is now a mainstream therapy for end-stage renal disease. However, with approximately 96,000 people on the waiting list and only ...

Protocol for MicroRNA Transfer into Adult Bone Marrow-derived Hematopoietic Stem Cells to Enable Cell Engineering Combined with Magnetic Targeting

Protocollo per il trasferimento di MicroRNA in adulto cellule staminali emopoietiche derivate da midollo osseo per abilitare ingegneria cellulare combinata con il Targeting magnetico

While CD133+ hematopoietic stem cells (SCs) have been proven to provide high potential in the field of regenerative medicine, their low retention rates ...

Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Misurazione del potenziale d'azione ottico a singola cellula in cardiomiociti derivati da cellule staminali pluripotenti indotte umane

Conventional intracellular microelectrode techniques to quantify cardiomyocyte electrophysiology are extremely complex, labor intensive, and typically ...