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DOI :
10.3791/57471-v
May 5th, 2018
Chapters
0:04
Title
0:39
3' Barcoded Adapter Ligations with 18 Individual RNA Samples
2:00
Ligated Small RNA Purification
5:26
5' Ligated and 3' Barcoded Purified Small RNAs Reverse Transcription
8:12
Pilot PCR and Large-scale PCR Amplification
10:46
Results: Protocol Robust Whether Fresh, Frozen, or Aged
12:01
Conclusion
ホルマリン固定パラフィン包埋標本を表す人間の病気の分子バイオ マーカーの貴重な情報源。実験に基づく cDNA ライブラリの準備プロトコル、最初に新鮮な冷凍 RNA をデザインおよび最適化組織からアーカイブされたマイクロ Rna の解析に格納された 35 歳までをご紹介します。
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