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DOI :
10.3791/57486-v
March 16th, 2018
Chapters
0:04
Title
1:08
Bone Marrow-derived Macrophage (BMM) Isolation and Differentiation
4:05
BMM Coverslip Plating
5:22
L. amazonensis Infection and Parasite Quantification
7:21
Results: Representative BMM Infection Analyses
9:06
Conclusion
所有致病性的利什曼原虫物种在其脊椎动物宿主的巨噬细胞内栖息和复制。在这里, 我们提出了一个协议, 以感染小鼠骨髓源性巨噬细胞的文化与利什曼原虫, 然后精确定量的细胞内生长动力学。该方法对于研究影响寄主病原体相互作用和利什曼原虫毒力的个体因素非常有用。
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