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DOI :
10.3791/57470-v
June 23rd, 2018
Chapters
0:05
Title
1:10
S. cerevisiae Cultures and Protein Expression
3:16
Cell Staining
4:29
Flow Cytometry Analysis
9:05
Results: Protein Inclusions Play a Protective Role Against Oxidative Stress
10:38
Conclusion
蛋白质聚集诱发细胞氧化应激。本协议描述了一种通过流式细胞仪监测 amyloidogenic 蛋白的细胞内状态和与之相关的氧化应激的方法。该方法用于研究淀粉样β肽的可溶性和聚集易变性的行为。
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