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DOI :
10.3791/57431-v
•
9:20 min
April 27th, 2018
Chapters
0:04
Title
1:05
Homogenization
4:40
Microglia Immunostaining
6:41
Results: Isolation of Neurons, Macrophages, and Microglia, and their RNA from Larval Zebrafish Brains
8:25
Conclusion
我们提出了一种在生理和病理条件下, 从斑马鱼脑中分离出神经元、巨噬细胞和小胶质的协议。分离后, 从这些细胞中提取 RNA, 分析其基因表达谱。该协议允许收集高质量的 RNA 来执行下游分析, 如 qPCR 和转录组学。
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