Hadassah - Hebrew University Medical Center

2 ARTICLES PUBLISHED IN JoVE

Genetics

Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens

Olivier Loudig^1,2,3, Christina Liu^1,2, Thomas Rohan³, Iddo Z. Ben-Dov⁴

¹Department of Research, Hackensack University Medical Center, ²Department of Medical Sciences, Seton Hall University, ³Department of Epidemiology and Population Health, Albert Einstein College of Medicine, ⁴Department of Nephrology and Hypertension, Hadassah - Hebrew University Medical Center

Formalin-fixed paraffin-embedded specimens represent a valuable source of molecular biomarkers of human diseases. Here we present a laboratory-based cDNA library preparation protocol, initially designed with fresh frozen RNA, and optimized for the analysis of archived microRNAs from tissues stored up to 35 years.

Biology

A BW Reporter System for Studying Receptor-Ligand Interactions

Shlomo Elias^1,2, Shira Kahlon², Alexandra Duev-Cohen², Ofer Mandelboim²

¹Department of Hematology, Hadassah - Hebrew University Medical Center, ²The Lautenberg Center for General and Tumor Immunology, Department of Immunology and Cancer Research, Institute for Medical Research Israel Canada (IMRIC), Hadassah - Hebrew University Medical Center

This protocol describes how to establish a reporter system that can be used to identify and quantify receptor-ligand interactions.