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DOI :
10.3791/57462-v
•
13:05 min
May 7th, 2018
Chapters
0:04
Title
1:07
Isolation of Primary Mammary Cell Populations
4:07
Purification of Mammary Epithelial Cells by FACS and Seeding of Primary Mammary LD Cells
5:05
Induction of YAP-induced Mammary Stem Cells and Sub-culturing of yMaSCs
7:33
Generation of yDucts
9:57
Results: Reprogramming Primary Mammary LD Epithelial Cells into Mammary Stem Cells
11:25
Conclusion
体 SCs の可用性は再生医療の重要な疾患モデル作製と SC プロパティへの洞察力を得るために。再プログラムする実験的戦略の紹介、体外が単一の転写コアクチベーター ヤップの一過性の発現によって彼らの対応する拡張幹/前駆細胞の細胞に成体細胞を区別します。
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