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流式细胞术对小鼠原发性胰腺导管腺癌原位同种异体 (自体) 免疫分型的研究

DOI :

10.3791/57460-v

October 9th, 2018

October 9th, 2018

11,835 Views

1Crown Bioscience Inc., 2State Key Laboratory of Natural and Biomimetic Drugs, Peking University

免疫分型小鼠原位 PDAC homografts 的实验程序旨在分析肿瘤免疫微环境。肿瘤通过手术原位植入。200–600毫米3的肿瘤被收获和分离, 以制备单细胞悬浮液, 然后使用不同的荧光标记抗体进行多免疫标记物的分析。

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