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DOI :
10.3791/57460-v
October 9th, 2018
Chapters
0:04
Title
0:33
Preparation for Tumor Transplantation
1:40
Surgical Operation for Orthotopic Implantation
2:58
Tumor Tissue Dissociation and Single Cell Preparation
5:01
Flow Data Acquisition
6:46
Results: Comparison of Orthotopic and Subcutaneous Implantation of Pancreatic Ductal Adenocarcinoma
8:04
Conclusion
実験マウスの同所性同種 PDAC 移植の診療には、腫瘍免疫微小環境のプロファイリングを目指しています。腫瘍は、手術を介して注入したがんです。200-600 mm3の大きさの腫瘍は、収穫され、異なる蛍光標識抗体を用いて免疫マルチ マーカー FACS 解析に続いて単一細胞懸濁液を準備する解離。
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