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DOI :
10.3791/57486-v
March 16th, 2018
Chapters
0:04
Title
1:08
Bone Marrow-derived Macrophage (BMM) Isolation and Differentiation
4:05
BMM Coverslip Plating
5:22
L. amazonensis Infection and Parasite Quantification
7:21
Results: Representative BMM Infection Analyses
9:06
Conclusion
すべての病原性リーシュ マニア原虫種に存在し、脊椎動物のホストのマクロファージ内で複製。ここでは、リーシュ マニア、続いて細胞増殖動態の正確な定量培養マウス骨髄由来マクロファージに感染するプロトコルを提案する.このメソッドは、リーシュ マニアの病原性と宿主-病原体相互作用に影響を与える個々 の要因を調べる場合に役立ちます。
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