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DOI :
10.3791/57479-v
•
9:02 min
April 20th, 2018
Chapters
0:04
Title
0:46
Split-BioID for Proteomics Studies
2:54
Streptavidin Pulldown
5:03
SDS-PAGE and Western Blotting
6:21
Results: Protein Expression and Biotinylation Through Split-BioID
8:04
Conclusion
我们提供了一步一步的协议为分裂-BioID, 一种蛋白质片段互补试验的基础上的接近标记技术 BioID。激活在两个给定的蛋白质的相互作用, 它允许蛋白质组学分析的上下文相关的蛋白质复合物在他们的本机细胞环境。该方法简单, 成本效益高, 只需要标准的实验室设备。
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