Name: co-transplantation of human ovarian tissue with engineered endothelial cells: a cell-based strategy combining accelerated perfusion with direct paracrine delivery
Uploaded: 2018-05-16T14:00:00
Description: Watch this Scientific Journal Video about Co-transplantation of Human Ovarian Tissue with Engineered Endothelial Cells: A Cell-based Strategy Combining Accelerated Perfusion with Direct Paracrine Deli

Omar Alexander adam resimler için. Translational bilim merkezi ve bir ASRM grant araştırma ve L.M. Cornell klinik bir Pilot Ödülü tarafından desteklenmiştir. Yazar James laboratuvar üyeleri şekilde alt alta yazılmalıdır eleştirel okuma için teşekkür etmek istiyorum.

Tüm yordamları hayvan konular içeren kurumsal hayvan bakım ve kullanım Komitesi (IACUC) Weill Cornell Medical College tarafından onaylanmıştır. Yumurtalık dokusu kullanarak tüm xenotransplantation deneyleri ilgili kurallara ve düzenlemelere uygun şekilde yapıldı. İnsan yumurtalık dokusu kemoterapi veya radyoterapi kanser tedavisi veya önceden kemik iliği nakli için planlanan hastaların toplanmıştır. Kurumsal inceleme Kurulu (IRB) Komitesi, Weill Cornell Tıp Koleji doku potansiyel Otolog kullanıl...

<ol>
	<li>Siegel, R. L., Miller, K. D., Jemal, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cancer+Statistics,+2017.">Cancer Statistics, 2017.</a> CA Cancer J Clin. 67 (1), 7-30 (2017).</li><li>Magelssen, H., Melve, K. K., Skjaerven, R., Fossa, S. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Parenthood+probability+and+pregnancy+outcome+in+patients+with+a+cancer+diagnosis+during+adolescence+and+young+adulthood.">Parenthood probability and pregnancy outcome in patients with a cancer diagnosis during adolescence and young adulthood.</a> Hum Reprod. 23 (1), 178-186 (2008).</li><li>Donnez, J., Dolmans, M. M., Diaz, C., Pellicer, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ovarian+cortex+transplantation:+time+to+move+on+from+experimental+studies+to+open+clinical+application.">Ovarian cortex transplantation: time to move on from experimental studies to open clinical application.</a> Fertil Steril. 104 (5), 1097-1098 (2015).</li><li>Stoop, D., Cobo, A., Silber, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fertility+preservation+for+age-related+fertility+decline.">Fertility preservation for age-related fertility decline.</a> Lancet. 384 (9950), 1311-1319 (2014).</li><li>Aubard, Y., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Orthotopic+and+heterotopic+autografts+of+frozen-thawed+ovarian+cortex+in+sheep.">Orthotopic and heterotopic autografts of frozen-thawed ovarian cortex in sheep.</a> Hum Reprod. 14 (8), 2149-2154 (1999).</li><li>Newton, H., Aubard, Y., Rutherford, A., Sharma, V., Gosden, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Low+temperature+storage+and+grafting+of+human+ovarian+tissue.">Low temperature storage and grafting of human ovarian tissue.</a> Hum Reprod. 11 (7), 1487-1491 (1996).</li><li>Van Eyck, A. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Electron+paramagnetic+resonance+as+a+tool+to+evaluate+human+ovarian+tissue+reoxygenation+after+xenografting.">Electron paramagnetic resonance as a tool to evaluate human ovarian tissue reoxygenation after xenografting.</a> Fertil Steril. 92 (1), 374-381 (2009).</li><li>Nugent, D., Newton, H., Gallivan, L., Gosden, R. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Protective+effect+of+vitamin+E+on+ischaemia-reperfusion+injury+in+ovarian+grafts.">Protective effect of vitamin E on ischaemia-reperfusion injury in ovarian grafts.</a> J Reprod Fertil. 114 (2), 341-346 (1998).</li><li>Kim, S. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Quantitative+assessment+of+ischemic+tissue+damage+in+ovarian+cortical+tissue+with+or+without+antioxidant+(ascorbic+acid)+treatment.">Quantitative assessment of ischemic tissue damage in ovarian cortical tissue with or without antioxidant (ascorbic acid) treatment.</a> Fertil Steril. 82 (3), 679-685 (2004).</li><li>Abir, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Improving+posttransplantation+survival+of+human+ovarian+tissue+by+treating+the+host+and+graft.">Improving posttransplantation survival of human ovarian tissue by treating the host and graft.</a> Fertil Steril. 95 (4), 1205-1210 (2011).</li><li>Friedman, O., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Possible+improvements+in+human+ovarian+grafting+by+various+host+and+graft+treatments.">Possible improvements in human ovarian grafting by various host and graft treatments.</a> Hum Reprod. 27 (2), 474-482 (2012).</li><li>Shikanov, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fibrin+encapsulation+and+vascular+endothelial+growth+factor+delivery+promotes+ovarian+graft+survival+in+mice.">Fibrin encapsulation and vascular endothelial growth factor delivery promotes ovarian graft survival in mice.</a> Tissue Eng Part A. 17 (23-24), 3095-3104 (2011).</li><li>Soleimani, R., Heytens, E., Oktay, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Enhancement+of+neoangiogenesis+and+follicle+survival+by+sphingosine-1-phosphate+in+human+ovarian+tissue+xenotransplants.">Enhancement of neoangiogenesis and follicle survival by sphingosine-1-phosphate in human ovarian tissue xenotransplants.</a> PLoS One. 6 (4), e19475 (2011).</li><li>Israely, T., Dafni, H., Nevo, N., Tsafriri, A., Neeman, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Angiogenesis+in+ectopic+ovarian+xenotransplantation:+multiparameter+characterization+of+the+neovasculature+by+dynamic+contrast-enhanced+MRI.">Angiogenesis in ectopic ovarian xenotransplantation: multiparameter characterization of the neovasculature by dynamic contrast-enhanced MRI.</a> Magn Reson Med. 52 (4), 741-750 (2004).</li><li>Buratini, J., Price, C. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Follicular+somatic+cell+factors+and+follicle+development.">Follicular somatic cell factors and follicle development.</a> Reprod Fertil Dev. 23 (1), 32-39 (2011).</li><li>Dunlop, C. E., Anderson, R. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+regulation+and+assessment+of+follicular+growth.">The regulation and assessment of follicular growth.</a> Scand J Clin Lab Invest Suppl. 244, 13-17 (2014).</li><li>Durlinger, A. L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Control+of+primordial+follicle+recruitment+by+anti-M&#252;llerian+hormone+in+the+mouse+ovary.">Control of primordial follicle recruitment by anti-M&#252;llerian hormone in the mouse ovary.</a> Endocrinology. 140 (12), 5789-5796 (1999).</li><li>Schmidt, K. L., Ernst, E., Byskov, A. G., Nyboe Andersen, A., Yding Andersen, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Survival+of+primordial+follicles+following+prolonged+transportation+of+ovarian+tissue+prior+to+cryopreservation.">Survival of primordial follicles following prolonged transportation of ovarian tissue prior to cryopreservation.</a> Hum Reprod. 18 (12), 2654-2659 (2003).</li><li>Jensen, A. K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Outcomes+of+transplantations+of+cryopreserved+ovarian+tissue+to+41+women+in+Denmark.">Outcomes of transplantations of cryopreserved ovarian tissue to 41 women in Denmark.</a> Hum Reprod. 30 (12), 2838-2845 (2015).</li><li>Oktay, K., Newton, H., Aubard, Y., Salha, O., Gosden, R. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cryopreservation+of+immature+human+oocytes+and+ovarian+tissue:+an+emerging+technology?.">Cryopreservation of immature human oocytes and ovarian tissue: an emerging technology?.</a> Fertil Steril. 69 (1), 1-7 (1998).</li><li>Shultz, L. D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Human+lymphoid+and+myeloid+cell+development+in+NOD/LtSz-scid+IL2R+gamma+null+mice+engrafted+with+mobilized+human+hemopoietic+stem+cells.">Human lymphoid and myeloid cell development in NOD/LtSz-scid IL2R gamma null mice engrafted with mobilized human hemopoietic stem cells.</a> J Immunol. 174 (10), 6477-6489 (2005).</li><li>Ramalingam, R., Rafii, S., Worgall, S., Brough, D. E., Crystal, R. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=E1(-)E4(+)+adenoviral+gene+transfer+vectors+function+as+a+&amp;#34;pro-life&amp;#34;+signal+to+promote+survival+of+primary+human+endothelial+cells.">E1(-)E4(+) adenoviral gene transfer vectors function as a &amp;#34;pro-life&amp;#34; signal to promote survival of primary human endothelial cells.</a> Blood. 93 (9), 2936-2944 (1999).</li><li>Seandel, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Generation+of+a+functional+and+durable+vascular+niche+by+the+adenoviral+E4ORF1+gene.">Generation of a functional and durable vascular niche by the adenoviral E4ORF1 gene.</a> Proc Natl Acad Sci U S A. 105 (49), 19288-19293 (2008).</li><li>Meirow, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cortical+fibrosis+and+blood-vessels+damage+in+human+ovaries+exposed+to+chemotherapy.+Potential+mechanisms+of+ovarian+injury.">Cortical fibrosis and blood-vessels damage in human ovaries exposed to chemotherapy. Potential mechanisms of ovarian injury.</a> Hum Reprod. 22 (6), 1626-1633 (2007).</li><li>Assidi, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Identification+of+potential+markers+of+oocyte+competence+expressed+in+bovine+cumulus+cells+matured+with+follicle-stimulating+hormone+and/or+phorbol+myristate+acetate+in+vitro.">Identification of potential markers of oocyte competence expressed in bovine cumulus cells matured with follicle-stimulating hormone and/or phorbol myristate acetate in vitro.</a> Biol Reprod. 79 (2), 209-222 (2008).</li><li>Thakur, S. C., Datta, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Higher+expression+of+hyaluronan+binding+protein+1+(HABP1/p32/gC1qR/SF2)+during+follicular+development+and+cumulus+oocyte+complex+maturation+in+rat.">Higher expression of hyaluronan binding protein 1 (HABP1/p32/gC1qR/SF2) during follicular development and cumulus oocyte complex maturation in rat.</a> Mol Reprod Dev. 75 (3), 429-438 (2008).</li><li>Dolmans, M. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Short-term+transplantation+of+isolated+human+ovarian+follicles+and+cortical+tissue+into+nude+mice.">Short-term transplantation of isolated human ovarian follicles and cortical tissue into nude mice.</a> Reproduction. 134 (2), 253-262 (2007).</li><li>Amorim, C. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Impact+of+freezing+and+thawing+of+human+ovarian+tissue+on+follicular+growth+after+long-term+xenotransplantation.">Impact of freezing and thawing of human ovarian tissue on follicular growth after long-term xenotransplantation.</a> J Assist Reprod Genet. 28 (12), 1157-1165 (2011).</li><li>Kawamura, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hippo+signaling+disruption+and+Akt+stimulation+of+ovarian+follicles+for+infertility+treatment.">Hippo signaling disruption and Akt stimulation of ovarian follicles for infertility treatment.</a> Proc Natl Acad Sci U S A. 110 (43), 17474-17479 (2013).</li><li>Suzuki, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Successful+fertility+preservation+following+ovarian+tissue+vitrification+in+patients+with+primary+ovarian+insufficiency.">Successful fertility preservation following ovarian tissue vitrification in patients with primary ovarian insufficiency.</a> Hum Reprod. 30 (3), 608-615 (2015).</li><li>Campbell, B. K., Clinton, M., Webb, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+role+of+anti-M&#252;llerian+hormone+(AMH)+during+follicle+development+in+a+monovulatory+species+(sheep).">The role of anti-M&#252;llerian hormone (AMH) during follicle development in a monovulatory species (sheep).</a> Endocrinology. 153 (9), 4533-4543 (2012).</li><li>Donnez, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Restoration+of+ovarian+activity+and+pregnancy+after+transplantation+of+cryopreserved+ovarian+tissue:+a+review+of+60+cases+of+reimplantation.">Restoration of ovarian activity and pregnancy after transplantation of cryopreserved ovarian tissue: a review of 60 cases of reimplantation.</a> Fertil Steril. 99 (6), 1503-1513 (2013).</li><li>Ferreira, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+effects+of+sample+size+on+the+outcome+of+ovarian+tissue+cryopreservation.">The effects of sample size on the outcome of ovarian tissue cryopreservation.</a> Reprod Domest Anim. 45 (1), 99-102 (2010).</li><li>Gavish, Z., Peer, G., Roness, H., Cohen, Y., Meirow, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Follicle+activation+and+'burn-out'+contribute+to+post-transplantation+follicle+loss+in+ovarian+tissue+grafts:+the+effect+of+graft+thickness.">Follicle activation and 'burn-out' contribute to post-transplantation follicle loss in ovarian tissue grafts: the effect of graft thickness.</a> Hum Reprod. 30 (4), 1003 (2015).</li><li>Donnez, J., Dolmans, M. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fertility+Preservation+in+Women.">Fertility Preservation in Women.</a> N Engl J Med. 377 (17), 1657-1665 (2017).</li><li>Salama, M., Woodruff, T. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=New+advances+in+ovarian+autotransplantation+to+restore+fertility+in+cancer+patients.">New advances in ovarian autotransplantation to restore fertility in cancer patients.</a> Cancer Metastasis Rev. 34 (4), 807-822 (2015).</li><li>Donnez, J., Dolmans, M. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ovarian+cortex+transplantation:+60+reported+live+births+brings+the+success+and+worldwide+expansion+of+the+technique+towards+routine+clinical+practice.">Ovarian cortex transplantation: 60 reported live births brings the success and worldwide expansion of the technique towards routine clinical practice.</a> J Assist Reprod Genet. 32 (8), 1167-1170 (2015).</li><li>Meirow, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Transplantations+of+frozen-thawed+ovarian+tissue+demonstrate+high+reproductive+performance+and+the+need+to+revise+restrictive+criteria.">Transplantations of frozen-thawed ovarian tissue demonstrate high reproductive performance and the need to revise restrictive criteria.</a> Fertil Steril. 106 (2), 467-474 (2016).</li><li>Kalich-Philosoph, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cyclophosphamide+triggers+follicle+activation+and+"burnout";+AS101+prevents+follicle+loss+and+preserves+fertility.">Cyclophosphamide triggers follicle activation and "burnout"; AS101 prevents follicle loss and preserves fertility.</a> Sci Transl Med. 5 (185), 185ra162 (2013).</li><li>Kano, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=AMH/MIS+as+a+contraceptive+that+protects+the+ovarian+reserve+during+chemotherapy.">AMH/MIS as a contraceptive that protects the ovarian reserve during chemotherapy.</a> Proc Natl Acad Sci U S A. 114 (9), E1688-E1697 (2017).</li></ol>

Burada biz exECs Co o nakli göstermek yumurtalık dokusu canlılık ve xenograft farelerde aşağıdaki işlevi önemli bir yarar sağlar. Yumurtalık dokusu auto-nakli doğurganlık korunmasına ilişkin klinik uygulama için standartlar kümesi ve optimum parametreleri (boyut, nakli sitesi, süresi greft, vb) olmamıştır 32 , 33 , 34 foliküler havuzun gelişmiş kurtarma için tanımsız kalır. Auto-ekimi yapıl?...

Kanser gelişmiş dünyada önde gelen ölüm nedenleri arasında kalır, henüz onlarca yıllık araştırma kanser ve bazı durumlarda neredeyse iki katına hayatta kalma oranları1, birçok türü için önemli bir ilerleme vermiştir. Ne yazık ki, kemoterapötik ajanlar gonadotoxic, yumurtalıklarda ilkel köklerinin rezerv tüketen ve doğurganlık2azaltılması çoğu kez. Bu büyüyen nüfusun doğurganlık koruma oosit ve/veya embriyo dondurma da dahil olmak üzere çeşitli yöntemler yararlanabilir, ancak, kanser tedavisi ve önceden pubertal hastaların istem başlatma gerektiren hastalar için bu seçenekleri geçersiz. Alternatif olarak, bazı hastalarda yumurtalık dokusu onların tedavi rejimi girişmeden önce ve kurtarma ve remisyon, doğurganlık3geri yüklemek için otomatik dikim doku üzerine cryopreserve seçtiniz. Henüz, bugüne kadar greft hayatta kalma ve foliküler çıkış auto-nakli takip nispeten düşük4, ana toprak dolay iden doku iskemi ve hipoksi5,6,7kalır. Yumurtalık kortikal Greftler canlılık geliştirmek için çok sayıda çabalarına rağmen anti-oksidanlar8,9, pro-anjiogenik sitokinler10,11,12,1 kullanarak 5-7 gün pencere sonrası organ nakli 3veya mekanik işlemler14, greft iskemi canlılığı ve greft7yaşama zayıflatmaktadır. Bu sorunu çözmek için ev sahibi ve greft damarlarının anastomoz kolaylaştırmak ve böylece reperfüzyon Yumurtalık dokusunun hızlandırmak için bir hücre tabanlı strateji geliştirdi.
Sonrası organ nakli penceresinde aşılı yumurtalık dokusu için iskemik hakaret yanı sıra arası foliküler sinyal kesintileri havuzu15,16tükenmesi için katkıda bulunabilir. Eksojen endotel hücreleri (ExECs) istikrarlı ve düzgün damarları greft çevre için katkıda çünkü, onlar nakledilen doku tanımlanmış bir moleküler girişine iletmek için eşsiz bir fırsat sunuyoruz. İlke kanıtı olarak Anti-Mullerian hormon (AMH), foliküler büyüme17kısıtlamak için gösterilen dönüştürme büyüme faktörü beta (TGFβ) üst aile üyesi hızlı süper fizyolojik düzeylere ExECs mühendislik. Karşılaştırma kontrol ve AMH ifade hücreleri ile birlikte nakledilen Greftler foliküler dağıtım biyolojik aktivite ve mühendislik exECs potens doğrular.
Özetle, greft canlılığı ve baskılayarak foliküler Havuzu, erken seferberlik geliştirerek bu yaklaşım doğurganlık koruma uygulanan hastalarda yumurtalık dokusu otomatik nakledilen verimliliğini artırabilirsiniz. Ayrıca, foliküler gelişim karıştığı moleküler düzenleyiciler, deneysel sorgulama ExEC tabanlı platformu sağlar.

<table>
	<tbody>
		<tr>
			<td>Leibovitz&#8217;s L-15 medium</td>
			<td>Gibco</td>
			<td>11415064</td>
			<td></td>
		</tr>
		<tr>
			<td>Antibiotic-Antimycotic</td>
			<td>Gibco</td>
			<td>15240062</td>
			<td>Anti-Anti X100</td>
		</tr>
		<tr>
			<td>Sucrose</td>
			<td>Sigma</td>
			<td>S 1888</td>
			<td></td>
		</tr>
		<tr>
			<td>Fibrinogen</td>
			<td>Sigma</td>
			<td>F 8630</td>
			<td>from bovine plasma</td>
		</tr>
		<tr>
			<td>Thrombin</td>
			<td>Sigma</td>
			<td>T 1063</td>
			<td>from human plasma</td>
		</tr>
		<tr>
			<td>DMSO</td>
			<td>Sigma</td>
			<td>D 2650</td>
			<td></td>
		</tr>
		<tr>
			<td>DMEM</td>
			<td>Gibco</td>
			<td>12491015</td>
			<td></td>
		</tr>
		<tr>
			<td>Enzyme Cell Detachment Medium</td>
			<td>Invitrogen</td>
			<td>00-4555-56</td>
			<td>Accutase</td>
		</tr>
		<tr>
			<td>Plastic paraffin film</td>
			<td>Bemis NA</td>
			<td></td>
			<td>Parafilm M</td>
		</tr>
		<tr>
			<td>Surgical paper tape 2.5 cm</td>
			<td>3M</td>
			<td>1530-1</td>
			<td>Micropore</td>
		</tr>
		<tr>
			<td>Surgical Paper tape 1.25 cm</td>
			<td>3M</td>
			<td>1530-0</td>
			<td>Micropore</td>
		</tr>
		<tr>
			<td>Perforated plastic Surgical tape 1.25 cm</td>
			<td>3M</td>
			<td>1527-0</td>
			<td>Transpore</td>
		</tr>
		<tr>
			<td>Monofilament Absorbable Suture</td>
			<td>Covidien</td>
			<td>UM-203</td>
			<td>Biosyn</td>
		</tr>
		<tr>
			<td>Braided Absorbable Suture</td>
			<td>Covidien</td>
			<td>GL-889</td>
			<td>Polysorb</td>
		</tr>
		<tr>
			<td>Povidone-iodine Solution USP 10%</td>
			<td>Purdue Products</td>
			<td>67618-153-01</td>
			<td>Betadine Solution Swab Stick</td>
		</tr>
		<tr>
			<td>Cryoviales</td>
			<td>Nunc</td>
			<td>377267</td>
			<td>CryoTube</td>
		</tr>
		<tr>
			<td>sterile ocular lubricant</td>
			<td>Dechra</td>
			<td>17033-211-38</td>
			<td>Puralube</td>
		</tr>
		<tr>
			<td>1.7 ml micro-centrifuge tube</td>
			<td>Denville</td>
			<td>C-2172</td>
			<td>Eppendorf</td>
		</tr>
		<tr>
			<td>Anasthesia system</td>
			<td>VetEquip</td>
			<td>V-1 table top system with scavenging</td>
			<td></td>
		</tr>
		<tr>
			<td>Endothelial cells</td>
			<td>Angiocrine Biosciences, Inc., San Diego, CA, USA</td>
			<td></td>
			<td>Isolated, transfected with E4-ORF- 1 and labeled endothelial cells</td>
		</tr>
		<tr>
			<td>Trichrome stain</td>
			<td>Sigma</td>
			<td>HT15-1kt</td>
			<td>Trichrome Stain (Masson) Kit</td>
		</tr>
		<tr>
			<td>Isolectin</td>
			<td>Invitrogen</td>
			<td>I32450</td>
			<td>isolectin GS-IB4 From Griffonia simplicifolia, Alexa Fluor&#8482; 647 Conjugate</td>
		</tr>
	</tbody>
</table>

co-transplantation of human ovarian tissue with engineered endothelial cells: a cell-based strategy combining accelerated perfusion with direct paracrine delivery

İnfertilite, kemoterapi ve/veya radyoterapi ve bazı hastalar sık yan etkisi, yumurta veya embriyo dondurma bir seçenek değil. Alternatif olarak, bu hastalar giderek artan sayıda otogrefti için yumurtalık dokusu cryopreserve için tercih ediyor kurtarma ve remisyon aşağıdaki. Auto-nakli cryopreserved Yumurtalık dokusunun geçiren hastalar arasında istenmeyen sonuçlara gelişmelere rağmen büyük bir engel etkili revaskülarizasyon aşılı doku kalır. İskemi azaltmak ve böylece auto-nakli uygulanan hastalarda sonuçlar geliştirmek için perfüzyon Yumurtalık dokusunun hızlanan bir vasküler hücre tabanlı strateji geliştirdi. Eksojen endotel hücreleri (ExECs) Co nakli için bir yöntem bir fare xenograft model cryopreserved yumurtalık dokusu ile açıklamak. Biz Anti-Mullerian hormon (AMH), yapısal ifade etmek için tasarlanmış böylece sürekli parakrin yumurtalık Greftler için giriş sinyal etkinleştirme ExECs istihdam için bu yaklaşım uzatmak. Foliküler hacmi ve geliştirilmiş antral folikül gelişim ExECs ile ortak ekimi artmış ve saklama deneniyor ilkel köklerinin AMH ifade ExECs terfi. Bu kombine strateji bağlamında foliküler aktivasyonu doğurganlık koruma ve/veya infertilite büyük oransal ve iskemi Azaltıcı için yararlı bir araç olabilir.

ExECs co nakli hastaların doku bir yarar sağlayıp sağlamadığını belirlemek için çözülmüş yumurtalık kortikal şeritler eşit büyüklükte parçalara bölünmüş ve bilateral IMMUNO şaibeli, NOD scid gama (NSG), fare engrafted. Fibrin pıhtısı yalnız (ECs) ve diğer içeren ExECs (Şekil 1a) gömülü bir tarafı ile her fare kendi kontrolü görev yaptı. ExECs insan göbek bağları ve Adenoviral gen parça E4-ORF1, sonraki tedaviyle yuk...

Watch this Scientific Journal Video about Co-transplantation of Human Ovarian Tissue with Engineered Endothelial Cells: A Cell-based Strategy Combining Accelerated Perfusion with Direct Paracrine Deli

Co-transplantation of Human Ovarian Tissue with Engineered Endothelial Cells: A Cell-based Strategy Combining Accelerated Perfusion with Direct Paracrine Delivery

Bazı hastalar için dondurma Yumurtalık dokusunun doğurganlık korunması için tek seçenektir. Ne yazık ki, gecikmiş revaskülarizasyon foliküler canlılığı zayıflatmaktadır. Burada, co için doğrudan parakrin teslim ile hızlandırılmış kullanımı olarak birleştiren bir hücre tabanlı strateji perfüzyon biyoaktif moleküllerinin endotel hücreleri ile insan yumurtalık dokusu nakli için bir protokol mevcut.

İnsan yumurtalık dokusu mühendislik endotel hücreleri ile ortak nakli: bir hücre tabanlı strateji birleştirerek perfüzyon doğrudan parakrin teslim ile hızlandırılmış

Infertility is a frequent side effect of chemotherapy and/or radiotherapy and for some patients, cryopreservation of oocytes or embryos is not an option. ...

The overall goal of this protocol is to xenograft cryopreserved human ovarian tissue in combination with engineered endothelial cells that accelerate re-perfusion and provide paracrine stimulus that modulates follicular activation within the graft. This method provides a cell-based means of improving post-transplant viability of cryopreserved ovarian tissue and more broadly, establishes a robust model system for interrogating molecular regulation of human folliculogenesis. The main advantage of this technique is that it uses a single vehicle that of endothelial cells to both mitigate ischemia and provide a sustained source of secreted factors to engraft the tissue.So this method provide global benefit for tissue viability. It can also provide insight into molecular regulators that have been implicated in follicular development. To make a fibrin clot, work quickly and add 360 microliters of HEPES buffer to 200 microliters of the fibrinogen stock solution.Gently pipette to mix and keep the tube on ice. In the second microfuge tube, prepare thrombin working solution by adding 148.7 microliters of DMEM to 50 microliters of thrombin stock solution. Gently pipette to mix.Then add 1.3 microliters of one mole per liter of calcium chloride. Gently pipette to mix and keep the sample on ice. Using the enzyme cell detachment medium Accutase, detach previously prepared engineered endothelial cells from the plate and transfer them to a third microfuge tube.Then, spin down the cells. Next, using a hemocytometer and a covered glass, count the cells. To calculate the number of cells needed for the engraftment, convert a 20, 000 times area of ovarian tissue into millimeter squared by using a ruler to measure the dimensions.The multiply the area by 20, 000 to determine the number of cells needed for the engraftment. Spin down the required cells. And use DMEM to re-suspend the pellet for a total volume of 16.8 microliters.Keep the cell suspension on ice. Next, place a piece of ovarian tissue on a sterile gauze sponge for a few seconds to dry it. Then transfer the tissue onto a previously prepared plastic paraffin film in a 50 millimeter petri dish.Add 39.2 microliters of the fibrinogen solution into the prepared cell suspension. Mix by gentle pipetting, and keep the suspension on ice. Then working quickly, add 14 microliters of the thrombin solution to the tube and gently pipette once to mix.Pipette the mixture on top of the ovarian tissue in the shape of an elongated droplet. Incubate the petri dish with the clot at 37 degrees Celsius. Work quickly once you have added the thrombin solution to the fibrinogen cell suspension.Synchronizing the future preparation in surgery is a challenge and it may be prudent to perform a practice run prior to expending precious human tissue. After anesthetizing the 10 to 14 week old female NSG mouse, and preparing it for surgery according to the text protocol, heat the animal and monitor the body temperature throughout the procedure. With a povidone iodine swab stick, clean the trimmed area and then use sterile alcohol to wipe it off.Next, place a drop of sterile ocular lubricant vet ointment over each of the mouse's eyes to protect them from dehydration and damage to the cornea. After performing a bilateral oophorectomy according to the text protocol, inject buprenorphine subcutaneous. Then make a horizontal incision in the fascia above the gluteus maximus at a length that fits the clot dimensions.Create a pocket within this virtual space by opening the scissors underneath the fascia. Pick up one piece of previously prepared encapsulated cortical tissue and place it in the pocket. Then use six O braided absorbable suture to suture the fascia before repeating the co-transplant on the contra-lateral side.Close the dorsal wall with four O monofilament absorbable suture in simple interrupted stitches. Inject lidocaine subcutaneous at the incision site. Then use a sterile alcohol prep to clean the skin and carry out post-operative care according to the text protocol.As seen here, two weeks after bilateral engraftment of E4-ORF1 treated human umbilical vein endothelial cells into NSG female mice, functionally perfused vessels formed from GFP labeled exogenous ECs or ExECs at the interface of host tissue and the graft. ExECs formed functional vessels when co-transplanted with ovarian tissue in cortical pieces co-transplanted with the mouse or human ExECs, increase the number of surviving follicles relative to control grafts. This increase in follicle development was associated with a decreased fibrotic area in ExEC assisted grafts.Final fibrotic area values were calculated for each graft as the average of three sections analyzed. Importantly, grafts that were co-transplanted with human foreskin fibroblasts showed poor tissue viability and relatively few follicles. 14 weeks after engraftment, mice were stimulated with menotropins.And the progression of follicle growth was monitored via MRI. Developing follicles were noted in both control and ExEC assisted grafts. However, more and larger sized follicles were noticed in the ExEC assisted grafts.Compared to antral follicles derived from the same patient's tissue, the granulosa cell layer in the more advanced follicle displayed increased expression of the ovulatory markers CD44 and HABP1, as well as increased cell death and reduced proliferation. After watching this video, you will understand how to perform co-transplantation of cryopreserved ovarian tissue with endothelial cells in a fibrin clot. And once mastered, you should be able to complete this protocol in less than one hour.Building on this approach, endothelial cells can be engineered to express any combination of secreted factors, thereby enabling interrogation of signaling pathways that may be relevant to fertility preservation, folliculogenesis, and/or tissue transplantation.

Research

JoVE Journal

Bioengineering

Sensing of Barrier Tissue Disruption with an Organic Electrochemical Transistor

Organik Elektrokimyasal Transistör Bariyer Doku bozulması ve Algılama

The gastrointestinal tract is an example of barrier tissue that provides a physical barrier against entry of pathogens and toxins, while allowing the ...

Biyomühendislik

Tissue Engineering of Tumor Stromal Microenvironment with Application to Cancer Cell Invasion

Kanser Hücre Invasion Uygulama ile Tümör Stromal mikroçevresinin Doku Mühendisliği

3D organotypic cultures of epithelial cells on a matrix embedded with mesenchymal cells are widely used to study epithelial cell differentiation and ...

Generation and Grafting of Tissue-engineered Vessels in a Mouse Model

Bir Fare Modeli Doku mühendisliği Kapların Üretimi ve Tırnak

The construction of vascular conduits is a fundamental strategy for surgical repair of damaged and injured vessels resulting from cardiovascular diseases. ...

Production, Characterization and Potential Uses of a 3D Tissue-engineered Human Esophageal Mucosal Model

3D Doku mühendisliği İnsan Özofagus Mukozal Modeli Üretimi, Karakterizasyonu ve Potansiyel Kullanımları

The incidence of both esophageal adenocarcinoma and its precursor, Barrett&#8217;s Metaplasia, are rising rapidly in the western world. Furthermore ...

Delivery of Proteins, Peptides or Cell-impermeable Small Molecules into Live Cells by Incubation with the Endosomolytic Reagent dfTAT

Endosomolitik Reaktif dfTAT ile kuluçkaya yatırılması ile Canlı hücreler içine proteinler, peptitler ya da hücre-geçirgen olmayan küçük moleküller teslimi

Macromolecular delivery strategies typically utilize the endocytic pathway as a route of cellular entry. However, endosomal entrapment severely limits the ...

Automated Contraction Analysis of Human Engineered Heart Tissue for Cardiac Drug Safety Screening

Kardiyak İlaç güvenlik Taraması için insan mühendisliği kalp dokusunun Otomatik Büzülme Analizi

Cardiac tissue engineering describes techniques to constitute three dimensional force-generating engineered tissues. For the implementation of these ...

Microdissection of Primary Renal Tissue Segments and Incorporation with Novel Scaffold-free Construct Technology

Mikrodiseksiyon birincil böbrek doku parçaları ve roman iskele-Alerjik yapı teknolojisi ile birleşme

Kidney transplantation is now a mainstream therapy for end-stage renal disease. However, with approximately 96,000 people on the waiting list and only ...

A Novel Tenorrhaphy Suture Technique with Tissue Engineered Collagen Graft to Repair Large Tendon Defects

Büyük Tendon Kusurlarını Onarmak İçin Doku Mühendisliği Kollajen Greftli Yeni Bir Tenorhaphy Dikiş Tekniği

Surgical management of large tendon defects with tendon grafts is challenging, as there are a finite number of sites where donors can be readily ...

Three-dimensional Patterning of Engineered Biofilms with a Do-it-yourself Bioprinter

Bir do-it-yourself Bioprinter ile tasarlanan Biyosilmların üç boyutlu deseni

Biofilms are aggregates of bacteria embedded in a self-produced spatially-patterned extracellular matrix. Bacteria within a biofilm develop enhanced ...

Establishing Single-Cell Based Co-Cultures in a Deterministic Manner with a Microfluidic Chip

Mikroakışkan Çiple Deterministik Bir Şekilde Tek Hücreli Ortak Kültürlerin Oluşturulması

Cell co-culture assays have been widely used for studying cell-cell interactions between different cell types to better understand the biology of diseases ...