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DOI :
10.3791/57506-v
•
11:22 min
June 27th, 2018
Chapters
0:04
Title
0:53
Cell Lysis and Purification of His6-SUMO Httex1 Fusion Protein by Immobilized Metal Affinity Chromatography (IMAC)
2:56
Cleavage of the His6-SUMO-tag and HPLC Purification
6:06
Disaggregation and Resolubilization of Httex1 Proteins
7:53
Results: Characterization of Native, Untagged Huntingtin Exon1 Monomer and Fibrils Prepared via SUMO Fusion
9:52
Conclusion
ここでは、ネイティブなタグ無料モノマーのミリグラム量と小さなユビキチン関連修飾子 (相撲) の一時的な融合に基づくハンチンチン蛋白質 (Httex1) の exon1 の線維の生産のための堅牢な最適化されたプロトコルを提案する.
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