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DOI :
10.3791/57506-v
•
11:22 min
June 27th, 2018
Chapters
0:04
Title
0:53
Cell Lysis and Purification of His6-SUMO Httex1 Fusion Protein by Immobilized Metal Affinity Chromatography (IMAC)
2:56
Cleavage of the His6-SUMO-tag and HPLC Purification
6:06
Disaggregation and Resolubilization of Httex1 Proteins
7:53
Results: Characterization of Native, Untagged Huntingtin Exon1 Monomer and Fibrils Prepared via SUMO Fusion
9:52
Conclusion
在这里, 我们提出了一个健壮和优化的协议, 以生产毫克数量的本地, 无标签单体和纤维的 exon1 的杭丁顿蛋白蛋白 (Httex1) 的基础上的瞬态融合的小泛素相关修饰剂 (相扑)。
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