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DOI :
10.3791/57370-v
April 24th, 2018
Chapters
0:04
Title
0:51
Dissection of Epicardial Layer and Isolation of Epicardial Derived Cells
4:10
Culture of EPDCs
5:32
Induction of Epithelial to Mesenchymal Transition in EPDCs
6:31
Results: Isolation of EPDCs from Human Adult Cardiac Tissue and Induction and Validation of EMT in EPDCs
8:17
Conclusion
心外膜在心脏的发育和修复中起着至关重要的作用, 它为心肌壁提供细胞和生长因子。在这里, 我们描述了一种培养人原发性心外膜细胞的方法, 能够对它们的发育和成人特征进行研究和比较。
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