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DOI :
10.3791/57475-v
•
6:26 min
March 21st, 2018
Chapters
0:04
Title
0:40
Dissection and Isolation of Jejunum
2:40
Immunofluorescence of Tuft Cells Using Free-floating Cryosections
4:42
Results: Representative Fluorescence Images of Mouse Intestinal Tuft Cells
5:55
Conclusion
库加et . 发现在酪氨酸 1798 (pY1798) 中, 抗肌动蛋白结合蛋白 girdin 磷酸化的特定抗体可用于标记一簇细胞 (TCs)。该协议允许使用 pY1798 抗体的自由漂浮空肠 cryosections 免疫荧光染色对 TCs 进行鲁棒可视化。
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