Name: synthesis of multi-walled carbon nanotubes modified with silver nanoparticles and evaluation of their antibacterial activities and cytotoxic properties
Uploaded: 2018-05-10T12:30:00
Description: Watch this Scientific Journal Video about Synthesis of Multi-walled Carbon Nanotubes Modified with Silver Nanoparticles and Evaluation of Their Antibacterial Activities and Cytotoxic Properties at JoV

这项研究得到了 NRF 大学研究补助金 (2016) 和纳米材料技术开发项目的支持, 通过由科学和信息和通信技术部 (2017M3A7B8061942) 资助的韩国国家研究基金会 ()。

1. MWCNT 氧化
<ol><li>测量 30-50 毫克的 MWCNT 成一个50毫升的瓶子。缓慢地添加8毫升的 H2, 所以4: HNO3解决方案 (90% 初始浓度, 3:1 卷/卷) 的吸管与1毫升吸管提示。 注意: 此制剂必须在化学油烟机中进行。<ol>
<li>允许30分钟的放热反应完成。</li>
	</ol>
</li>
	<li>油脂实验溶液在 60-80 °c 和 160 W 为1小时, 直到 MWCNT 安定在瓶的底部。 注意: sonicator 的水位应该低于瓶子的顶端, 这样?...

<ol>
	<li>L&#246;ndahl, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Physical+and+Biological+Properties+of+Bioaerosols.">Physical and Biological Properties of Bioaerosols.</a> Bioaerosol Detection Technologies. , 33-48 (2014).</li><li>Jennings, S., Moran, A., Carroll, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Bioaerosols+and+biofilms.">Bioaerosols and biofilms.</a> Biofilms in medicine, industry and environmental biotechnology. , 160-178 (2003).</li><li>Flemming, H. -. C., Wingender, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+biofilm+matrix.">The biofilm matrix.</a> Nature Reviews Microbiology. 8 (9), 623-633 (2010).</li><li>Lewis, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Riddle+of+biofilm+resistance.">Riddle of biofilm resistance.</a> Antimicrobial agents and chemotherapy. 45 (4), 999-1007 (2001).</li><li>Doronina, N. V., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Methylobacterium+suomiense+sp.+nov.+and+Methylobacterium+lusitanum+sp.+nov.,+aerobic,+pink-pigmented,+facultatively+methylotrophic+bacteria.">Methylobacterium suomiense sp. nov. and Methylobacterium lusitanum sp. nov., aerobic, pink-pigmented, facultatively methylotrophic bacteria.</a> International Journal of Systematic and Evolutionary Microbiology. 52 (3), 773-776 (2002).</li><li>Seo, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Antibacterial+activity+and+cytotoxicity+of+multiwalled+carbon+nanotubes+decorated+with+silver+nanoparticles.">Antibacterial activity and cytotoxicity of multiwalled carbon nanotubes decorated with silver nanoparticles.</a> International Journal of Nanomedicine. 9, 4621-4629 (2014).</li><li>Chen, X., Schluesener, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Nanosilver:+a+nanoproduct+in+medical+application.">Nanosilver: a nanoproduct in medical application.</a> Toxicologyletters. 176 (1), 1-12 (2008).</li><li>Singh, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Nanotechnology+in+medicine+and+antibacterial+effect+of+silver+nanoparticles.">Nanotechnology in medicine and antibacterial effect of silver nanoparticles.</a> Digest Journal of Nanomaterials and Biostructures. 3 (3), 115-122 (2008).</li><li>Morones, J. R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+bactericidal+effect+of+silver+nanoparticles.">The bactericidal effect of silver nanoparticles.</a> Nanotechnology. 16 (10), 2346 (2005).</li><li>Martinez-Castanon, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Synthesis+and+antibacterial+activity+of+silver+nanoparticles+with+different+sizes.">Synthesis and antibacterial activity of silver nanoparticles with different sizes.</a> Journal of Nanoparticle Research. 10 (8), 1343-1348 (2008).</li><li>Lok, C. -. N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Silver+nanoparticles:+partial+oxidation+and+antibacterial+activities.">Silver nanoparticles: partial oxidation and antibacterial activities.</a> JBIC Journal of Biological Inorganic Chemistry. 12 (4), 527-534 (2007).</li></ol>

在这里, 我们报告了一个简单的方法来制备 MWCNTs 与沉积银纳米粒子。这种含银的纳米材料显示了大量的抗菌活性和极少的潜力, 不受控制地吸收银纳米粒子在体内。我们证明, 30 µg/毫升的合成银-MWCNTs 是一个有效的水平的抗菌活性的Methylobacterium. , 对哺乳动物肝细胞的毒性微乎其微。虽然在商业部门扩大之前需要对银 MWCNTs 进行额外的改进和生物安全评估, 但使用乙醇作为减少剂可以帮助生?...

这项研究的最终目的是制造环境友好的抗菌纳米材料, 从而抑制形成生物膜的细菌的生长。这些抗菌纳米材料有可能克服常用化学药品或抗生素化合物的毒性和耐药性问题。生物膜是由多糖、蛋白质、核酸和血脂组成的水合胞外高分子物质 (EPS),1,2。生物膜防止外来物质的侵入, 帮助细菌大力生长3,4。生物膜引起气味和慢性传染性疾病5,6。例如, Methylobacterium通过附着在水始终存在的地方或难以确保连续进行细菌根除 (如空调热交换器、淋浴室和医疗设备) 而生长。这些类型的生物膜引起气味和慢性传染性疾病5,6。
通常, 化学物质或抗生素化合物用于抑制形成生物膜的细菌的生长。抗生素耐药性细菌的出现和对化学品的体内安全性的担忧促使人们需要开发新材料, 以防止生物膜的形成和抑制细菌的生长。
在本研究中, 合成了抗菌纳米材料, 无抗生素耐药性和毒性。银是一种众所周知的抗菌物质, 最近的纳米和纳米技术的发展导致了对金属纳米粒子的抗菌作用的积极研究7,8。最近的研究报告说, 纳米粒子的体积小, 体积比大, 导致抗菌活性增加9,10,11。
本文介绍的纳米材料将银纳米粒子与增强的抗菌性能和碳纳米管结合在一起, 从而增加了单位体积的表面积。制备的银纳米粒子-碳纳米管复合材料具有很大的抗菌性能和对人体和动物细胞的最小毒性。以前研究中的合成过程使用了诸如 NaBH4、甲酰胺、甲基酰胺和肼等有害还原剂。这个过程很复杂, 很危险, 而且耗时。这里报告的合成过程使用乙醇作为一种显著减少危险的还原剂。
测定了银 MWCNTs 的抑菌区和最低杀菌浓度;用活/死和台盼蓝法测定毒性和抗菌性能。最低抑制浓度 (MIC) 和线粒体毒性 (MTT) 检测没有执行, 因为不寻常的颜色的碳纳米管, 这将干扰的化验。最后, 确定了在不影响哺乳动物细胞的情况下, 防止Methylobacterium生长的最小浓度。

<table>
	<tbody>
		<tr>
			<td>0.1 N silver nitrate</td>
			<td>SIGMA-ALDRICH</td>
			<td>1090811000</td>
			<td></td>
		</tr>
		<tr>
			<td>Carbon nanotube, multi-walled</td>
			<td>Tokyo Chemical Industry Co., LTD</td>
			<td>308068-56-6</td>
			<td></td>
		</tr>
		<tr>
			<td>R2A agar</td>
			<td>MBcell</td>
			<td>MB-R1129</td>
			<td></td>
		</tr>
		<tr>
			<td>R2A broth</td>
			<td>MBcell</td>
			<td>MB-R2230</td>
			<td></td>
		</tr>
		<tr>
			<td>Methylobacterium spp.</td>
			<td>KCTC</td>
			<td>12618</td>
			<td>from Korea Collection for Type Cultures Daejeon Korea 12618, Daejon, Korea</td>
		</tr>
		<tr>
			<td>LIVE/DEAD Cell imaging Kit</td>
			<td>ThermoFisher SCIENTIFIC</td>
			<td>R37601</td>
			<td></td>
		</tr>
		<tr>
			<td>AML12</td>
			<td></td>
			<td></td>
			<td>from Chungnam University, Dajeon, Korea</td>
		</tr>
		<tr>
			<td>human PBMC</td>
			<td>ATCC</td>
			<td>PCS-800-011</td>
			<td></td>
		</tr>
		<tr>
			<td>TEM</td>
			<td>JEOL</td>
			<td>JEM-2100F</td>
			<td></td>
		</tr>
		<tr>
			<td>XRD</td>
			<td>Rigaku</td>
			<td>D/MAX 2500</td>
			<td>Cu K photon source (40kV, 100mA)</td>
		</tr>
		<tr>
			<td>JuLI Br</td>
			<td>NanoEnTek</td>
			<td>JULI-BRSC&#160;</td>
			<td></td>
		</tr>
	</tbody>
</table>

synthesis of multi-walled carbon nanotubes modified with silver nanoparticles and evaluation of their antibacterial activities and cytotoxic properties

本研究采用水硫酸溶液处理多壁碳纳米管 (MWCNTs), 形成氧基功能基团。银 MWCNTs 是由还原沉积银从 AgNO3的水溶液中氧化 MWCNTs。鉴于碳纳米管的独特颜色, 不可能将其应用于最小抑制浓度或线粒体毒性检测, 以评估毒性和抗菌性能, 因为它们会干扰化验。测定了银 MWCNTs 的抑菌区和最低杀菌浓度, 并利用台盼蓝法测定了其毒性和抗菌性能, 不影响碳纳米管的颜色。

透射电镜 (TEM) 图像确认银 MWCNTs 的形成 (图 1A 和 1B)。表面电荷的变化证实了它们的成功合成。计算了 MWCNTs 上沉积的 Ag 粒子的大小 (图 1C)。平均微粒大小是大约3.83 毫微米。该合成银 MWCNTs 的 XRD 模式显示在图 1D中。峰值在 20-30°对应于 MWCNT;其余的峰值对应于 Ag。抗菌活动数据显示在<strong class...

Watch this Scientific Journal Video about Synthesis of Multi-walled Carbon Nanotubes Modified with Silver Nanoparticles and Evaluation of Their Antibacterial Activities and Cytotoxic Properties at JoV

Synthesis of Multi-walled Carbon Nanotubes Modified with Silver Nanoparticles and Evaluation of Their Antibacterial Activities and Cytotoxic Properties

本研究通过多壁纳米碳纳米管的酸性氧化和银纳米粒子随后的还原沉积合成了抗菌纳米材料。用制备的纳米材料进行抗菌活性和细胞毒性试验。

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