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DOI :
10.3791/57492-v
April 11th, 2018
Chapters
0:04
Title
0:41
Passage Human Nasal Epithelial Cells
2:45
Seeding Cells for HNE Spheroid Cultures
4:37
Culture Differentiated and Mature HNE Spheroids
5:27
Results: Individualized CFTR Study Through the Generation of HNE Spheroids
7:01
Conclusion
这里我们描述了一种生成三维的人鼻上皮细胞的球形培养方法。球体被刺激以驱动囊性纤维化跨膜电导调节剂 (CFTR) 依赖的离子和液体分泌物, 将球体腔内大小的变化量化为 CFTR 功能的代表。
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