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DOI :
10.3791/57462-v
•
13:05 min
May 7th, 2018
Chapters
0:04
Title
1:07
Isolation of Primary Mammary Cell Populations
4:07
Purification of Mammary Epithelial Cells by FACS and Seeding of Primary Mammary LD Cells
5:05
Induction of YAP-induced Mammary Stem Cells and Sub-culturing of yMaSCs
7:33
Generation of yDucts
9:57
Results: Reprogramming Primary Mammary LD Epithelial Cells into Mammary Stem Cells
11:25
Conclusion
在再生医学、疾病建模和了解 SC 特性方面, 可获得的躯体 SCs 是至关重要的。在这里, 我们提出的实验策略, 以重新编程,在体外,分化成人细胞成相应的可膨胀的组织特定的茎/祖细胞由单转录联合激活剂狂吠的瞬态表达。
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