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DOI :
10.3791/57460-v
October 9th, 2018
Chapters
0:04
Title
0:33
Preparation for Tumor Transplantation
1:40
Surgical Operation for Orthotopic Implantation
2:58
Tumor Tissue Dissociation and Single Cell Preparation
5:01
Flow Data Acquisition
6:46
Results: Comparison of Orthotopic and Subcutaneous Implantation of Pancreatic Ductal Adenocarcinoma
8:04
Conclusion
免疫分型小鼠原位 PDAC homografts 的实验程序旨在分析肿瘤免疫微环境。肿瘤通过手术原位植入。200–600毫米3的肿瘤被收获和分离, 以制备单细胞悬浮液, 然后使用不同的荧光标记抗体进行多免疫标记物的分析。
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